pCMV-RBBP4-3×FLAG-SP6-Neo plas

pCMV-RBBP4-3×FLAG-SP6-Neo plasmid

PVT108054

pCMV-RBBP4-3×FLAG-SP6-Neo plasmid Information

Plasmid name: pCMV-RBBP4(human)-3×FLAG-SP6-Neo
Alias: NM_005610.3;lin-53; NURF55; RBAP48
Gene lenght: 1275
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-RBBP4-3×FLAG-SP6-Neo plasmid Description

pCMV-RBBP4-3×FLAG-SP6-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-RBBP4-3×FLAG-SP6-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttGCCACC 901 atggccgaca aggaagcagc cttcgacgac gcagtggaag aacgagtgat caacgaggaa 961 tacaaaatat ggaaaaagaa cacccctttt ctttatgatt tggtgatgac ccatgctctg 1021 gagtggccca gcctaactgc ccagtggctt ccagatgtaa ccagaccaga agggaaagat 1081 ttcagcattc atcgacttgt cctggggaca cacacatcgg atgaacaaaa ccatcttgtt 1141 atagccagtg tgcagctccc taatgatgat gctcagtttg atgcgtcaca ctacgacagt 1201 gagaaaggag aatttggagg ttttggttca gttagtggaa aaattgaaat agaaatcaag 1261 atcaaccatg aaggagaagt aaacagggcc cgttatatgc cccagaaccc ttgtatcatc 1321 gcaacaaaga ctccttccag tgatgttctt gtttttgact atacaaaaca tccttctaaa 1381 ccagatcctt ctggagagtg caacccagac ttgcgtctcc gtggacatca gaaggaaggc 1441 tatgggcttt cttggaaccc aaatctcagt gggcacttac ttagtgcttc agatgaccat 1501 accatctgcc tgtgggacat cagtgccgtt ccaaaggagg gaaaagtggt agatgcgaag 1561 accatcttta cagggcatac ggcagtagta gaagatgttt cctggcatct actccatgag 1621 tctctgtttg ggtcagttgc tgatgatcag aaacttatga tttgggatac tcgttcaaac 1681 aatacttcca aaccaagcca ctcagttgat gctcacactg ctgaagtgaa ctgcctttct 1741 ttcaatcctt atagtgagtt cattcttgcc acaggatcag ctgacaagac tgttgccttg 1801 tgggatctga gaaatctgaa acttaagttg cattcctttg agtcacataa ggatgaaata 1861 ttccaggttc agtggtcacc tcacaatgag actattttag cttccagtgg tactgatcgc 1921 agactgaatg tctgggattt aagtaaaatt ggagaggaac aatccccaga agatgcagaa 1981 gacgggccac cagagttgtt gtttattcat ggtggtcata ctgccaagat atctgatttc 2041 tcctggaatc ccaatgaacc ttgggtgatt tgttctgtat cagaagacaa tatcatgcaa 2101 gtgtggcaaa tggcagagaa catttataat gatgaagacc ctgaaggaag cgtggatcca 2161 gaaggacaag ggtccggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 2221 ATCGACTACA AGGATGACGA TGACAAGtag ggatccacta gtaacggccg ccagtgtgct 2281 ggaattctgc agatatccat cacactggcg gccgctcgag catgcatcta gagggcccta 2341 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt 2401 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 2461 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 2521 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 2581 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 2641 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 2701 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 2761 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt 2821 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 2881 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 2941 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 3001 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga 3061 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 3121 gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3181 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 3241 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 3301 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 3361 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 3421 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 3481 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 3541 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 3601 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 3661 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 3721 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 3781 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 3841 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 3901 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 3961 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 4021 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 4081 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 4141 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 4201 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 4261 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 4321 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 4381 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 4441 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 4501 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 4561 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 4621 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 4681 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 4741 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 4801 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 4861 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 4921 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 4981 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 5041 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 5101 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 5161 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 5221 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 5281 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 5341 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 5401 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 5461 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 5521 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 5581 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 5641 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 5701 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 5761 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 5821 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 5881 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 5941 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 6001 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 6061 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 6121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 6181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 6241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 6301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 6361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 6421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 6481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 6541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 6601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 6661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 6721 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 6781 acctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.