pLV3-CMV-Ugcg-3×FLAG-CopGFP-Pu

pLV3-CMV-Ugcg-3×FLAG-CopGFP-Puro plasmid

PVT107673

pLV3-CMV-Ugcg-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Ugcg(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_011673.3;Epcs21; GlcT-1; Ugcgl
Gene lenght: 1182
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Ugcg-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Ugcg-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Ugcg-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cgctgctgga cctggcccag 2341 gagggaatgg ccttgttcgg cttcgtgctc ttcgtggtgc tgtggctgat gcatttcatg 2401 tccatcatct acacccggtt acacctcaac aagaaggcaa cagacaaaca gccgtatagc 2461 aagctccctg gtgtctctct tctgaagcca ctgaaggggg tggatcctaa cctaatcaac 2521 aacttggaga cattctttga actggattat cccaaatatg aagtactcct ttgtgtacaa 2581 gatcatgatg atccagccat tgatgtatgt aagaaattgc ttggaaaata cccaaatgtc 2641 gatgctagat tatttatagg tggcaaaaag gttggcatta accctaaaat taataatttg 2701 atgccagcat atgaagttgc aaaatatgat ctcatatgga tttgtgatag tggaataaga 2761 gtcatcccag acacattaac tgacatggtg aatcagatga cagagaaagt ggggttggtc 2821 cacgggctgc cgtatgtagc cgacagacaa ggctttgctg ccaccttaga gcaggtatat 2881 tttggaactt cacacccaag atcctatatc tctgccaatg taactggctt caaatgtgtg 2941 acggggatgt cttgtttgat gaggaaggat gtgctagatc aggcaggagg gctcatagcc 3001 tttgctcagt acattgctga agattacttt atggccaaag caatagccga ccgaggttgg 3061 aggttttcaa tgtctactca agttgccatg caaaactctg gttcgtactc aatttctcag 3121 tttcaatcca gaatgatcag gtggaccaaa ttgagaatta acatgcttcc tgctacaata 3181 atttgtgagc caatttcaga atgctttgtt gccagtttaa ttattgggtg ggcagcccac 3241 catgtattca gatgggatat catggtcttc ttcatgtgcc actgcctggc atggtttata 3301 tttgactaca ttcaactcag gggtgtccag ggtggcacac tgtgtttttc aaaacttgat 3361 tatgctgtgg cctggttcat ccgtgaatcc atgacaatct acattttcct gtcggcatta 3421 tgggacccga ctataagctg gagaactggt cgctacaggt tacgctgtgg ggggacagca 3481 gaggagatcc tggatgtggg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 3541 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 3601 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3661 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 3721 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 3781 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3841 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 3901 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 3961 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4021 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4081 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 4141 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 4201 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 4261 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 4321 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 4381 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 4441 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4501 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4561 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4621 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4681 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 4741 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 4801 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 4861 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 4921 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 4981 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5041 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5101 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5161 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5221 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5281 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5341 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5401 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5461 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5521 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 5581 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5641 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5701 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 5761 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 5821 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5881 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 5941 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 6001 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6061 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 6121 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 6181 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 6241 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6301 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6361 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6421 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6481 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6541 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6601 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 6661 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 6721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 6781 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 6841 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6901 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6961 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 7021 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7081 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 7141 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 7201 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 7261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 7441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7681 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7921 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7981 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8041 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8101 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 8161 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 8221 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 8281 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 8341 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8401 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8461 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8521 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8581 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8641 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8701 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 8761 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8821 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8881 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8941 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9001 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 9061 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 9121 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 9181 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 9241 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 9301 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 9361 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 9421 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.