pLV3-CMV-Gorab-6×His-EF1a-CopG

pLV3-CMV-Gorab-6×His-EF1a-CopGFP-Puro plasmid

PVT108095

pLV3-CMV-Gorab-6×His-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Gorab(mouse)-6×His-EF1a-CopGFP-Puro
Alias: NM_178883.6
Gene lenght: 1104
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Gorab-6×His-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-Gorab-6×His-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Gorab-6×His-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagaGCCACC atggctcagg attgggcggg cttctctgag gaagagctga ggagactaaa 2341 gcagaataaa gatccatttg aacctcagcg tcgaattcct gtgaagaaaa ctcgacaaca 2401 gcttcagcgt gaaaaagccc ttctagagca gagccaaaag cttggcctcc aagatgggtc 2461 agcctcattg cttccagagc agctgctctc tgcacccaaa cagagagcta acagtcaaaa 2521 gccacgctct ccttcccctg tggcccccag tcccctcaca cccacctctt cctctggcga 2581 tggaaagcta cctggtgttg gaagtcagcc ccaagaacca ggacttgaga attcccacca 2641 tggtcacaaa agtgccgagg ttcgagctcc aaagccagat tgcaaagtgg agaaaaagaa 2701 aatggaattg caagagaaat ctcgttggga agtcctccaa caagaacagc gactaatgga 2761 agagaaaaat aaacgtaaga aagccctttt ggctcaagcc attgcggaaa gatctaagaa 2821 gacccaagca gagaccataa aactaaagag gatccagaag gagttgcagg ccttagacga 2881 catggtgtca gctgacattg gcatcctcag gaacagaatc gaccaggcca gcctggagta 2941 ttcctacgca cggaagcgct ttgatagagc tgaagctgag tacattacag caaagctgga 3001 tttacagcgc aaaaccgaga cgaaagagca gctcaccgaa cacctctgca ccatcatcca 3061 gcagaacgag ctccgaaagg ccaagaagct ggaggagctg atgcagcagc tggacgtgca 3121 ggctgatgag gaggctttgc aactcgaggt ggaggttgaa cagttgcttc gggaacagga 3181 agcagaggca gccaaacaaa tggcttctgt agagaggctg tgtccgcctg atggcgagag 3241 tgtgtcttca gaactggctg aagagaacaa tgagcctcaa aagcaagctc cttccccaga 3301 gacagacaag ccaggcaaat gttgcagtag ctctccccat agactagact gcccagatcc 3361 aggagccaag aacttttcag ctgctgtggc cacaggatcc CACCACCACC ACCACCACta 3421 gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc 3481 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg 3541 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg 3601 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc 3661 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc 3721 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc 3781 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg 3841 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct 3901 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct 3961 gtgaccggcg cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat 4021 ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg 4081 cggagagggc acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc 4141 cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg 4201 cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac 4261 caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg 4321 ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga 4381 cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc 4441 catgggcgat aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg 4501 ctactacagc ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat 4561 cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac 4621 cgagctgggc atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc 4681 ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc 4741 tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat 4801 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 4861 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 4921 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 4981 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 5041 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 5101 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 5161 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 5221 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 5281 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 5341 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa 5401 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 5461 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 5521 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 5581 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 5641 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 5701 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 5761 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 5821 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 5881 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg 5941 ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag 6001 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 6061 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct 6121 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6181 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 6241 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6301 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6361 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 6421 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 6481 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct 6541 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 6601 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc 6661 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 6721 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 6781 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 6841 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 6901 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 6961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 7021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 7081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 7141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 7201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 7261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 7321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 7381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 7441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 7501 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 7561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 7621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 7681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 7741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 7801 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 7861 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 7921 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 7981 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 8041 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 8101 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 8161 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 8221 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 8281 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 8341 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 8401 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 8461 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 8521 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 8581 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 8641 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 8701 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 8761 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 8821 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 8881 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 8941 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 9001 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 9061 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 9121 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 9181 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 9241 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag 9301 ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.