pT7-6×His-SUMO-PLSCR1-6×His pl

pT7-6×His-SUMO-PLSCR1-6×His plasmid

PVT108313

pT7-6×His-SUMO-PLSCR1-6×His plasmid Information

Plasmid name: pT7-6×His-SUMO-PLSCR1(human)-6×His
Alias: NM_021105.3;MMTRA1B
Gene lenght: 954
Host: E.coli
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: SUMO-F
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pT7-6×His-SUMO-PLSCR1-6×His plasmid Description

pT7-6×His-SUMO-PLSCR1-6×His is a plasmid suitable for protein expression in Escherichia coli.

pT7-6×His-SUMO-PLSCR1-6×His plasmid Sequence

1 CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA 61 ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT 121 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA 181 GAGGATCGAG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG 241 GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATGG 301 GCAGCAGCCA TCATCATCAT CATCACGGCA GCGGCCTGGT GCCGCGCGGC AGCGCTAGCA 361 TGTCGGACTC AGAAGTCAAT CAAGAAGCTA AGCCAGAGGT CAAGCCAGAA GTCAAGCCTG 421 AGACTCACAT CAATTTAAAG GTGTCCGATG GATCTTCAGA GATCTTCTTC AAGATCAAAA 481 AGACCACTCC TTTAAGAAGG CTGATGGAAG CGTTCGCTAA AAGACAGGGT AAGGAAATGG 541 ACTCCTTAAG ATTCTTGTAC GACGGTATTA GAATTCAAGC TGATCAGACC CCTGAAGATT 601 TGGACATGGA GGATAACGAT ATTATTGAGG CTCACAGAGA ACAGATTGGT GGTAGACAAG 661 CTatggacaa acaaaactca cagatgaatg cttctcaccc ggaaacaaac ttgccagttg 721 ggtatcctcc tcagtatcca ccgacagcat tccaaggacc tccaggatat agtggctacc 781 ctgggcccca ggtcagctac ccacccccac cagccggcca ttcaggtcct ggcccagctg 841 gctttcctgt cccaaatcag ccagtgtata atcagccagt atataatcag ccagttggag 901 ctgcaggggt accatggatg ccagcgccac agcctccatt aaactgtcca cctggattag 961 aatatttaag tcagatagat cagatactga ttcatcagca aattgaactt ctggaagttt 1021 taacaggttt tgaaactaat aacaaatatg aaattaagaa cagctttgga cagagggttt 1081 actttgcagc ggaagatact gattgctgta cccgaaattg ctgtgggcca tctagacctt 1141 ttaccttgag gattattgat aatatgggtc aagaagtcat aactctggag agaccactaa 1201 gatgtagcag ctgttgttgt ccctgctgcc ttcaggagat agaaatccaa gctcctcctg 1261 gtgtaccaat aggttatgtt attcagactt ggcacccatg tctaccaaag tttacaattc 1321 aaaatgagaa aagagaggat gtactaaaaa taagtggtcc atgtgttgtg tgcagctgtt 1381 gtggagatgt tgattttgag attaaatctc ttgatgaaca gtgtgtggtt ggcaaaattt 1441 ccaagcactg gactggaatt ttgagagagg catttacaga cgctgataac tttggaatcc 1501 agttcccttt agaccttgat gttaaaatga aagctgtaat gattggtgcc tgtttcctca 1561 ttgacttcat gttttttgaa agcactggca gccaggaaca aaaatcagga gtgtggcTCG 1621 AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT 1681 TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT 1741 TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATT GGCGAATGGG ACGCGCCCTG 1801 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC 1861 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 1921 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG 1981 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG 2041 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT 2101 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 2161 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT 2221 TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 2281 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAA TTAATTCTTA 2341 GAAAAACTCA TCGAGCATCA AATGAAACTG CAATTTATTC ATATCAGGAT TATCAATACC 2401 ATATTTTTGA AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC AGTTCCATAG 2461 GATGGCAAGA TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA TACAACCTAT 2521 TAATTTCCCC TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG TGACGACTGA 2581 ATCCGGTGAG AATGGCAAAA GTTTATGCAT TTCTTTCCAG ACTTGTTCAA CAGGCCAGCC 2641 ATTACGCTCG TCATCAAAAT CACTCGCATC AACCAAACCG TTATTCATTC GTGATTGCGC 2701 CTGAGCGAGA CGAAATACGC GATCGCTGTT AAAAGGACAA TTACAAACAG GAATCGAATG 2761 CAACCGGCGC AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT CAGGATATTC 2821 TTCTAATACC TGGAATGCTG TTTTCCCGGG GATCGCAGTG GTGAGTAACC ATGCATCATC 2881 AGGAGTACGG ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA GCCAGTTTAG 2941 TCTGACCATC TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT