pCMV-WRAP53-EYFP-Neo plasmid

pCMV-WRAP53-EYFP-Neo plasmid

PVT109761

pCMV-WRAP53-EYFP-Neo plasmid Information

Plasmid name: pCMV-WRAP53(human)-EYFP-Neo
Alias: NM_018081.2;DKCB3; TCAB1; WDR79
Gene lenght: 1644
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Yellow
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-WRAP53-EYFP-Neo plasmid Description

pCMV-WRAP53-EYFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-WRAP53-EYFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcGCCACC atgaagactt tggagactca 661 accgttagct ccggactgct gtccttcaga ccaggaccca gctccagccc atccttctcc 721 ccacgcttcc ccgatgaata aaaatgcgga ctctgaactg atgccaccgc ctcccgaaag 781 gggggatccg ccccggttgt ccccagatcc tgtggctggc tcagctgtgt cccaggagct 841 acgggagggg gacccagttt ctctctccac tcccctggaa acagagtttg gttcccctag 901 tgagttgagt cctcgaatcg aggagcaaga actttctgaa aatacaagcc ttcctgcaga 961 agaagcaaac gggagccttt ctgaagaaga agcgaacggg ccagagttgg ggtctggaaa 1021 agccatggaa gatacctctg gggaacccgc tgcagaggac gagggagaca ccgcttggaa 1081 ctacagcttc tcccagctgc ctcgatttct cagtggttcc tggtcagagt tcagcaccca 1141 acctgagaac ttcttgaaag gctgtaagtg ggctcctgac ggttcctgca tcttgaccaa 1201 tagtgctgat aacatcttgc gaatttataa cctgccccca gagctgtacc atgaggggga 1261 gcaggtggaa tatgcagaaa tggtccctgt ccttcgaatg gtggaaggtg ataccatcta 1321 tgattactgc tggtattctc tgatgtcctc agcccagcca gacacctcct acgtggccag 1381 cagcagccgg gagaacccga ttcatatctg ggacgcattc actggagagc tccgggcttc 1441 ctttcgcgcc tacaaccacc tggatgagct gacggcagcc cattcgctct gcttctcccc 1501 ggatggctcc cagctcttct gtggcttcaa ccggactgtg cgtgtttttt ccacggcccg 1561 gcctggccga gactgcgagg tccgagccac atttgcaaaa aagcagggcc agagcggcat 1621 catctcctgc atagccttca gcccagccca gcccctctat gcctgtggct cctacggccg 1681 ctccctgggt ctgtatgcct gggatgatgg ctcccctctc gccttgctgg gagggcacca 1741 agggggcatc acccacctct gctttcatcc cgatggcaac cgcttcttct caggagcccg 1801 caaggatgct gagctcctgt gctgggatct ccggcagtct ggttacccac tgtggtccct 1861 gggtcgagag gtgaccacca atcagcgcat ctacttcgat ctggacccga ccgggcagtt 1921 cctagtgagt ggcagcacga gcggggctgt ctctgtgtgg gacacggacg ggcctggcaa 1981 tgatgggaag ccggagcccg tgttgagttt tctgccccag aaggactgca ccaatggcgt 2041 gagcctgcac cctagcctgc ctctcctggc cactgcctcc ggtcagcgtg tgtttcctga 2101 gcccacagag agtggggacg aaggagagga gctgggcctt cccttgctct ccacgcgcca 2161 cgtccacctt gaatgtcggc ttcagctctg gtggtgtggg ggggcgccag actccagcat 2221 ccctgatgat caccagggcg agaaagggca gggaggaacg gagggaggtg tgggtgagct 2281 gataGAATTC ggtggaggcg gatcaggagg cggtggatct CCggatccac cggtcgccac 2341 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 2401 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 2461 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 2521 cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa 2581 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 2641 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 2701 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 2761 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 2821 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 2881 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 2941 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 3001 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 3061 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 3121 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 3181 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3301 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 3361 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 3421 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 3481 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 3541 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 3601 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 3661 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 3721 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 3781 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 3841 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 3901 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 3961 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 4021 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 4081 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 4141 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 4201 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 4261 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 4321 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 4381 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 4441 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 4501 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 4561 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 4621 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 4681 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 4741 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 4801 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 4861 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 4921 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 4981 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 5041 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 5101 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 5161 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 5221 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 5281 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 5341 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 5401 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 5461 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 5521 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 5581 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 5641 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 5701 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5761 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5821 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 5881 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5941 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 6001 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 6061 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 6121 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 6181 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 6241 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 6301 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 6361 cctgattctg tggataaccg tattaccgcc atgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.