pTSBX-CMV-MCS-3×FLAG-EF1a-CopG

pTSBX-CMV-MCS-3×FLAG-EF1a-CopGFP-Puro plasmid

PVT108338

pTSBX-CMV-MCS-3×FLAG-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pTSBX-CMV-MCS-3×FLAG-EF1a-CopGFP-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pTSBX-CMV-MCS-3×FLAG-EF1a-CopGFP-Puro plasmid Description

pTSBX-CMV-MCS-3×FLAG-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pTSBX-CMV-MCS-3×FLAG-EF1a-CopGFP-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcac tagtgcggcc 2821 gcaagctttc tagaggatcc gactacaaag accatgacgg tgattataaa gatcatgaca 2881 tcgattacaa ggatgacgat gacaagtgac tcgaggcgat cgctccggtg cccgtcagtg 2941 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac 3001 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg 3061 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct 3121 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc 3181 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc 3241 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag 3301 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc 3361 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg 3421 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga 3481 gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga 3541 gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat 3601 gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta 3661 cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat 3721 caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca 3781 cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg 3841 caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac 3901 cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt 3961 cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag 4021 cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga 4081 ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc 4141 catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg 4201 acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga 4261 ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 4321 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 4381 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 4441 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 4501 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 4561 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 4621 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 4681 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 4741 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 4801 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 4861 caagcccggt gcctgaacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa 4921 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 4981 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 5041 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 5101 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 5161 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 5221 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 5281 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 5341 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 5401 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 5461 tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc 5521 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga 5581 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa 5641 ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca 5701 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc 5761 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg 5821 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac 5881 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga 5941 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta 6001 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg 6061 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc 6121 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 6181 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 6241 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 6301 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 6361 caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta ccgtcgacct 6421 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 6481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 6541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 6601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 6661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 6721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 6781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 6841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 6901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 6961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 7021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 7081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 7141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 7201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 7261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 7321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 7381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 7441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 7501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 7561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 7621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 7681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 7741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 7801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 7861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 7921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 7981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 8041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 8101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 8161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 8221 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 8281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 8341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 8401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 8461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 8521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 8581 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat 8641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 8701 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg 8761 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc 8821 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 8881 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt 8941 gttaaatatg tactacaaac ttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.