pLV3-CMV-PTPRM-3×FLAG-Puro pla

pLV3-CMV-PTPRM-3×FLAG-Puro plasmid

PVT107630

pLV3-CMV-PTPRM-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-PTPRM(human)-3×FLAG-Puro
Alias: NM_001105244.2;hR-PTPu; PTPRL1; R-PTP-MU; RPTPM; RPTPU
Gene lenght: 4395
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PTPRM-3×FLAG-Puro plasmid Description

pLV3-CMV-PTPRM-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PTPRM-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgagggga cttgggactt gcctggcgac tttggccgga 2341 cttttgctaa ctgcggcggg cgagacgttc tcaggtggct gcctctttga tgagccgtat 2401 agcacatgtg gatatagtca atctgaaggt gatgacttca attgggagca agtgaacacc 2461 ttgactaaac cgacttctga tccatggatg ccatcaggtt ctttcatgct ggtgaatgcc 2521 tctgggagac ctgaggggca gagagcccac ctgctcttac cccaacttaa agaaaatgac 2581 acccactgca tcgattttca ctattttgtg tccagcaaga gtaattctcc tccggggtta 2641 ctcaatgtct acgtgaaggt caataacggg ccactgggga atcctatctg gaatatatct 2701 ggagacccaa cacgtacatg gaacagggca gaactggcca ttagtacttt ctggcctaac 2761 ttttatcagg tgatttttga agtgataact tctggacatc aaggctatct cgctatcgat 2821 gaggtgaagg tgttaggaca tccatgtacc aggactcctc acttcctgcg gattcagaat 2881 gtggaagtta atgctggcca gtttgctacc ttccagtgca gtgccatcgg caggaccgtg 2941 gcaggagaca ggctctggtt acagggcatt gatgtgcgag atgctcctct gaaggaaatc 3001 aaggtgacca gctcccgacg cttcattgct tcatttaatg ttgtgaatac caccaaacga 3061 gatgctggaa agtaccgctg catgattcgc actgaaggag gtgttggaat atcaaactat 3121 gcagagttgg tagttaaaga accacccgtt cctattgccc cacctcagct cgcctctgta 3181 ggagccacct acctgtggat acagctcaac gccaactcca tcaatgggga tgggcccatt 3241 gtggcccgag aggtggagta ctgcacggcc agtgggagct ggaatgaccg gcagccagtc 3301 gattccacga gctataaaat tggacacctt gacccagata cagaatatga gattagtgtg 3361 ctcctgacca ggccagggga gggtggcact ggctctcctg gtccagctct caggacaaga 3421 acaaagtgtg ctgatcccat gcgaggccca agaaaactag aagtagtgga ggtcaaatct 3481 cggcaaatca ctatccgctg ggagccattt ggatataatg taactcgttg ccacagttat 3541 aatctcactg tccactactg ttaccaagtt ggaggacaag aacaagtgcg agaagaagta 3601 agctgggata cagaaaactc acaccctcaa cacacgatca ctaacctgtc accatacacc 3661 aatgtcagtg tgaaactgat cctcatgaac ccagagggcc ggaaggaaag ccaagaactc 3721 atagtgcaga cagatgaaga cctcccaggt gctgttccca ctgaatccat acaaggaagt 3781 acctttgaag agaagatatt tcttcagtgg agagaaccaa ctcaaacata tggtgtaatc 3841 actttatatg agatcaccta caaagcagtc agttcctttg acccagaaat agatttatcc 3901 aatcagagtg gaagagtttc aaagctggga aatgaaaccc attttctgtt ttttggactg 3961 tatccgggga ccacatactc ctttaccatc cgagctagca cagctaaggg ttttgggcct 4021 ccagcaacaa accagttcac caccaaaata tcagcaccct ctatgccagc ttatgaactt 4081 gagacacctt tgaatcaaac tgacaatacc gtgacagtca tgctgaaacc tgcccacagc 4141 agaggagcac ctgtcagtgt ctatcaaata gttgttgagg aagaacgtcc tcgaagaact 4201 aaaaagacga cagaaatctt aaagtgctac ccagtgccaa ttcacttcca gaatgcttct 4261 ctgctgaact cacagtacta ctttgctgca gaatttcctg cagacagcct ccaagctgcg 4321 cagcctttta caattggtga taataagaca tataatggat actggaacac tccccttctc 4381 ccctataaaa gctacagaat ttatttccaa gctgctagta gagccaatgg ggaaaccaaa 4441 atagactgtg tccaagtggc cacaaaagga gctgccactc cgaaaccagt cccagaaccc 4501 gagaaacaga cagaccatac agttaaaatt gctggagtca tcgcgggcat cttgctgttc 4561 gtgattatat ttcttggagt tgtgttggta atgaagaaaa ggaaactggc caagaagcgg 4621 aaagagacca tgagcagcac ccgacaggag atgactgtga tggtgaactc aatggacaag 4681 agctatgctg agcagggcac aaactgcgac gaggctttct cattcatgga cacgcacaat 4741 ctgaatggga gatctgtgtc ttcaccatcg tccttcacaa tgaaaacaaa tacactgagc 4801 acatcggtgc ctaattccta ttacccagac ccatttgtgc caactgcaat tttagtgcca 4861 ataaatgatg aaacccacac aatggccagc gataccagca gcctggtgca gtcccatact 4921 tacaagaagc gagagccggc cgacgtgccc tatcagactg ggcagctcca ccccgccatc 4981 cgggtggcag acctccttca gcacatcaca cagatgaagt gtgcggaggg ctacggcttc 5041 aaggaggaat acgagagctt ctttgaaggg cagtctgcac catgggactc ggctaagaaa 5101 gatgagaaca gaatgaagaa cagatacggg aatatcattg catacgatca ttcccgagtg 5161 aggctgcaga caatagaagg agacacaaac tcagactata tcaatggcaa ttatatcgat 5221 ggttatcatc gacccaatca ttacattgct acccaagggc caatgcagga aaccatctat 5281 gacttctgga ggatggtgtg gcacgaaaac actgcaagta tcatcatggt gaccaatctt 5341 gtggaagtgg gaagggtcaa atgctgcaaa tactggccag atgacacaga gatatataaa 5401 gacattaaag ttaccctaat agaaacagaa ctactggcag aatatgtgat aagaacattt 5461 gctgttgaaa agagaggtgt gcatgaaatc cgagagatca gacagtttca cttcactggc 5521 tggccggatc atggggtccc ctaccatgcc accggcctgc tgggattcgt gcggcaagtc 5581 aagtccaaga gcccgcccag tgcaggccca ctggtggtgc actgcagtgc tggtgcaggg 5641 aggactggct gtttcatcgt cattgatatc atgttggaca tggccgaaag ggaaggggtc 5701 gtagacatct acaactgcgt cagggagctg cggtcacgga gggtgaacat ggtgcaaaca 5761 gaggagcagt atgtgtttat ccacgatgcg atcctggaag cctgtctttg tggggacacc 5821 tctgtgcctg cttcccaagt taggtctctg tattatgaca tgaacaaact ggatccacag 5881 acaaactcaa gccagattaa agaggaattc cggacgctaa acatggtgac accaacgctg 5941 cgagtagagg actgcagcat cgcactgttg ccccggaacc atgagaaaaa ccggtgcatg 6001 gacatcctgc ccccagaccg ctgcctgccc ttcctcatca ccatcgatgg ggagagcagc 6061 aactacatca atgctgccct catggacagc tataaacagc cttcagcttt tatagtcacc 6121 cagcatcctt tgccaaacac agtgaaagac ttttggagac tggtcctgga ttatcactgc 6181 acatccgtag ttatgctaaa tgatgtggat cctgcccagt tgtgtccaca gtactggcca 6241 gaaaacggag tacacagaca cggccccatc caggtggaat ttgtctctgc tgacctggaa 6301 gaggacatca tcagcaggat attccgcatt tacaatgccg ccagacccca agatggatat 6361 cggatggtgc agcaattcca gttcctgggc tggccgatgt acagggacac accagtgtct 6421 aagcgctcct tcttgaagct cattcgccag gtggacaagt ggcaagagga gtacaatggc 6481 ggggaaggcc gcacggttgt gcactgcttg aacgggggag gccgcagtgg gacgttctgc 6541 gccatcagca tcgtatgtga gatgctccgg caccagagaa ccgtggatgt