pCMV-IRS1-3×FLAG-Neo plasmid

pCMV-IRS1-3×FLAG-Neo plasmid

PVT108053

pCMV-IRS1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-IRS1(human)-3×FLAG-Neo
Alias: NM_005544.3;HIRS-1
Gene lenght: 3726
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F(special construction)
Reverse primer:
Copy number: High
Induction:

pCMV-IRS1-3×FLAG-Neo plasmid Description

pCMV-IRS1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-IRS1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatggc gagccctccg gagagcgatg 781 gcttctcgga cgtgcgcaag gtgggctacc tgcgcaaacc caagagcatg cacaaacgct 841 tcttcgtact gcgcgcggcc agcgaggctg ggggcccggc gcgcctcgag tactacgaga 901 acgagaagaa gtggcggcac aagtcgagcg cccccaaacg ctcgatcccc cttgagagct 961 gcttcaacat caacaagcgg gctgactcca agaacaagca cctggtggct ctctacaccc 1021 gggacgagca ctttgccatc gcggcggaca gcgaggccga gcaagacagc tggtaccagg 1081 ctctcctaca gctgcacaac cgtgctaagg gccaccacga cggagctgcg gccctcgggg 1141 cgggaggtgg tgggggcagc tgcagcggca gctccggcct tggtgaggct ggggaggact 1201 tgagctacgg tgacgtgccc ccaggacccg cattcaaaga ggtctggcaa gtgatcctga 1261 agcccaaggg cctgggtcag acaaagaacc tgattggtat ctaccgcctt tgcctgacca 1321 gcaagaccat cagcttcgtg aagctgaact cggaggcagc ggccgtggtg ctgcagctga 1381 tgaacatcag gcgctgtggc cactcggaaa acttcttctt catcgaggtg ggccgttctg 1441 ccgtgacggg gcccggggag ttctggatgc aggtggatga ctctgtggtg gcccagaaca 1501 tgcacgagac catcctggag gccatgcggg ccatgagtga tgagttccgc cctcgcagca 1561 agagccagtc ctcgtccaac tgctctaacc ccatcagcgt ccccctgcgc cggcaccatc 1621 tcaacaatcc cccgcccagc caggtggggc tgacccgccg atcacgcact gagagcatca 1681 ccgccacctc cccggccagc atggtgggcg ggaagccagg ctccttccgt gtccgcgcct 1741 ccagtgacgg cgaaggcacc atgtcccgcc cagcctcggt ggacggcagc cctgtgagtc 1801 ccagcaccaa cagaacccac gcccaccggc atcggggcag cgcccggctg caccccccgc 1861 tcaaccacag ccgctccatc cccatgccgg cttcccgctg ctcgccttcg gccaccagcc 1921 cggtcagtct gtcgtccagt agcaccagtg gccatggctc cacctcggat tgtctcttcc 1981 cacggcgatc tagtgcttcg gtgtctggtt cccccagcga tggcggtttc atctcctcgg 2041 atgagtatgg ctccagtccc tgcgatttcc ggagttcctt ccgcagtgtc actccggatt 2101 ccctgggcca caccccacca gcccgcggtg aggaggagct aagcaactat atctgcatgg 2161 gtggcaaggg gccctccacc ctgaccgccc ccaacggtca ctacattttg tctcggggtg 2221 gcaatggcca ccgctgcacc ccaggaacag gcttgggcac gagtccagcc ttggctgggg 2281 atgaagcagc cagtgctgca gatctggata atcggttccg aaagagaact cactcggcag 2341 gcacatcccc taccattacc caccagaaga ccccgtccca gtcctcagtg gcttccattg 2401 aggagtacac agagatgatg cctgcctacc caccaggagg tggcagtgga ggccgactgc 2461 cgggacacag gcactccgcc ttcgtgccca cccgctccta cccagaggag ggtctggaaa 2521 tgcacccctt ggagcgtcgg ggggggcacc accgcccaga cagctccacc ctccacacgg 2581 atgatggcta catgcccatg tccccagggg tggccccagt gcccagtggc cgaaagggca 2641 gtggagacta tatgcccatg agccccaaga gcgtatctgc cccacagcag atcatcaatc 2701 ccatcagacg ccatccccag agagtggacc ccaatggcta catgatgatg tcccccagcg 2761 gtggctgctc tcctgacatt ggaggtggcc ccagcagcag cagcagcagc agcaacgccg 2821 tcccttccgg gaccagctat ggaaagctgt ggacaaacgg ggtagggggc caccactctc 2881 atgtcttgcc tcaccccaaa cccccagtgg agagcagcgg tggtaagctc ttaccttgca 2941 caggtgacta catgaacatg tcaccagtgg gggactccaa caccagcagc ccctccgact 3001 gctactacgg ccctgaggac ccccagcaca agccagtcct ctcctactac tcattgccaa 3061 gatcctttaa gcacacccag cgccccgggg agccggagga gggtgcccgg catcagcacc 3121 tccgcctttc cactagctct ggtcgccttc tctatgctgc aacagcagat gattcttcct 3181 cttccaccag cagcgacagc ctgggtgggg gatactgcgg ggctaggctg gagcccagcc 3241 ttccacatcc ccaccatcag gttctgcagc cccatctgcc tcgaaaggtg gacacagctg 3301 ctcagaccaa tagccgcctg gcccggccca cgaggctgtc cctgggggat cccaaggcca 3361 gcaccttacc tcgggcccga gagcagcagc agcagcagca gcccttgctg caccctccag 3421 agcccaagag cccgggggaa tatgtcaata ttgaatttgg gagtgatcag tctggctact 3481 tgtctggccc ggtggctttc cacagctcac cttctgtcag gtgtccatcc cagctccagc 3541 cagctcccag agaggaagag actggcactg aggagtacat gaagatggac ctggggccgg 3601 gccggagggc agcctggcag gagagcactg gggtcgagat gggcagactg ggccctgcac 3661 ctcccggggc tgctagcatt tgcaggccta cccgggcagt gcccagcagc cggggtgact 3721 acatgaccat gcagatgagt tgtccccgtc agagctacgt ggacacctcg ccagctgccc 3781 ctgtaagcta tgctgacatg cgaacaggca ttgctgcaga ggaggtgagc ctgcccaggg 3841 ccaccatggc tgctgcctcc tcatcctcag cagcctctgc ttccccgact gggcctcaag 3901 gggcagcaga gctggctgcc cactcgtccc tgctgggggg cccacaagga cctgggggca 3961 tgagcgcctt cacccgggtg aacctcagtc ctaaccgcaa ccagagtgcc aaagtgatcc 4021 gtgcagaccc acaagggtgc cggcggaggc atagctccga gactttctcc tcaacaccca 4081 gtgccacccg ggtgggcaac acagtgccct ttggagcggg ggcagcagta gggggcggtg 4141 gcggtagcag cagcagcagc gaggatgtga aacgccacag ctctgcttcc tttgagaatg 4201 tgtggctgag gcctggggag cttgggggag cccccaagga gccagccaaa ctgtgtgggg 4261 ctgctggggg tttggagaat ggtcttaact acatagacct ggatttggtc aaggacttca 4321 aacagtgccc tcaggagtgc acccctgaac cgcagcctcc cccaccccca ccccctcatc 4381 aacccctggg cagcggtgag agcagctcca cccgccgctc aagtgaggat ttaagcgcct 4441 atgccagcat cagtttccag aagcagccag aggaccgtca gGGATCCGAC TACAAAGACC 4501 ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAG 4561 GGCCCGATAT CTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 4621 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 4681 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC 