pTSBX-CMV-PPP6R3-3×FLAG-EF1a-C

pTSBX-CMV-PPP6R3-3×FLAG-EF1a-CopGFP-Puro plasmid

PVT109263

pTSBX-CMV-PPP6R3-3×FLAG-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pTSBX-CMV-PPP6R3(human)-3×FLAG-EF1a-CopGFP-Puro
Alias: NM_001164162.2;C11orf23; PP6R3; SAP190; SAPL; SAPLa;SAPS3
Gene lenght: 2601
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pTSBX-CMV-PPP6R3-3×FLAG-EF1a-CopGFP-Puro plasmid Description

pTSBX-CMV-PPP6R3-3×FLAG-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pTSBX-CMV-PPP6R3-3×FLAG-EF1a-CopGFP-Puro plasmid Sequence

1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA 61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC 121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA 181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG 241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG 301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG 361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT 421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG 661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG 721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA 781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG 841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG 901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT 961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT 1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA 1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC 1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG 1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC 1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG 1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC 1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC 1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT 1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA 1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA 1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT 1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT 1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA 1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT 1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT 1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA 1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA 2221 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 2281 TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 2341 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 2401 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 2461 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 2521 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 2581 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 2641 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 2701 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 2761 TCCATAGAAG ACACCGACTC TACTAGAGGA TCTATTTCCG GTGAATTCGC CACCatgttt 2821 tggaaatttg atcttcactc atcatcccac atagacacac ttctagaaag agaagatgta 2881 acactgaagg agttaatgga tgaggaagat gttttacagg aatgtaaagc tcagaaccgc 2941 aaacttatag agtttctgtt aaaagcagaa tgtctcgaag atttagtctc attcattata 3001 gaagaaccac ctcaagacat ggatgaaaag atcagataca agtatccaaa tatatcttgt 3061 gagttgctca cttctgatgt ctcccagatg aatgatagac tgggagaaga tgaatccttg 3121 ctaatgaaat tatatagctt cctcctaaac gattcccctt tgaatccact acttgccagt 3181 ttcttcagca aggtgctaag tattcttatc agcagaaaac cagaacagat tgtggatttc 3241 ttaaagaaga agcatgattt tgtagacctt attataaagc acataggaac ttctgctatc 3301 atggatttgt tgctcaggct cctgacgtgt atcgaacctc cacagcccag gcaagatgtg 3361 ctgaattggt taaatgagga gaaaattatc cagaggcttg tggaaatagt tcatccatcg 3421 caagaagaag atcgacattc aaatgcatca caatcacttt gtgaaattgt tcgcctgagc 3481 agagaccaga tgttacaaat tcagaacagt acagagcccg accccctgct tgccactcta 3541 gaaaagcaag aaattataga gcagcttcta tcaaatattt tccacaagga gaaaaatgag 3601 tcagccatag tcagtgcaat ccagatattg ctgactttac ttgagacacg acgaccaaca 3661 tttgaaggcc atatagagat ctgcccacca ggcatgagcc attcagcttg ttcagtaaac 3721 aagagtgttc tagaagccat cagaggaaga cttggatctt ttcatgaact cctgctggag 3781 ccacccaaga aaagtgtgat gaagaccaca tggggtgtgc tggatcctcc tgtggggaat 3841 acccggttga atgtcattag gttgatatcc agcctgcttc aaaccaatac cagcagtata 3901 aatggggacc ttatggagct gaatagcatt ggagtcatat tgaacatgtt cttcaagtat 3961 acatggaata actttttgca tacacaagtg gaaatttgta ttgcactgat tcttgcaagt 4021 ccttttgaaa acacagaaaa tgccacaatt accgatcaag actccactgg tgataatttg 4081 ttattaaaac atcttttcca aaaatgtcaa ttaatagaac gaatacttga agcctgggaa 4141 atgaatgaga agaaacaggc tgagggagga agacggcatg gttacatggg acacctaacg 4201 aggatagcta actgtatcgt gcacagcact gacaagggcc ccaacagtgc attagtgcag 4261 cagcttatca aagatcttcc cgacgaagtc agggaacgat gggagacgtt ctgcacaagc 4321 tccttaggag aaactaacaa gaggaacacg gtagatctag ttacaacctg ccatattcat 4381 tcatccagtg atgatgaaat tgactttaaa gaaacgggtt tctcacagga ttcttctttg 4441 cagcaaatgc aacaaatgac gtccaatttt attgaccagt ttggcttcaa cgatgagaag 4501 tttgcagatc aagatgacat tggcaatgtt tcttttgatc gagtatcaga catcaacttt 4561 actctcaata caaatgaaag tggaaatatt gccttgtttg aagcatgttg taaggaaaga 4621 atacaacagt ttgatgatgg tggctctgat gaggaagata tatgggagga aaagcacatc 4681 gcattcacac cagaatccca aagacgatcc agctcgggga gtacagacag tgaggaaagt 4741 acagactctg aagaagaaga tggagcaaag caagacttgt ttgaacccag cagtgccaac 4801 acggaggata aaatggaggt ggacctgagt gaaccaccca actggtcagc taactttgat 4861 gtcccaatgg aaacaaccca cggtgctcca ttggattctg tgggatctga tgtctggagc 4921 acagaggagc cgatgccaac taaagagacg ggctgggctt ctttttcaga gttcacgtct 4981 tccctgagca caaaagattc tttaaggagt aattctccag tggaaatgga aaccagcact 5041 gaacccatgg accctctgac tcccagtgcg gctgccctgg cagtgcagcc agaagcggca 5101 ggcagtgtgg ccatggaagc cagctctgac ggagaggagg atgcagaaag tacagacaag 5161 gtaactgaga cagtgatgaa tggcggcatg aaggaaacgc tcagcctcac tgtagatgcc 5221 aagacagaga ctgcggtctt caaaagtgag gaagggaaac tgtctacctc tcaagatgct 5281 gcttgtaaag acgcagagga gtgtcccgag actgcagagg cgaagtgcgc ggcgcccagg 5341 cctcccagca gcagtcccga gcagaggact ggccaaccaa gcgcaccagg tgacacttca 5401 gtgaatggcc ctgtaGGATC CGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 5461 ATCGACTACA AGGATGACGA TGACAAGTAG CTCGAGGCGA TCGCTCCGGT GCCCGTCAGT 5521 GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA 5581 CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC 5641 GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC 5701 TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC 5761 CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC 5821 CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA 5881 GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT 5941 CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC 6001 GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACGCTAGA CGCCACCATG GAGAGCGACG 6061 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 6121 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCAAGCA GGGCCGCATG ACCAACAAGA 6181 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 6241 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 6301 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 6361 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGGTGG 6421 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 6481 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGTGCTGGT GGGCAGCTTC GCCCGCACCT 6541 TCAGCCTGCG CGACGGCGGC TACTACAGCT TCGTGGTGGA CAGCCACATG CACTTCAAGA 6601 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 6661 AGGAGCTGCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 6721 CCATCGCCTT CGCCAGATCC CGCGCTCAGT CGTCCAATTC TGCCGTGGAC GGCACCGCCG 6781 GACCCGGCTC CACCGGATCT CGCGAGGGCA GAGGAAGTCT TCTAACATGC GGTGACGTGG 6841 AGGAGAATCC CGGCCCTATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG 6901 ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC 6961 ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA 7021 CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG 7081 TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA 7141 TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC 7201 CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC 7261 AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG 7321 GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG 7381 GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC 7441 GCAAGCCCGG TGCCTGAACG CGTCTGGAAC AATCAACCTC TGGATTACAA AATTTGTGAA 7501 AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA 7561 ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA 7621 TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG 7681 TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC 7741 CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC 7801 CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG 7861 GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG 7921 ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG 7981 CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC 8041 CTTTGGGCCG CCTCCCCGCC TGGAATTAAT TCTGCAGTCG AGACCTAGAA AAACATGGAG 8101 CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG TGCCTGGCTA GAAGCACAAG 8161 AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA ATGACTTACA 8221 AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGAG GGGACTGGAA GGGCTAATTC 8281 ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA GGCTACTTCC 8341 CTGATTAGCA GAACTACACA CCAGGGCCAG GGGTCAGATA TCCACTGACC TTTGGATGGT 8401 GCTACAAGCT AGTACCAGTT GAGCCAGATA AGGTAGAAGA GGCCAATAAA GGAGAGAACA 8461 CCAGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA GAAGTGTTAG 8521 AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACGTGGC CCGAGAGCTG CATCCGGAGT 8581 ACTTCAAGAA CTGCTGATAT CGAGCTTGCT ACAAGGGACT TTCCGCTGGG GACTTTCCAG 8641 GGAGGCGTGG CCTGGGCGGG ACTGGGGAGT GGCGAGCCCT CAGATCCTGC ATATAAGCAG 8701 CTGCTTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT 8761 GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA 8821 GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA 8881 GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT 8941 GCAAAGAAAT GAATATCAGA GAGTGAGAGG CCTTGACATT GCTAGCGTTT ACCGTCGACC 9001 TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 9061 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA 9121 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC 9181 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT 9241 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA 9301 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 9361 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 9421 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 9481 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 9541 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 9601 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 9661 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 9721 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 9781 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 9841 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG 9901 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 9961 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 10021 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 10081 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 10141 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 10201 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 10261 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 10321 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 10381 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 10441 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 10501 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 10561 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 10621 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 10681 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 10741 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 10801 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 10861 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 10921 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 10981 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 11041 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 11101 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 11161 CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC AACTTGTTTA TTGCAGCTTA 11221 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 11281 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCAACTG 11341 GATAACTCAA GCTAACCAAA ATCATCCCAA ACTTCCCACC CCATACCCTA TTACCACTGC 11401 CAATTACCTG TGGTTTCATT TACTCTAAAC CTGTGATTCC TCTGAATTAT TTTCATTTTA 11461 AAGAAATTGT ATTTGTTAAA TATGTACTAC AAACTTAGTA GTTTTTAAAG AAATTGTATT 11521 TGTTAAATAT GTACTACAAA CTTAGTAGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.