ctttcacgct 6601 gtgaagacac tgaggaacaa caagcccaac atggtcgacc tcctggatca gtacaagttc 6661 tgctacgagg tggccctgga atacttgaat tctggcGGAT CCGACTACAA AGACCATGAC 6721 GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GCGGCCGCGA 6781 AGGATCTGCG ATCGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC 6841 GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA 6901 AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG 6961 TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA 7021 CAGCTGAAGC TTCGAGGGGC TCGCATCTCT CCTTCACGCG CCCGCCGCCC TACCTGAGGC 7081 CGCCATCCAC GCCGGTTGAG TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC CTCCTGAACT 7141 GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC 7201 CCTTGGAGCC TACCTAGACT CAGCCGGCTC TCCACGCTTT GCCTGACCCT GCTTGCTCAA 7261 CTCTACGTCT TTGTTTCGTT TTCTGTTCTG CGCCGTTACA GATCCAAGCT GTGACCGGCG 7321 CCTACGCTAG ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 7381 CCGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 7441 CGACCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 7501 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 7561 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA 7621 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 7681 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA 7741 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC 7801 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 7861 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 7921 CGGTGCCTGA GTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 7981 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 8041 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC 8101 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC 8161 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT 8221 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG 8281 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC 8341 CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA 8401 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG 8461 GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 8521 CCCGCCTGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT 8581 TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAAATAA GATCTGCTTT 8641 TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC 8701 TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG 8761 CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA 8821 AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA 8881 AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 8941 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 9001 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA GCTATCCCGC CCCTAACTCC 9061 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC 9121 CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT 9181 AGACTTTTGC AGAGACGGCC CAAATTCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA 9241 TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 9301 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA 9361 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG 9421 TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG 9481 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 9541 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 9601 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 9661 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 9721 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 9781 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 9841 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 9901 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 9961 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 10021 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 10081 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 10141 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 10201 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 10261 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 10321 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 10381 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 10441 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 10501 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 10561 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 10621 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 10681 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 10741 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 10801 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 10861 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 10921 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 10981 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 11041 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 11101 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 11161 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 11221 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 11281 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 11341 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT 11401 AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG 11461 TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC 11521 CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT 11581 TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC 11641 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA 11701 CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG 11761 ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA 11821 AACGACGGCC AGTGCCAAGC TG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.