pLV3-CMV-DDR1-Myc-EF1a-CopGFP-

pLV3-CMV-DDR1-Myc-EF1a-CopGFP-T2A-Puro plasmid

PVT109242

pLV3-CMV-DDR1-Myc-EF1a-CopGFP-T2A-Puro plasmid Information

Plasmid name: pLV3-CMV-DDR1(human)-Myc-EF1a-CopGFP-T2A-Puro
Alias: NM_001297654.2;CAK; CD167; DDR; EDDR1; HGK2; MCK10; NEP;NTRK4; PTK3; PTK3A; RTK6; TRKE
Gene lenght: 2739
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-DDR1-Myc-EF1a-CopGFP-T2A-Puro plasmid Description

pLV3-CMV-DDR1-Myc-EF1a-CopGFP-T2A-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-DDR1-Myc-EF1a-CopGFP-T2A-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg gaccagaggc cctgtcatct ttactgctgc tgctcttggt 2341 ggcaagtgga gatgctgaca tgaagggaca ttttgatcct gccaagtgcc gctatgccct 2401 gggcatgcag gaccggacca tcccagacag tgacatctct gcttccagct cctggtcaga 2461 ttccactgcc gcccgccaca gcaggttgga gagcagtgac ggggatgggg cctggtgccc 2521 cgcagggtcg gtgtttccca aggaggagga gtacttgcag gtggatctac aacgactgca 2581 cctggtggct ctggtgggca cccagggacg gcatgccggg ggcctgggca aggagttctc 2641 ccggagctac cggctgcgtt actcccggga tggtcgccgc tggatgggct ggaaggaccg 2701 ctggggtcag gaggtgatct caggcaatga ggaccctgag ggagtggtgc tgaaggacct 2761 tgggcccccc atggttgccc gactggttcg cttctacccc cgggctgacc gggtcatgag 2821 cgtctgtctg cgggtagagc tctatggctg cctctggagg gatggactcc tgtcttacac 2881 cgcccctgtg gggcagacaa tgtatttatc tgaggccgtg tacctcaacg actccaccta 2941 tgacggacat accgtgggcg gactgcagta tgggggtctg ggccagctgg cagatggtgt 3001 ggtggggctg gatgacttta ggaagagtca ggagctgcgg gtctggccag gctatgacta 3061 tgtgggatgg agcaaccaca gcttctccag tggctatgtg gagatggagt ttgagtttga 3121 ccggctgagg gccttccagg ctatgcaggt ccactgtaac aacatgcaca cgctgggagc 3181 ccgtctgcct ggcggggtgg aatgtcgctt ccggcgtggc cctgccatgg cctgggaggg 3241 ggagcccatg cgccacaacc tagggggcaa cctgggggac cccagagccc gggctgtctc 3301 agtgcccctt ggcggccgtg tggctcgctt tctgcagtgc cgcttcctct ttgcggggcc 3361 ctggttactc ttcagcgaaa tctccttcat ctctgatgtg gtgaacaatt cctctccggc 3421 actgggaggc accttcccgc cagccccctg gtggccgcct ggcccacctc ccaccaactt 3481 cagcagcttg gagctggagc ccagaggcca gcagcccgtg gccaaggccg aggggagccc 3541 gaccgccatc ctcatcggct gcctggtggc catcatcctg ctcctgctgc tcatcattgc 3601 cctcatgctc tggcggctgc actggcgcag gctcctcagc aaggctgaac ggagggtgtt 3661 ggaagaggag ctgacggttc acctctctgt ccctggggac actatcctca tcaacaaccg 3721 cccaggtcct agagagccac ccccgtacca ggagccccgg cctcgtggga atccgcccca 3781 ctccgctccc tgtgtcccca atggctctgc gttgctgctc tccaatccag cctaccgcct 3841 ccttctggcc acttacgccc gtccccctcg aggcccgggc ccccccacac ccgcctgggc 3901 caaacccacc aacacccagg cctacagtgg ggactatatg gagcctgaga agccaggcgc 3961 cccgcttctg cccccacctc cccagaacag cgtcccccat tatgccgagg ctgacattgt 4021 taccctgcag ggcgtcaccg ggggcaacac ctatgctgtg cctgcactgc ccccaggggc 4081 agtcggggat gggcccccca gagtggattt ccctcgatct cgactccgct tcaaggagaa 4141 gcttggcgag ggccagtttg gggaggtgca cctgtgtgag gtcgacagcc ctcaagatct 4201 ggttagtctt gatttccccc ttaatgtgcg taagggacac cctttgctgg tagctgtcaa 4261 gatcttacgg ccagatgcca ccaagaatgc caggaatgat ttcctgaaag aggtgaagat 4321 catgtcgagg ctcaaggacc caaacatcat tcggctgctg ggcgtgtgtg tgcaggacga 4381 ccccctctgc atgattactg actacatgga gaacggcgac ctcaaccagt tcctcagtgc 4441 ccaccagctg gaggacaagg cagccgaggg ggcccctggg gacgggcagg ctgcgcaggg 4501 gcccaccatc agctacccaa tgctgctgca tgtggcagcc cagatcgcct ccggcatgcg 4561 ctatctggcc acactcaact ttgtacatcg ggacctggcc acgcggaact gcctagttgg 4621 ggaaaatttc accatcaaaa tcgcagactt tggcatgagc cggaacctct atgctgggga 4681 ctattaccgt gtgcagggcc gggcagtgct gcccatccgc tggatggcct gggagtgcat 4741 cctcatgggg aagttcacga ctgcgagtga cgtgtgggcc tttggtgtga ccctgtggga 4801 ggtgctgatg ctctgtaggg cccagccctt tgggcagctc accgacgagc aggtcatcga 4861 gaacgcgggg gagttcttcc gggaccaggg ccggcaggtg tacctgtccc ggccgcctgc 4921 ctgcccgcag ggcctatatg agctgatgct tcggtgctgg agccgggagt ctgagcagcg 4981 accacccttt tcccagctgc atcggttcct ggcagaggat gcactcaaca cggtgggatc 5041 cGAACAAAAA CTCATCTCAG AAGAGGATCT Gtgagcggcc gcgaaggatc tgcgatcgct 5101 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 5161 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 5221 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 5281 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag 5341 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt 5401 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg 5461 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta 5521 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt 5581 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca 5641 ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca 5701 ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc 5761 gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga 5821 gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc 5881 ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg 5941 acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg 6001 acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca 6061 tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca 6121 gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc 6181 acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg 6241 ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc 6301 acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg 6361 tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa 6421 catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc 6481 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 6541 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 6601 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 6661 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 6721 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 6781 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 6841 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 6901 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 6961 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 7021 cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg 7081 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 7141 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 7201 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 7261 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 7321 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 7381 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 7441 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 7501 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 7561 tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct 7621 ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag 7681 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 7741 aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 7801 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 7861 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 7921 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 7981 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 8041 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 8101 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 8161 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 8221 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 8281 cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct 8341 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 8401 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 8461 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 8521 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 8581 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 8641 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 8701 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 8761 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 8821 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 8881 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 8941 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 9001 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 9061 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 9121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 9181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 9241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 9301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 9361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 9421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 9481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 9541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 9601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 9661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 9721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 9781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 9841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 9901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 9961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 10021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 10081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 10141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 10201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 10261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 10321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 10381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 10441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 10501 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 10561 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 10621 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 10681 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 10741 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 10801 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 10861 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 10921 gtcacgacgt tgtaaaacga cggccagtgc caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.