pU6-IGSF8-sgRNA2-Cas9-EGFP-Pur

pU6-IGSF8-sgRNA2-Cas9-EGFP-Puro plasmid

PVT109844

pU6-IGSF8-sgRNA2-Cas9-EGFP-Puro plasmid Information

Plasmid name: pU6-IGSF8(human)-sgRNA2-Cas9-EGFP-Puro
Alias: GGTACCGCATGGTAGTAGG
Gene lenght:
Host: Mammalian cells
Use(s): Gene knockout
Fragment type: Cas9
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pU6-IGSF8-sgRNA2-Cas9-EGFP-Puro plasmid Description

pU6-IGSF8-sgRNA2-Cas9-EGFP-Puro This plasmid is suitable for gene knockout in mammalian cells.

pU6-IGSF8-sgRNA2-Cas9-EGFP-Puro plasmid Sequence

1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga 241 cgaaacaccg gGGTACCGCA TGGTAGTAGG gttttagagc tagaaatagc aagttaaaat 301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga 361 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca 421 gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac 481 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac 541 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 601 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc 661 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 721 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc 781 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg 841 gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc 901 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga 961 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga 1021 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga 1081 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat 1141 tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat 1201 tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc accatggact 1261 ataaggacca cgacggagac tacaaggatc atgatattga ttacaaagac gatgacgata 1321 agatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga 1381 agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg 1441 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca 1501 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc 1561 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag 1621 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt 1681 ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg 1741 acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg 1801 acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt 1861 tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc 1921 tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca 1981 gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa 2041 atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc 2101 tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac 2161 tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg 2221 accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg 2281 acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga 2341 gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc 2401 ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg 2461 acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg 2521 acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga 2581 ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc 2641 ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc 2701 tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct 2761 ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca 2821 agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca 2881 acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc 2941 tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc 3001 agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc 3061 tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg 3121 aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca 3181 aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga 3241 cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg 3301 acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc 3361 ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga 3421 agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct 3481 ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca 3541 ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg 3601 tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg 3661 ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga 3721 tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca 3781 cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg 3841 tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc 3901 agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc 3961 ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc 4021 ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg 4081 agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa 4141 cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg 4201 acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt 4261 ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg 4321 cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc 4381 tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca 4441 agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga 4501 actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg 4561 agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc 4621 ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg 4681 gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga 4741 aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc 4801 tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc 4861 tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag 4921 ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt 4981 tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa 5041 acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga 5101 agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc 5161 actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg 5221 acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag 5281 agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct 5341 tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg 5401 acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc 5461 agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa 5521 aggaattcgg cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga 5581 atcctggccc agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 5641 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 5701 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 5761 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 5821 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 5881 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 5941 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 6001 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 6061 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 6121 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 6181 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 6241 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 6301 acaaggaatt ctaactagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc 6361 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 6421 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 6481 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc 6541 tggggagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg 6601 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc 6661 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct 6721 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt 6781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 6841 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 6901 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 6961 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga 7021 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 7081 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg 7141 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 7201 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc 7261 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 7321 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagGTG TGTCAGTTAG 7381 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 7441 AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 7501 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA 7561 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG 7621 AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG 7681 GCCTAGGCTT TTGCAAAAAG CTCCCgggat gACCGAGTAC AAGCCCACGG TGCGCCTCGC 7741 CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC 7801 CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA 7861 ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC 7921 CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT 7981 CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG 8041 CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGAGTCTC 8101 GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC 8161 CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA 8221 CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG 8281 GTGCATGACC CGCAAGCCCG GTGCCTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC 8341 CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA 8401 GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA 8461 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA 8521 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAa cgcgcgagac gaaagggcct 8581 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg 8641 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 8701 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 8761 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 8821 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 8881 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 8941 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 9001 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 9061 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 9121 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 9181 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 9241 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 9301 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 9361 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 9421 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 9481 tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 9541 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 9601 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 9661 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 9721 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 9781 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 9841 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 9901 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 9961 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 10021 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 10081 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 10141 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 10201 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 10261 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 10321 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 10381 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 10441 tcacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.