pBiFC-CMV-FLAG-Stat1-VN173 pla

pBiFC-CMV-FLAG-Stat1-VN173 plasmid

PVT108080

pBiFC-CMV-FLAG-Stat1-VN173 plasmid Information

Plasmid name: pBiFC-CMV-FLAG-Stat1(mouse)-VN173
Alias: 2010005J02Rik
Gene lenght: 2265
Host: Mammalian cells
Use(s): Signal report,Bimolecular fluorescence
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pBiFC-CMV-FLAG-Stat1-VN173 plasmid Description

pBiFC-CMV-FLAG-Stat1-VN173 This is a plasmid suitable for bimolecular fluorescence in mammalian cells.

pBiFC-CMV-FLAG-Stat1-VN173 plasmid Sequence

1 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT 61 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG 121 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTGATCT ATACATTGAA 181 TCAATATTGG CAATTAGCCA TATTAGTCAT TGGTTATATA GCATAAATCA ATATTGGCTA 241 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC 301 AATATGACCG CCATGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG 361 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC 421 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 481 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC 541 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA 601 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG 661 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC 721 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 781 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC 841 CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC 901 TCGTTTAGTG AACCGTCAGA ATTGATCTAC CATGGACTAC AAAGACGATG ACGACAAGCT 961 Tatgtcacag tggttcgagc ttcagcagct ggactccaag ttcctggagc aggtccacca 1021 gctgtacgat gacagtttcc ccatggaaat cagacagtac ctggcccagt ggctggaaaa 1081 gcaagactgg gagcacgctg cctatgatgt ctcgtttgcg accatccgct tccatgacct 1141 cctctcacag ctggacgacc agtacagccg cttttctctg gagaataatt tcttgttgca 1201 gcacaacata cggaaaagca agcgtaatct ccaggataac ttccaagaag atcccgtaca 1261 gatgtccatg atcatctaca actgtctgaa ggaagaaagg aagattttgg aaaatgccca 1321 aagatttaat caggcccagg agggaaatat tcagaacact gtgatgttag ataaacagaa 1381 ggagctggac agtaaagtca gaaatgtgaa ggatcaagtc atgtgcatag agcaggaaat 1441 caagacccta gaagaattac aagatgaata tgactttaaa tgcaaaacct ctcagaacag 1501 agaaggtgaa gccaatggtg tggcgaagag cgaccaaaaa caggaacagc tgctgctcca 1561 caagatgttt ttaatgcttg acaataagag aaaggagata attcacaaaa tcagagagtt 1621 gctgaattcc atcgagctca ctcagaacac tctgattaat gacgagctcg tggagtggaa 1681 gcgaaggcag cagagcgcct gcatcggggg accgcccaac gcctgcctgg atcagctgca 1741 aagctggttc accattgttg cagagaccct gcagcagatc cgtcagcagc ttaaaaagct 1801 ggaggagttg gaacagaaat tcacctatga gcccgaccct attacaaaaa acaagcaggt 1861 gttgtcagat cgaaccttcc tcctcttcca gcagctcatt cagagctcct tcgtggtaga 1921 acgacagccg tgcatgccca ctcacccgca gaggcccctg gtcttgaaga ctggggtaca 1981 gttcactgtc aagctgagac tgttggtgaa attgcaagag ctgaactata acttgaaagt 2041 gaaagtctca tttgacaaag atgtgaacga gaaaaacaca gttaaaggat ttcggaagtt 2101 caacatcttg ggtacgcaca caaaagtgat gaacatggaa gaatccacca acggaagtct 2161 ggcagctgag ttccgacacc tgcaactgaa ggaacagaaa aacgctggga acagaactaa 2221 tgaggggcct ctcattgtca ccgaagaact tcactctctt agctttgaaa cccagttgtg 2281 ccagccaggc ttggtgattg acctggaggt ctttgttccc tttcagacca cctctcttcc 2341 tgtcgtggtg atctccaacg tcagccagct ccccagtggc tgggcgtcta tcctgtggta 2401 caacatgctg gtgacagagc ccaggaatct ctccttcttc ctgaaccccc cgtgcgcgtg 2461 gtggtcccag ctctcagagg tgttgagttg gcagttttca tcagtcacca agagaggtct 2521 gaacgcagac cagctgagca tgctgggaga gaagctgctg ggccctaatg ctggccctga 2581 tggtcttatt ccatggacaa ggttttgtaa ggaaaatatt aatgataaaa atttctcctt 2641 ctggccttgg attgacacca tcctagagct cattaagaag cacctgctgt gcctctggaa 2701 tgatgggtgc attatgggct tcatcagcaa ggagcgagaa cgcgctctgc tcaaggacca 2761 gcagccaggg acgttcctgc ttagattcag tgagagctcc cgggaagggg ccatcacatt 2821 cacatgggtg gaacggtccc agaacggagg tgaacctgac ttccatgccg tggagcccta 2881 cacgaaaaaa gaactttcag ctgttacttt cccagatatt attcgcaact acaaagtcat 2941 ggctgccgag aacataccag agaatcccct gaagtatctg taccccaata ttgacaaaga 3001 ccacgccttt gggaagtatt attccagacc aaaggaagca ccagaaccga tggagcttga 3061 cgaccctaag cgaactggat acatcaagac tgagttgatt tctgtgtctg aagtccaccc 3121 ttctagactt cagaccacag acaacctgct tcccatgtct ccagaggagt ttgatgagat 3181 gtcccggata gtgggccccg aatttgacag tatgatgagc acagtaggtg gaggcggatc 3241 aggaggcggt ggatctTCTA GAAGATCCAT CGCCACCATG GTGAGCAAGG GCGAGGAGCT 3301 GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT 3361 CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGCTGAT 3421 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG 3481 CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC 3541 CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA 3601 GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG 3661 CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG 3721 CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT 3781 CCGCCACAAC ATCGAGTAGG GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC 3841 TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT 3901 GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT 3961 GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA 4021 GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA 4081 TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT 4141 AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT 4201 CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA 4261 CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC 4321 AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG 4381 GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC 4441 CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 4501 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG 4561 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC 4621 CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC 4681 ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT 4741 TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCTCGAGG 4801 AACTGAAAAA CCAGAAAGTT AATTCCCTAT AGTGAGTCGT ATTAAATTCG TAATCATGTC 4861 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 4921 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 4981 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 5041 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA 5101 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 5161 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 5221 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 5281 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 5341 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 5401 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 5461 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 5521 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 5581 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 5641 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 5701 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 5761 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 5821 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 5881 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 5941 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 6001 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 6061 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 6121 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 6181 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 6241 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 6301 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 6361 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 6421 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 6481 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 6541 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 6601 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 6661 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 6721 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 6781 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 6841 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 6901 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 6961 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG 7021 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC 7081 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 7141 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG 7201 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG 7261 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT 7321 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 7381 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT 7441 TAACAAAATA TTAACGCTTA CAATTT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.