pLV3-CMV-LIG4-3×HA-EF1a-mCherr

pLV3-CMV-LIG4-3×HA-EF1a-mCherry-Puro plasmid

PVT107721

pLV3-CMV-LIG4-3×HA-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-LIG4(human)-3×HA-EF1a-mCherry-Puro
Alias: NM_001352604.2;LIG4S
Gene lenght: 2769
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LIG4-3×HA-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-LIG4-3×HA-EF1a-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-LIG4-3×HA-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggcaac cccgcaggaa accatcaaga tctcatttta 2341 cagctgggat tctctggttc acagaggtaa cggagcttgc ccgaggccag atttgtgttc 2401 aactttagaa cgaatacaga aaagtaaagg acgtgcagaa aaaatcagac acttcaggga 2461 atttttagat tcttggagaa aatttcatga tgctcttcat aagaaccaca aagatgtcac 2521 agactctttt tatccagcaa tgagactaat tcttcctcag ctagaaagag agagaatggc 2581 ctatggaatt aaagaaacta tgcttgctaa gctttatatt gagttgctta atttacctag 2641 agatggaaaa gatgccctca aacttttaaa ctacagaaca cccactggaa ctcatggaga 2701 tgctggagac tttgcaatga ttgcatattt tgtgttgaag ccaagatgtt tacagaaagg 2761 aagtttaacc atacagcaag taaacgacct tttagactca attgccagca ataattctgc 2821 taaaagaaaa gacctaataa aaaagagcct tcttcaactt ataactcaga gttcagcact 2881 tgagcaaaag tggcttatac ggatgatcat aaaggattta aagcttggtg ttagtcagca 2941 aactatcttt tctgtttttc ataatgatgc tgctgagttg cataatgtca ctacagatct 3001 ggaaaaagtc tgtaggcaac tgcatgatcc ttctgtagga ctcagtgata tttctatcac 3061 tttattttct gcatttaaac caatgctagc tgctattgca gatattgagc acattgagaa 3121 ggatatgaaa catcagagtt tctacataga aaccaagcta gatggtgaac gtatgcaaat 3181 gcacaaagat ggagatgtat ataaatactt ctctcgaaat ggatataact acactgatca 3241 gtttggtgct tctcctactg aaggttctct taccccattc attcataatg cattcaaagc 3301 agatatacaa atctgtattc ttgatggtga gatgatggcc tataatccta atacacaaac 3361 tttcatgcaa aagggaacta agtttgatat taaaagaatg gtagaggatt ctgatctgca 3421 aacttgttat tgtgtttttg atgtattgat ggttaataat aaaaagctag ggcatgagac 3481 tctgagaaag aggtatgaga ttcttagtag tatttttaca ccaattccag gtagaataga 3541 aatagtgcag aaaacacaag ctcatactaa gaatgaagta attgatgcat tgaatgaagc 3601 aatagataaa agagaagagg gaattatggt aaaacaacct ctatccatct acaagccaga 3661 caaaagaggt gaagggtggt taaaaattaa accagagtat gtcagtggac taatggatga 3721 attggacatt ttaattgttg gaggatattg gggtaaagga tcacggggtg gaatgatgtc 3781 tcattttctg tgtgcagtag cagagaagcc ccctcctggt gagaagccat ctgtgtttca 3841 tactctctct cgtgttgggt ctggctgcac catgaaagaa ctgtatgatc tgggtttgaa 3901 attggccaag tattggaagc cttttcatag aaaagctcca ccaagcagca ttttatgtgg 3961 aacagagaag ccagaagtat acattgaacc ttgtaattct gtcattgttc agattaaagc 4021 agcagagatc gtacccagtg atatgtataa aactggctgc accttgcgtt ttccacgaat 4081 tgaaaagata agagatgaca aggagtggca tgagtgcatg accctggacg acctagaaca 4141 acttaggggg aaggcatctg gtaagctcgc atctaaacac ctttatatag gtggtgatga 4201 tgaaccacaa gaaaaaaagc ggaaagctgc cccaaagatg aagaaagtta ttggaattat 4261 tgagcactta aaagcaccta accttactaa cgttaacaaa atttctaata tatttgaaga 4321 tgtagagttt tgtgttatga gtggaacaga tagccagcca aagcctgacc tggagaacag 4381 aattgcagaa tttggtggtt atatagtaca aaatccaggc ccagacacgt actgtgtaat 4441 tgcagggtct gagaacatca gagtgaaaaa cataattttg tcaaataaac atgatgttgt 4501 caagcctgca tggcttttag aatgttttaa gaccaaaagc tttgtaccat ggcagcctcg 4561 ctttatgatt catatgtgcc catcaaccaa agaacatttt gcccgtgaat atgattgcta 4621 tggtgatagt tatttcattg atacagactt gaaccaactg aaggaagtat tctcaggaat 4681 taaaaattct aacgagcaga ctcctgaaga aatggcttct ctgattgctg atttagaata 4741 tcggtattcc tgggattgct ctcctctcag tatgtttcga cgccacaccg tttatttgga 4801 ctcgtatgct gttattaatg acctgagtac caaaaatgag gggacaaggt tagctattaa 4861 agccttggag cttcggtttc atggagcaaa agtagtttct tgtttagctg agggagtgtc 4921 tcatgtaata attggggaag atcatagtcg tgttgcagat tttaaagctt ttagaagaac 4981 ttttaagaga aagtttaaaa tcctaaaaga aagttgggta actgattcaa tagacaagtg 5041 tgaattacaa gaagaaaacc agtatttgat tggatccTAC CCATACGACG TACCTGATTA 5101 TGCAACTTAT CCTTATGATG TACCTGATTA TGCTAGCTAT CCTTATGATG TCCCAGACTA 5161 CGCATAGgcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca 5221 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga 5281 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 5341 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 5401 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc 5461 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct 5521 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg 5581 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc 5641 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat 5701 ccaagctgtg accggcgcct acgctagacg ccaccatggt gagcaagggc gaggaggata 5761 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg 5821 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg 5881 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc 5941 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga 6001 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg 6061 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc 6121 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg 6181 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga 6241 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca 6301 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc 6361 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg 6421 gcggcatgga cgagctgtac aagggatctc gcgagggcag aggaagtctt ctaacatgcg 6481 gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca 6541 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 6601 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 6661 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 6721 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 6781 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 6841 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 6901 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 6961 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 7021 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 7081 gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat 7141 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 7201 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 7261 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 7321 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 7381 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 7441 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 7501 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 7561 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 7621 cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atctcccttt 7681 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta 7741 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag 7801 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 7861 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 7921 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 7981 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 8041 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 8101 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 8161 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 8221 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 8281 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 8341 tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc 8401 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 8461 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 8521 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 8581 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 8641 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 8701 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 8761 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 8821 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 8881 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 8941 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 9001 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 9061 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 9121 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 9181 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 9241 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 9301 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 9361 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 9421 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 9481 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 9541 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 9601 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 9661 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 9721 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 9781 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 9841 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 9901 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 9961 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 10021 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 10081 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 10141 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 10201 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 10261 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 10321 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 10381 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 10441 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 10501 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 10561 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 10621 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 10681 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 10741 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 10801 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 10861 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 10921 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 10981 acgttgtaaa acgacggcca gtgccaagct g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.