pLV2-CMV-IRAK1-EGFP-mCherry pl

pLV2-CMV-IRAK1-EGFP-mCherry plasmid

PVT108785

pLV2-CMV-IRAK1-EGFP-mCherry plasmid Information

Plasmid name: pLV2-CMV-IRAK1(human)-EGFP-mCherry
Alias: NM_001569.4;IRAK; pelle
Gene lenght: 2136
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red,Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-IRAK1-EGFP-mCherry plasmid Description

pLV2-CMV-IRAK1-EGFP-mCherry It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-IRAK1-EGFP-mCherry plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccATGGCC 2821 GGGGGGCCGG GCCCGGGGGA GCCCGCAGCC CCCGGCGCCC AGCACTTCTT GTACGAGGTG 2881 CCGCCCTGGG TCATGTGCCG CTTCTACAAA GTGATGGACG CCCTGGAGCC CGCCGACTGG 2941 TGCCAGTTCG CCGCCCTGAT CGTGCGCGAC CAGACCGAGC TGCGGCTGTG CGAGCGCTCC 3001 GGGCAGCGCA CGGCCAGCGT CCTGTGGCCC TGGATCAACC GCAACGCCCG TGTGGCCGAC 3061 CTCGTGCACA TCCTCACGCA CCTGCAGCTG CTCCGTGCGC GGGACATCAT CACAGCCTGG 3121 CACCCTCCCG CCCCGCTTCC GTCCCCAGGC ACCACTGCCC CGAGGCCCAG CAGCATCCCT 3181 GCACCCGCCG AGGCCGAGGC CTGGAGCCCC CGGAAGTTGC CATCCTCAGC CTCCACCTTC 3241 CTCTCCCCAG CTTTTCCAGG CTCCCAGACC CATTCAGGGC CTGAGCTCGG CCTGGTCCCA 3301 AGCCCTGCTT CCCTGTGGCC TCCACCGCCA TCTCCAGCCC CTTCTTCTAC CAAGCCAGGC 3361 CCAGAGAGCT CAGTGTCCCT CCTGCAGGGA GCCCGCCCCT TTCCGTTTTG CTGGCCCCTC 3421 TGTGAGATTT CCCGGGGCAC CCACAACTTC TCGGAGGAGC TCAAGATCGG GGAGGGTGGC 3481 TTTGGGTGCG TGTACCGGGC GGTGATGAGG AACACGGTGT ATGCTGTGAA GAGGCTGAAG 3541 GAGAACGCTG ACCTGGAGTG GACTGCAGTG AAGCAGAGCT TCCTGACCGA GGTGGAGCAG 3601 CTGTCCAGGT TTCGTCACCC AAACATTGTG GACTTTGCTG GCTACTGTGC TCAGAACGGC 3661 TTCTACTGCC TGGTGTACGG CTTCCTGCCC AACGGCTCCC TGGAGGACCG TCTCCACTGC 3721 CAGACCCAGG CCTGCCCACC TCTCTCCTGG CCTCAGCGAC TGGACATCCT TCTGGGTACA 3781 GCCCGGGCAA TTCAGTTTCT ACATCAGGAC AGCCCCAGCC TCATCCATGG AGACATCAAG 3841 AGTTCCAACG TCCTTCTGGA TGAGAGGCTG ACACCCAAGC TGGGAGACTT TGGCCTGGCC 3901 CGGTTCAGCC GCTTTGCCGG GTCCAGCCCC AGCCAGAGCA GCATGGTGGC CCGGACACAG 3961 ACAGTGCGGG GCACCCTGGC CTACCTGCCC GAGGAGTACA TCAAGACGGG AAGGCTGGCT 4021 GTGGACACGG ACACCTTCAG CTTTGGGGTG GTAGTGCTAG AGACCTTGGC TGGTCAGAGG 4081 GCTGTGAAGA CGCACGGTGC CAGGACCAAG TATCTGAAAG ACCTGGTGGA AGAGGAGGCT 4141 GAGGAGGCTG GAGTGGCTTT GAGAAGCACC CAGAGCACAC TGCAAGCAGG TCTGGCTGCA 4201 GATGCCTGGG CTGCTCCCAT CGCCATGCAG ATCTACAAGA AGCACCTGGA CCCCAGGCCC 4261 GGGCCCTGCC CACCTGAGCT GGGCCTGGGC CTGGGCCAGC TGGCCTGCTG CTGCCTGCAC 4321 CGCCGGGCCA AAAGGAGGCC TCCTATGACC CAGGTGTACG AGAGGCTAGA GAAGCTGCAG 4381 GCAGTGGTGG CGGGGGTGCC CGGGCATTCG GAGGCCGCCA GCTGCATCCC CCCTTCCCCG 4441 CAGGAGAACT CCTACGTGTC CAGCACTGGC AGAGCCCACA GTGGGGCTGC TCCATGGCAG 4501 CCCCTGGCAG CGCCATCAGG AGCCAGTGCC CAGGCAGCAG AGCAGCTGCA GAGAGGCCCC 4561 AACCAGCCCG TGGAGAGTGA CGAGAGCCTA GGCGGCCTCT CTGCTGCCCT GCGCTCCTGG 4621 CACTTGACTC CAAGCTGCCC TCTGGACCCA GCACCCCTCA GGGAGGCCGG CTGTCCTCAG 4681 GGGGACACGG CAGGAGAATC GAGCTGGGGG AGTGGCCCAG GATCCCGGCC CACAGCCGTG 4741 GAAGGACTGG CCCTTGGCAG CTCTGCATCA TCGTCGTCAG AGCCACCGCA GATTATCATC 4801 AACCCTGCCC GACAGAAGAT GGTCCAGAAG CTGGCCCTGT ACGAGGATGG GGCCCTGGAC 4861 AGCCTGCAGC TGCTGTCGTC CAGCTCCCTC CCAGGCTTGG GCCTGGAACA GGACAGGCAG 4921 GGGCCCGAAG AAAGTGATGA ATTTCAGAGC ggcggtggag gcagtggtgg aggcggtagc 4981 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 5041 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 5101 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 5161 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 5221 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 5281 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 5341 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 5401 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 5461 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 5521 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 5581 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 5641 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa 5701 ggatcccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa 5761 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg 5821 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc 5881 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct 5941 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac 6001 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc 6061 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctcac ctcaagcgta 6121 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg 6181 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga 6241 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggtgag caagggcgag 6301 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 6361 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 6421 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 6481 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 6541 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 6601 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag 6661 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 6721 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 6781 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 6841 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc 6901 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac 6961 tccaccggcg gcatggacga gctgtacaag tgaacgcgtc tggaacaatc aacctctgga 7021 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 7081 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 7141 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 7201 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 7261 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 7321 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 7381 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 7441 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 7501 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 7561 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 7621 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 7681 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 7741 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 7801 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 7861 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 7921 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 7981 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 8041 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 8101 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 8161 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 8221 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 8281 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 8341 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 8401 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 8461 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 8521 gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 8581 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 8641 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 8701 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 8761 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 8821 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 8881 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 8941 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 9001 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 9061 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 9121 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 9181 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 9241 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 9301 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 9361 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 9421 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 9481 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 9541 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 9601 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 9661 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 9721 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 9781 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 9841 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 9901 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 9961 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 10021 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 10081 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 10141 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 10201 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 10261 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 10321 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 10381 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 10441 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 10501 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 10561 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 10621 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 10681 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact 10741 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 10801 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 10861 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat 10921 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg 10981 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.