pCMV-RELB-EGFP-Neo plasmid

pCMV-RELB-EGFP-Neo plasmid

PVT107627

pCMV-RELB-EGFP-Neo plasmid Information

Plasmid name: pCMV-RELB(human)-EGFP-Neo
Alias: NM_001411087.1;I-REL; IMD53; IREL; REL-B
Gene lenght: 1728
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-RELB-EGFP-Neo plasmid Description

pCMV-RELB-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-RELB-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgaggc caccatgctt cggtctgggc cagcctctgg gccgtccgtc 661 cccactggcc gggccatgcc gagtcgccgc gtcgccagac cgccggctgc gccggagctg 721 ggggccttag ggtcccccga cctctcctca ctctcgctcg ccgtttccag gagcacagag 781 atcatcgacg agtacatcaa ggagaacggc ttcggcctgg acgggggaca gccgggcccg 841 ggcgaggggc tgccacgcct ggtgtctcgc ggggctgcgt ccctgagcac ggtcaccctg 901 ggccctgtgg cgcccccagc cacgccgccg ccttggggct gccccctggg ccgactagtg 961 tccccagcgc cgggcccggg cccgcagccg cacctggtca tcacggagca gcccaagcag 1021 cgcggcatgc gcttccgcta cgagtgcgag ggccgctcgg ccggcagcat ccttggggag 1081 agcagcaccg aggccagcaa gacgctgccc gccatcgagc tccgggattg tggagggctg 1141 cgggaggtgg aggtgactgc ctgcctggtg tggaaggact ggcctcaccg agtccacccc 1201 cacagcctcg tggggaaaga ctgcaccgac ggcatctgca gggtgcggct ccggcctcac 1261 gtcagccccc ggcacagttt taacaacctg ggcatccagt gtgtgaggaa gaaggagatt 1321 gaggctgcca ttgagcggaa gattcaactg ggcattgacc cctacaacgc tgggtccctg 1381 aagaaccatc aggaagtaga catgaatgtg gtgaggatct gcttccaggc ctcatatcgg 1441 gaccagcagg gacagatgcg ccggatggat cctgtgcttt ccgagcccgt ctatgacaag 1501 aaatccacaa acacatcaga gctgcggatt tgccgaatta acaaggaaag cgggccgtgc 1561 accggtggcg aggagctcta cttgctctgc gacaaggtgc agaaagagga catatcagtg 1621 gtgttcagca gggcctcctg ggaaggtcgg gctgacttct cccaggccga cgtgcaccgc 1681 cagattgcca ttgtgttcaa gacgccgccc tacgaggacc tggagattgt cgagcccgtg 1741 acagtcaacg tcttcctgca gcggctcacc gatggggtct gcagcgagcc attgcctttc 1801 acgtacctgc ctcgcgacca tgacagctac ggcgtggaca agaagcggaa acgggggatg 1861 cccgacgtcc ttggggagct gaacagctct gacccccatg gcatcgagag caaacggcgg 1921 aagaaaaagc cggccatcct ggaccacttc ctgcccaacc acggctcagg cccgttcctc 1981 ccgccgtcag ccctgctgcc agaccctgac ttcttctctg gcaccgtgtc cctgcccggc 2041 ctggagcccc ctggcgggcc tgacctcctg gacgatggct ttgcctacga ccctacggcc 2101 cccacactct tcaccatgct ggacctgctg cccccggcac cgccacacgc tagcgctgtt 2161 gtgtgcagcg gaggtgccgg ggccgtggtt ggggagaccc ccggccctga accactgaca 2221 ctggactcgt accaggcccc gggccccggg gatggaggca ccgccagcct tgtgggcagc 2281 aacatgttcc ccaatcatta ccgcgaggcg gcctttgggg gcggcctcct atccccgggg 2341 cctgaagcca cgggtggagg cggatcagga ggcggtggat ctatggtgag caagggcgag 2401 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 2461 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 2521 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 2581 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag 2641 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 2701 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 2761 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 2821 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc 2881 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 2941 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc 3001 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 3061 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga 3121 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc 3181 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag 3241 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 3301 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta 3361 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 3421 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 3481 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 3541 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 3601 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 3661 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 3721 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 3781 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga 3841 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 3901 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc 3961 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 4021 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 4081 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 4141 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 4201 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 4261 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga 4321 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 4381 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 4441 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 4501 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 4561 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 4621 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 4681 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 4741 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 4801 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 4861 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 4921 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 4981 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 5041 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 5101 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 5161 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 5221 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 5281 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg 5341 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt 5401 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac 5461 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg 5521 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt 5581 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 5641 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 5701 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 5761 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 5821 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 5881 tTcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 5941 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 6001 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 6061 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 6121 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 6181 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 6241 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 6301 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 6361 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 6421 gtattaccgc catgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.