pCMV-TMEM106B-3×FLAG-T2A-EGFP-

pCMV-TMEM106B-3×FLAG-T2A-EGFP-Neo plasmid

PVT108001

pCMV-TMEM106B-3×FLAG-T2A-EGFP-Neo plasmid Information

Plasmid name: pCMV-TMEM106B(human)-3×FLAG-T2A-EGFP-Neo
Alias: NM_018374.4;HLD16
Gene lenght: 822
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TMEM106B-3×FLAG-T2A-EGFP-Neo plasmid Description

pCMV-TMEM106B-3×FLAG-T2A-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TMEM106B-3×FLAG-T2A-EGFP-Neo plasmid Sequence

1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat 61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc 841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag 901 cgtttaaact taagcttggt accGCCACCa tgggaaagtc tctttctcat ttgcctttgc 961 attcaagcaa agaagatgct tatgatggag tcacatctga aaacatgagg aatggactgg 1021 ttaatagtga agtccataat gaagatggaa gaaatggaga tgtctctcag tttccatatg 1081 tggaatttac aggaagagat agtgtcacct gccctacttg tcagggaaca ggaagaattc 1141 ctagggggca agaaaaccaa ctggtggcat tgattccata tagtgatcag agattaaggc 1201 caagaagaac aaagctgtat gtgatggctt ctgtgtttgt ctgtctactc ctttctggat 1261 tggctgtgtt tttccttttc cctcgctcta tcgacgtgaa atacattggt gtaaaatcag 1321 cctatgtcag ttatgatgtt cagaagcgta caatttattt aaatatcaca aacacactaa 1381 atataacaaa caataactat tactctgtcg aagttgaaaa catcactgcc caagttcaat 1441 tttcaaaaac agttattgga aaggcacgct taaacaacat aaccattatt ggtccacttg 1501 atatgaaaca aattgattac acagtaccta ccgttatagc agaggaaatg agttatatgt 1561 atgatttctg tactctgata tccatcaaag tgcataacat agtactcatg atgcaagtta 1621 ctgtgacaac aacatacttt ggccactctg aacagatatc ccaggagagg tatcagtatg 1681 tcgactgtgg aagaaacaca acttatcagt tggggcagtc tgaatattta aatgtacttc 1741 agccacaaca ggaattcatg GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA 1801 TCGACTACAA GGATGACGAT GACAAGccgc gggagggcag aggaagtctg ctaacatgcg 1861 gtgacgtcga ggagaatcct ggcccagccc gggatccacc ggtcgccacc atggtgagca 1921 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 1981 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 2041 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 2101 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 2161 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 2221 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 2281 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 2341 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 2401 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 2461 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 2521 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 2581 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgct 2641 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 2701 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 2761 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 2821 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 2881 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 2941 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 3001 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 3061 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 3121 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 3181 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 3241 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 3301 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 3361 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 3421 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 3481 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 3541 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 3601 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 3661 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 3721 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 3781 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 3841 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 3901 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 3961 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 4021 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 4081 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 4141 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 4201 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 4261 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 4321 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 4381 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 4441 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 4501 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 4561 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 4621 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 4681 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 4741 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 4801 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 4861 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 4921 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 4981 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 5041 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 5101 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 5161 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 5221 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 5281 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 5341 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 5401 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 5461 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 5521 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 5581 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 5641 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 5701 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 5761 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 5821 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca 5881 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 5941 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 6001 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 6061 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 6121 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6181 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6241 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6301 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6361 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6421 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6481 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6541 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6601 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6661 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6721 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6781 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6841 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6901 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6961 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 7021 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7081 t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.