pLV3-CMV-Mia-3×FLAG-CopGFP-Pur

pLV3-CMV-Mia-3×FLAG-CopGFP-Puro plasmid

PVT109624

pLV3-CMV-Mia-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Mia(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_019394.3;CD-RAP; Cdrap; Mia1
Gene lenght: 390
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Mia-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Mia-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Mia-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg tgtggtcccc agtgctcctt 2341 ggcatcgtcg tcttgtctgt tttttcaggg cctagcaggg ctgatcgagc tatgcccaag 2401 ctggctgact ggaagctgtg tgcggacgag gaatgcagcc atcctatctc catggctgtg 2461 gccctccagg actacgtggc ccctgattgc cgcttcttga ctatatatag gggccaagtg 2521 gtgtatgtct tctccaagtt gaagggccgt gggcgccttt tctggggagg cagtgttcag 2581 ggaggttact atggagacct ggcagcccgc ctgggctatt tccccagtag cattgtccgg 2641 gaggacctga ctctgaaacc tggcaaaatt gatatgaaga ccgatcaatg ggatttctac 2701 tgccagggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 2761 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 2821 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 2881 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 2941 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 3001 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 3061 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 3121 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 3181 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 3241 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 3301 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 3361 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 3421 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 3481 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 3541 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 3601 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 3661 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 3721 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 3781 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 3841 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 3901 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 3961 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 4021 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 4081 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 4141 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4201 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4261 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 4321 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 4381 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 4441 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 4501 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 4561 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 4621 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 4681 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 4741 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 4801 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 4861 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 4921 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 4981 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5041 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5101 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 5161 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 5221 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 5281 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 5341 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 5401 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 5461 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 5521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 5581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 5641 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 5701 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 5761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 5821 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 5881 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 5941 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 6001 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 6061 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6121 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6181 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6241 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6301 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6361 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 6421 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 6481 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 6541 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 6601 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 6661 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 6721 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 6781 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 6841 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 6901 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 6961 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7021 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7081 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7141 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7201 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7261 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7321 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 7381 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 7441 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 7501 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 7561 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 7621 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 7681 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 7741 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 7801 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 7861 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 7921 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 7981 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8041 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8101 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8161 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 8221 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 8281 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 8341 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 8401 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 8461 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 8521 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 8581 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 8641 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.