TCAGAAACAA 3001 CTCTGGCGCA TCGGGCTTCC CATACAATCG ATAGATTGTC GCACCTGATT GCCCGACATT 3061 ATCGCGAGCC CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA ATCGCGGCCT 3121 AGAGCAAGAC GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC TGTTTATGTA 3181 AGCAGACAGT TTTATTGTTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 3241 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 3301 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 3361 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 3421 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 3481 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 3541 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 3601 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 3661 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 3721 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 3781 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 3841 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 3901 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 3961 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 4021 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT 4081 GCGGTATTTC ACACCGCAAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT 4141 AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG 4201 CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA 4261 GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC 4321 GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC 4381 TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA 4441 AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG 4501 GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG 4561 GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA 4621 TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA 4681 GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG 4741 GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT 4801 CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT 4861 ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC 4921 GACAGGAGCA CGATCATGCG CACCCGTGGG GCCGCCATGC CGGCGATAAT GGCCTGCTTC 4981 TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT 5041 CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG 5101 AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC 5161 ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG 5221 GCTCTCAAGG GCATCGGTCG AGATCCCGGT GCCTAATGAG TGAGCTAACT TACATTAATT 5281 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA 5341 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCCAGGGTGG TTTTTCTTTT 5401 CACCAGTGAG ACGGGCAACA GCTGATTGCC CTTCACCGCC TGGCCCTGAG AGAGTTGCAG 5461 CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC TGTTTGATGG TGGTTAACGG 5521 CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC ACTACCGAGA TATCCGCACC 5581 AACGCGCAGC CCGGACTCGG TAATGGCGCG CATTGCGCCC AGCGCCATCT GATCGTTGGC 5641 AACCAGCATC GCAGTGGGAA CGATGCCCTC ATTCAGCATT TGCATGGTTT GTTGAAAACC 5701 GGACATGGCA CTCCAGTCGC CTTCCCGTTC CGCTATCGGC TGAATTTGAT TGCGAGTGAG 5761 ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA GAACTTAATG GGCCCGCTAA 5821 CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC ACGCCCAGTC GCGTACCGTC 5881 TTCATGGGAG AAAATAATAC TGTTGATGGG TGTCTGGTCA GAGACATCAA GAAATAACGC 5941 CGGAACATTA GTGCAGGCAG CTTCCACAGC AATGGCATCC TGGTCATCCA GCGGATAGTT 6001 AATGATCAGC CCACTGACGC GTTGCGCGAG AAGATTGTGC ACCGCCGCTT TACAGGCTTC 6061 GACGCCGCTT CGTTCTACCA TCGACACCAC CACGCTGGCA CCCAGTTGAT CGGCGCGAGA 6121 TTTAATCGCC GCGACAATTT GCGACGGCGC GTGCAGGGCC AGACTGGAGG TGGCAACGCC 6181 AATCAGCAAC GACTGTTTGC CCGCCAGTTG TTGTGCCACG CGGTTGGGAA TGTAATTCAG 6241 CTCCGCCATC GCCGCTTCCA CTTTTTCCCG CGTTTTCGCA GAAACGTGGC TGGCCTGGTT 6301 CACCACGCGG GAAACGGTCT GATAAGAGAC ACCGGCATAC TCTGCGACAT CGTATAACGT 6361 TACTGGTTTC ACATTCACCA CCCTGAATTG ACTCTCTTCC GGGCGCTATC ATGCCATACC 6421 GCGAAAGGTT TTGCGCCATT CGATGGTGTC CGGGATCTCG ACGCTCTCCC TTATGCGACT 6481 CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA 6541 ATGGTGCATG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.