pLV3-U6-PELI3-shRNA1-CopGFP-Ze

pLV3-U6-PELI3-shRNA1-CopGFP-Zeo plasmid

PVT109842

pLV3-U6-PELI3-shRNA1-CopGFP-Zeo plasmid Information

Plasmid name: pLV3-U6-PELI3(human)-shRNA1-CopGFP-Zeo
Alias: GCTTCGATGCCTCTAGCAA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene interference
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Zeo
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLV3-U6-PELI3-shRNA1-CopGFP-Zeo plasmid Description

pLV3-U6-PELI3-shRNA1-CopGFP-Zeo It is a plasmid suitable for gene interference in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-U6-PELI3-shRNA1-CopGFP-Zeo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgaattc gGCTTCGATG CCTCTAGCAA ttcaagagaT 2221 TGCTAGAGGC ATCGAAGCtt ttttggatcc gcggccgcGa aggatctgcg atcgctccgg 2281 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 2341 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 2401 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 2461 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc 2521 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag 2581 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag 2641 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact 2701 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt 2761 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat 2821 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct 2881 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat 2941 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca 3001 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt 3061 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg 3121 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt 3181 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg 3241 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt 3301 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat 3361 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt 3421 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc 3481 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga 3541 cggcaccgcc ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg 3601 cggtgacgtg gaggagaatc ccggccctAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT 3661 CACCGCGCGC GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG 3721 GGACTTCGTG GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG 3781 CGCGGTCCAG GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT 3841 GGACGAGCTG TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG 3901 GCCGGCCATG ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC 3961 GGCCGGCAAC TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGAgtcgaca atcaacctct 4021 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 4081 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 4141 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 4201 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 4261 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 4321 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 4381 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 4441 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 4501 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 4561 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga 4621 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 4681 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag 4741 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 4801 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 4861 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 4921 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 4981 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 5041 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 5101 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 5161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 5221 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc 5281 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 5341 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 5401 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 5461 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 5521 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 5581 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 5641 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 5701 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5761 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5821 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5881 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 5941 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 6001 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 6061 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 6121 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 6241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 6301 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 6361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 6421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 6481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 6541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 6601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 6661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 6721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 6781 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 6841 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 6901 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 6961 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 7021 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 7081 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7141 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7201 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 7261 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 7321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 7381 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 7441 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 7501 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 7561 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 7621 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 7681 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 7741 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 7801 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 7861 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.