4741 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC 4801 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT 4861 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG 4921 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC 4981 CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA 5041 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG 5101 GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 5161 CCCGCCTGGA AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC 5221 CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA 5281 TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG 5341 GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG 5401 CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC TGCGCAGCTG 5461 GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 5521 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT 5581 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT 5641 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG 5701 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 5761 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC 5821 GGTCTATTCT TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA 5881 GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT 5941 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 6001 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 6061 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 6121 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 6181 GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 6241 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 6301 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 6361 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 6421 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 6481 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 6541 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 6601 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 6661 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 6721 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 6781 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 6841 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 6901 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 6961 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 7021 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 7081 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC 7141 CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA 7201 AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA 7261 TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA 7321 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 7381 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA 7441 GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT 7501 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 7561 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 7621 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 7681 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 7741 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 7801 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 7861 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 7921 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 7981 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 8041 CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 8101 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 8161 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 8221 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 8281 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 8341 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 8401 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 8461 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 8521 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 8581 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 8641 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 8701 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 8761 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 8821 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 8881 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 8941 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 9001 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 9061 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 9121 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 9181 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 9241 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 9301 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 9361 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 9421 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 9481 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 9541 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 9601 AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTC GACTCTCAGT 9661 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG 9721 GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT 9781 TGCATGAAGA ATCTGCTTAG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.