pCMV-Mtr-3×FLAG-Neo plasmid

pCMV-Mtr-3×FLAG-Neo plasmid

PVT111445

pCMV-Mtr-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Mtr(mouse)-3×FLAG-Neo
Alias: NM_001081128.3;D830038K18Rik; MS
Gene lenght: 3759
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Mtr-3×FLAG-Neo plasmid Description

pCMV-Mtr-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Mtr-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCgcc 961 accatgaaga aaaccctgca ggatgagatt gaagccattc ttcggaagag gatcatggtg 1021 ctagacggtg ggatggggac catgatccag cggtacaaac taagtgaaga acatttccaa 1081 ggtcaagagt ttaaagatca cagcaggcca ctgaaaggca acaatgacat cttaagtata 1141 actcagcctg atattattta ccagattcat aaggaatact tgctggctgg agcagatatc 1201 atcgaaacaa acactttcag cagtactagt atcgcccagg ctgactatgg ccttgaacac 1261 ttggcctacc ggatgaacaa gtgctctgca gatgtggcca gaaaagccgc tgaggagata 1321 actctgcaga caggagtcaa gaggtttgtg gctggagctc tgggtccgac taataagaca 1381 ctttctgtct ccccatctgt ggaaaggcca gattatagga acatcacatt tgatgagctt 1441 gttgacgcat accaggagca ggccaaggga ctgctggatg gcagggtcga catcttactc 1501 attgaaacaa tttttgatac agctaatgcc aaagcagcct tgtttgcgat ccagaacctc 1561 tttgaagaga attatgctcc tcctcggcct atctttattt ctgggaccat tgttgataaa 1621 agtggaagga ctctttctgg gcagacggga gaggcttttg tcaccagcgt gtctcattcg 1681 gaccccctgt gcattggatt aaattgttct ctgggtgcag ctgaaatgag gcctttcatt 1741 gaaacaattg gaaaatgtac aacagcctat gtcctctgtt acccaaatgc aggtcttccc 1801 aacacttttg gcgactatga tgaaacacca tccacgatgg ccacgcacct gaaggacttt 1861 gctgtggatg gcttggtgaa tatagttggt ggatgctgtg gttcaacacc tgatcacatc 1921 agggaaattg cggaagctgt gaaaaagtgt aagccaagag tcccccctgc tagtgttttt 1981 gaaggacata tgttactgtc tggtctagag cccttcagga ttggaccata caccaacttt 2041 gttaacattg gagagcgctg taatgtggca ggatccagga aatttgctaa actcatcatg 2101 gcaggaaact atgaagaagc cctgagcatt gccaaagcac aggtggaaat gggagctcag 2161 gtgctcgata tcaacatgga cgacggcatg ctggatggtc ccagtgcaat gaccagattt 2221 tgcaactcca ttgcttctga gcctgacatt gccaaggtgc ccctgtgcat tgactcttct 2281 aactttgccg tgattgaagc tgggttgaag tgctgccaag ggaagtgcat agtcaacagc 2341 atcagcctca aggaggggga gggggacttc ctggagaagg cccggaagat taagaagttt 2401 ggagctgctg tggtggttat ggcttttgat gaggaaggac aagcaacaga aacagatgtt 2461 aaagtcaatg tgtgcaccag agcttaccat ctacttgtgg acaaagtggg cttcaatcca 2521 aatgacatca tctttgaccc caacatcctt accattggta ctggaatgga ggaacataac 2581 ttgtatgcta tcaattttat ccatgcaaca agagtcatta aggaaacctt accaggagtc 2641 agaataagtg gaggtctttc caacttatcc ttctccttcc gagggatgga agccattcga 2701 gaagcaatgc atggtgtgtt cctttatcac gcgatcaagt ttggtatgga catggggata 2761 gtcaatgccg gcaacctccc tgtgtatgat gctatccaca aggaccttct tcagctctgt 2821 gaagacctca tctggaacaa agactctgag gctactgaga aactcttgcg atatgcccag 2881 actcatggca caggagggaa gaaagtcatc cagacagatg agtggaggaa tggctccatt 2941 gaagagcgcc tggagtatgc tcttgtgaag ggcattgaaa agcacattgt tgaagatact 3001 gaagaagcca ggttaaacgg ggaaaaatac cctcggcctc tgaatataat tgaagggccc 3061 ctaatgaatg gcatgaaagt ggttggtgat ctttttggag ctggaaagat gtttctacct 3121 caggttataa agtcagcccg tgtcatgaag aaggctgttg gccaccttat ccctttcatg 3181 gaaaaagaaa gagaagaagc cagattgatt aatggttcgg ttgaagaaga ggacccttac 3241 caaggcacca ttgtgctggc cactgttaaa ggggacgtgc atgacatcgg caagaacata 3301 gttggcgtgg tgctggcctg taacaatttc agagttattg atttaggagt catgactccc 3361 tgtgataaga tacttcaagc tgctctggac cacaaagcag atataattgg cttgtcagga 3421 ctcatcactc catccttgga tgagatgatt tttgttgcca aggaaatgga gaggttggct 3481 ataaagattc cattgttgat aggaggagca accacttcaa gaacccacac agcagttaaa 3541 attgcaccac ggtacagcgc gcctgtgatc cacgtcctag atgcatccaa gagtgtggtg 3601 gtgtgttctc agctgttaga tgaaaatctg agagatgact actttgaaga aatactggaa 3661 gagtatgaag atattagaca ggaccactat gagtctctca aagagagaaa atacgtaccc 3721 ctaagtcaag ccagaaaaca cggtttccac attgattggc tctctgaacc tcatccagtg 3781 aagcccacgt ttattgggac ccaggtcttt gaggactaca acctgcaaaa gctggtggac 3841 tacattgact ggaagccttt ctttgacgtc tggcagctcc ggggcaagta cccgaaccga 3901 ggatttccca agatatttaa tgacaaagca gtaggtgaag aagccagaaa ggtatacaat 3961 gatgctcaga atatgctgaa catactgatt agtcaaaaga aactgcaggc caggggtgtg 4021 gttggattct ggccagcaca gagtgtccag gatgacatcc acctgtatgc agagggggtg 4081 gtgccccagg ccgctgagcc catagccacc ttctatggac tgaggcagca ggctgagaag 4141 gactcttcta gtacagaccc ctaccactgc ctctcagact tcattgctcc tttgcattct 4201 ggtgtctgtg actacttggg tctgtttgct gttgcctgct ttggggttga agagctgagt 4261 aagacctatg aggatgatgg cgatgactac agcagcatca tggtgaaggc gctgggcgac 4321 aggctggcag aggcctttgc agaggagctc catgagagag ttcgccgaga actgtgggcc 4381 tactctagga gtgagcagtt gggcgtccca gacttgcgca gactccggta tgagggcatc 4441 cggccagctc ctggttaccc cagccagcct gaccatactg agaagcttac catgtggaga 4501 ctggccagca tcgagcaggc cacaggcatc aggctgacag aatccttggc aatggcaccc 4561 