pLV3-CMV-Ddr2-3×HA-CopGFP-Puro

pLV3-CMV-Ddr2-3×HA-CopGFP-Puro plasmid

PVT107806

pLV3-CMV-Ddr2-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Ddr2(mouse)-3×HA-CopGFP-Puro
Alias: NM_022563.2;Ntrkr3; tyro10
Gene lenght: 2565
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Ddr2-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-Ddr2-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Ddr2-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga tcccgattcc cagaatgccc 2341 ctggtgctgc tcctgctctt gctcatcctg ggttctgcaa aagctcaggt taatccagcc 2401 atatgccgct atcctctggg catgtcagga ggccacattc cagatgagga catcacagcc 2461 tcaagtcagt ggtcagaatc cacggctgcc aaatatggga ggctggactc tgaagaagga 2521 gatggagcct ggtgtcctga gattccagtg caacccgatg acctgaagga atttctgcag 2581 attgacttgc gaaccctaca ctttatcact cttgtgggga cccaggggcg ccatgcaggg 2641 ggtcatggca ttgaatttgc acccatgtac aagatcaact acagtcggga tggcagtcgc 2701 tggatctcct ggcgtaaccg gcatgggaag caggtgcttg atggaaacag taacccttat 2761 gatgtattcc tgaaggactt ggagccaccc atcgtcgcca gatttgttcg ccttatccca 2821 gtcactgacc actccatgaa cgtgtgcatg agggttgagc tttatggttg tgtctggcta 2881 gatggcttgg tatcctacaa tgctccagct ggacagcagt ttgtactccc tggaggctcc 2941 atcatttatc tgaatgattc tgtctatgat ggagctgttg ggtacagcat gactgaaggg 3001 ctaggccagt tgactgatgg agtatccggc ctggatgatt ttacccagac ccatgaatac 3061 cacgtgtggc ctggctatga ctacgtggga tggcggaacg aaagtgctac caacggtttc 3121 attgagatca tgtttgaatt tgaccgaatc aggaatttta ctaccatgaa ggtccactgc 3181 aacaacatgt ttgctaaagg tgtgaagatt tttaaggagg tccagtgcta ctttcgctcg 3241 gaagccagcg agtgggaacc cactgctgtc tactttcccc tggtcctgga cgatgtgaac 3301 cccagtgccc ggtttgtcac ggtgcccctc caccaccgaa tggccagtgc catcaagtgc 3361 caataccatt ttgccgacac gtggatgatg ttcagcgaga tcactttcca atcagatgct 3421 gcaatgtata acaactctgg agcccttccc acctctccta tggcacccac cacctatgat 3481 cccatgctta aagttgatga tagcaacact cggatcctga ttggttgctt ggtggccatc 3541 atcttcatcc tgctggctat catcgtcatc atcctgtgga ggcagttctg gcagaagatg 3601 ctagaaaagg cttcacggag gatgctggat gatgaaatga cagtcagcct ttccctgccc 3661 agcgagtcca gcatgttcaa taacaaccgc tcctcatcac caagtgaaca ggagtccaac 3721 tctacttatg atcgaatctt cccccttcgc cctgactacc aggagccatc cagactgatc 3781 cgaaagcttc cagagtttgc tccaggagag gaggagtcag ggtgcagtgg tgttgtgaag 3841 ccggcccagc ccaatggacc tgagggcgtg ccccactatg cagaagccga catagtgaat 3901 ctccagggag tgacaggtgg caacacctac tgtgtgcctg ctgtaaccat ggatctgcta 3961 tcggggaaag atgtggctgt ggaagagttc cccaggaaac tgttggcctt caaggagaag 4021 ctgggagaag gccagtttgg ggaggttcat ctctgtgaag tggagggaat ggaaaaattc 4081 aaagacaaag attttgcact agatgtcagt gccaaccagc ctgtcctggt ggccgtgaaa 4141 atgctccgag cagatgccaa caagaatgcc aggaatgatt ttcttaagga gatcaagatc 4201 atgtctcggc tcaaggaccc aaacatcatc cgtctcttag ctgtgtgcat cactgaggac 4261 ccgctctgca tgatcacgga atacatggag aatggagatc ttaatcagtt tctttctcgc 4321 cacgagcctc tgagttcctg ttctagtgat gccacagtca gttacgccaa cctgaagttt 4381 atggcaaccc agattgcctc tggtatgaag tacctttcgt ctctcaactt tgtccaccga 4441 gatctggcca cacgaaactg tttagtgggc aagaattaca ccatcaagat agctgatttt 4501 ggcatgagca gaaacctgta cagtggtgat tactaccgga tccagggccg ggcggtgctc 4561 cccattcgct ggatgtcctg ggaaagcatc ttgctgggca aattcaccac ggcaagtgat 4621 gtgtgggcct ttggggtgac tctgtgggag accttcacct tttgccagga gcagccctat 4681 tcccagctgt cggatgagca ggttatcgag aacactggag agttcttccg agaccaaggg 4741 aggcagatct atctccctca accagccctt tgccccgact ctgtgtataa gctgatgctc 4801 agctgctgga gaagagaaac caagcaccgg ccatccttcc aggaaataca cctcctgctt 4861 cttcagcaag gagccgagCT CGAGTACCCA TACGACGTAC CTGATTATGC AACTTATCCT 4921 TATGATGTAC CTGATTATGC TAGCTATCCT TATGATGTCC CAGACTACGC ATAGggatcc 4981 CTGCGATATC CAGCgcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 5041 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 5101 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 5161 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 5221 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 5281 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 5341 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 5401 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 5461 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 5521 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 5581 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 5641 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 5701 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 5761 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 5821 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 5881 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 5941 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 6001 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 6061 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 6121 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 6181 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 6241 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 6301 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 6361 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 6421 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 6481 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 6541 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 6601 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 6661 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 6721 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 6781 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 6841 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 6901 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 6961 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 7021 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 7081 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 7141 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 7201 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 7261 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 7321 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 7381 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 7441 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 7501 cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc 7561 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 7621 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct 7681 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 7741 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 7801 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 7861 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 7921 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 7981 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 8041 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 8101 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 8161 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact 8221 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 8281 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 8341 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 8401 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 8461 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 8521 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 8581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 8641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 8701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 8761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 8821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 8881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 8941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 9001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 9061 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 9121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 9181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 9241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 9301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 9361 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 9421 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 9481 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 9541 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 9601 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 9661 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 9721 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 9781 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 9841 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 9901 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 9961 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 10021 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 10081 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 10141 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 10201 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 10261 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 10321 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 10381 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 10441 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa 10501 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 10561 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 10621 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 10681 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt 10741 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 10801 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 10861 cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.