pLV2-CMV-RPN2-EGFP-mCherry pla

pLV2-CMV-RPN2-EGFP-mCherry plasmid

PVT108777

pLV2-CMV-RPN2-EGFP-mCherry plasmid Information

Plasmid name: pLV2-CMV-RPN2(human)-EGFP-mCherry
Alias: NM_001324299.2;RIBIIR; RPN-II; RPNII; SWP1
Gene lenght: 1845
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red,Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-RPN2-EGFP-mCherry plasmid Description

pLV2-CMV-RPN2-EGFP-mCherry It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-RPN2-EGFP-mCherry plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggcg 2821 ccgccgggtt caagcactgt cttcctgttg gccctgacaa tcatagccag cacctgggct 2881 ctgacgccca ctcactacct caccaagcat gacgtggaga gactaaaagc ctcgctggat 2941 cgccctttca caaatttgga atctgccttc tactccatcg tgggactcag cagccttggt 3001 gctcaggtgc cagatgcaaa gaaagcatgt acctacatca gatctaacct tgatcccagc 3061 aatgtggatt ccctcttcta cgctgcccag gccagccagg ccctctcagg atgtgagatc 3121 tctatttcaa atgagaccaa agatctgctt ctggcagctg tcagtgagga ctcatctgtt 3181 acccagatct accatgcagt tgcagctcta agtggctttg gccttccctt ggcatcccaa 3241 gaagcactca gtgcccttac tgctcgtctc agcaaggagg agactgtgct ggcaacagtc 3301 caggctctgc agacagcatc ccacctgtcc cagcaggctg acctgaggag catcgtggag 3361 gagattgagg accttgttgc tcgcctggat gaactcgggg gcgtgtatct ccagtttgaa 3421 gaaggactgg aaacaacagc gttatttgtg gctgccacct acaagctcat ggatcatgtg 3481 gggactgagc catccattaa ggaggatcag gtcatccagc tgatgaacgc gatcttcagc 3541 aagaagaact ttgagtccct ctccgaagcc ttcagcgtgg cctctgcagc tgctgtgctc 3601 tcgcataatc gctaccacgt gccagttgtg gttgtgcctg agggctctgc ttccgacact 3661 catgaacagg ctatcttgcg gttgcaagtc accaatgttc tgtctcagcc tctgactcag 3721 gccactgtta aactagaaca tgctaaatct gttgcttcca gagccactgt cctccagaag 3781 acatccttca cccctgtagg ggatgttttt gaactaaatt tcatgaacgt caaattttcc 3841 agtggttatt atgacttcct tgtcgaagtt gaaggtgaca accggtatat tgcaaatacc 3901 gtagagctca gagtcaagat ctccactgaa gttggcatca caaatgttga tctttccacc 3961 gtggataagg atcagagcat tgcacccaaa actacccggg tgacataccc agccaaagcc 4021 aagggcacat tcatcgcaga cagccaccag aacttcgcct tgttcttcca gctggtagat 4081 gtgaacactg gtgctgaact cactcctcac cagacatttg tccgactcca taaccagaag 4141 actggccagg aagtggtgtt tgttgccgag ccagacaaca agaacgtgta caagtttgaa 4201 ctggatacct ctgaaagaaa gattgaattt gactctgcct ctggcaccta cactctctac 4261 ttaatcattg gagatgccac tttgaagaac ccaatcctct ggaatgtggc tgatgtggtc 4321 atcaagttcc ctgaggaaga agctccctcg actgtcttgt cccagaacct tttcactcca 4381 aaacaggaaa ttcagtggat ccggattggt gccaatgtct ccaacttcac ttttgctcct 4441 agcacgatta tatttcacct gggacatgct gctatgctgg gactcatgta tgtctactgg 4501 actcagctca acatgttcca gaccttgaag tacctggcca tcctgggcag tgtgacgttt 4561 ctggctggca atcggatgct ggcccagcag gcagtcaaga ggatcgctgc agagcagagc 4621 agtagattgg caaaatatag gacactacga acagcacatg gcggtggagg cagtggtgga 4681 ggcggtagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 4741 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 4801 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 4861 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 4921 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 4981 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 5041 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 5101 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 5161 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 5221 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 5281 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 5341 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 5401 tacaagtaag gatcccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc 5461 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt 5521 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt 5581 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg 5641 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca 5701 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg 5761 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctcacc 5821 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct 5881 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag 5941 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggtgagc 6001 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg 6061 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 6121 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg 6181 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac 6241 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 6301 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc 6361 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag 6421 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag 6481 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 6541 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 6601 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 6661 ggccgccact ccaccggcgg catggacgag ctgtacaagt gaacgcgtct ggaacaatca 6721 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 6781 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 6841 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 6901 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 6961 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 7021 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 7081 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 7141 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 7201 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 7261 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc 7321 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 7381 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 7441 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 7501 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 7561 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 7621 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 7681 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 7741 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 7801 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 7861 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 7921 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 7981 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 8041 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 8101 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 8161 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg 8221 acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 8281 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 8341 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 8401 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 8461 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 8521 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 8581 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 8641 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 8701 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 8761 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 8821 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 8881 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 8941 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 9001 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 9061 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 9121 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 9181 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 9241 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 9301 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 9361 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 9421 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 9481 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 9541 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 9601 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 9661 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 9721 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 9781 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 9841 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 9901 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 9961 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 10021 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 10081 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 10141 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 10201 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 10261 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 10321 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 10381 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg 10441 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 10501 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 10561 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 10621 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg 10681 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact 10741 tagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.