gcttcagcag tgtcagggct gtacttctcc aacgtaaagg ccaaatattt tgctgtgggg 4621 aaaatttcca aagatcagac tgaggattat gctttgagga agaacatgcc agtggccgag 4681 gtggagaaat ggcttggtcc cattctgggc tatgacacag acCTCGAGGA CTACAAAGAC 4741 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGTAGTGA 4801 GGGCCCGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT 4861 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC 4921 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT 4981 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT 5041 GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC 5101 CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 5161 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 5221 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 5281 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 5341 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 5401 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 5461 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 5521 AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC 5581 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT 5641 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA 5701 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC 5761 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG 5821 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC 5881 CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA 5941 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG 6001 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG 6061 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC 6121 AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC 6181 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG 6241 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC 6301 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA 6361 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG 6421 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG 6481 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG 6541 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA 6601 TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC 6661 TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG 6721 ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT 6781 GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT 6841 TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC 6901 CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA 6961 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA 7021 TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC 7081 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 7141 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 7201 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 7261 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA 7321 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 7381 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 7441 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 7501 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 7561 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 7621 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 7681 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 7741 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 7801 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 7861 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 7921 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 7981 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT 8041 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 8101 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 8161 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 8221 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 8281 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 8341 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 8401 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 8461 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 8521 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 8581 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 8641 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 8701 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 8761 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 8821 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 8881 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 8941 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 9001 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 9061 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 9121 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 9181 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.