pLV3-CMV-HCRTR2-mCherry-Puro p

pLV3-CMV-HCRTR2-mCherry-Puro plasmid

PVT107622

pLV3-CMV-HCRTR2-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-HCRTR2(human)-mCherry-Puro
Alias: NM_001384272.1;ORXR1; OX1R; OXR1
Gene lenght: 1332
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HCRTR2-mCherry-Puro plasmid Description

pLV3-CMV-HCRTR2-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HCRTR2-mCherry-Puro plasmid Sequence

1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 361 gcgctaccgg ACTCAGATCT CGAGCTCAAG CTTCGAATTC TGCAGTCGAC gccaccatgt 421 ccggcaccaa attggaggac tccccccctt gtcgcaactg gtcatctgct tcggagctga 481 atgaaactca agagcccttt ttaaacccca ccgactatga cgacgaggaa ttcctgcggt 541 acctgtggag ggaatacctg cacccgaaag aatatgagtg ggtcctgatc gccgggtaca 601 tcatcgtgtt cgtcgtggct ctcattggga acgtcctggt ttgtgtggca gtgtggaaga 661 accaccacat gaggacggta accaactact tcatagtcaa tctttctctg gctgatgtgc 721 tcgtgaccat cacctgcctt ccagccacac tggtcgtgga tatcactgag acctggtttt 781 ttggacagtc cctttgcaaa gtgattcctt atctacagac cgtgtcggtg tctgtgtctg 841 tcctcacact gagctgtatc gccttggatc ggtggtatgc aatctgtcac cctttgatgt 901 ttaagagcac agcaaagcgg gcccgtaaca gcattgtcat catctggatt gtctcctgca 961 ttataatgat tcctcaggcc atcgtcatgg agtgcagcac cgtgttccca ggcttagcca 1021 ataaaaccac cctctttacg gtgtgtgatg agcgctgggg tggtgaaatt tatcccaaga 1081 tgtaccacat ctgtttcttt ctggtgacat acatggcacc actgtgtctc atggtgttgg 1141 cttatctgca aatatttcgc aaactctggt gtcgacagat ccctggaaca tcatctgtag 1201 ttcagagaaa atggaagccc ctgcagcctg tttcacagcc tcgagggcca ggacagccaa 1261 cgaagtcccg gatgagcgct gtggcggctg aaataaagca gatccgagcc agaaggaaaa 1321 cagcccggat gttgatgatt gtgcttttgg tatttgcaat ttgctatcta ccaattagca 1381 tcctcaatgt gctaaagaga gtatttggga tgtttgccca tactgaagac agagagactg 1441 tgtatgcctg gtttaccttt tcacactggc ttgtatatgc caatagtgct gcgaatccaa 1501 ttatttataa ttttctcagt ggaaaatttc gagaggaatt taaagctgcg ttttcttgct 1561 gttgccttgg agttcaccat cgccaggagg atcggctcac caggggacga actagcacag 1621 agagccggaa gtccttgacc actcaaatca gcaactttga taacatatca aaactttctg 1681 agcaagttgt gctcactagc ataagcacac tcccagcagc caatggagca ggaccacttc 1741 aaaactggct cgagggcggt ggaggcagtg gtggaggcgg tagcgcACCG GTCGCCACCa 1801 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg 1861 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc 1921 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct 1981 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc 2041 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg 2101 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg 2161 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa 2221 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg 2281 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg 2341 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca 2401 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac 2461 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtGA Attctcgacc 2521 tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac 2581 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa 2641 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt 2701 tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca 2761 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg 2821 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc 2881 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg 2941 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca 3001 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg 3061 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt 3121 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc 3181 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga 3241 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag 3301 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga 3361 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg 3421 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac 3481 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt 3541 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc 3601 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg 3661 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc 3721 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt 3781 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg 3841 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc 3901 atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 3961 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc 4021 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 4081 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 4141 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 4201 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 4261 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 4321 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 4381 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 4441 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 4501 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 4561 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc 4621 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 4681 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 4741 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg 4801 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 4861 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 4921 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 4981 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 5041 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 5101 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 5161 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 5221 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 5281 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 5341 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 5401 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 5461 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 5521 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 5581 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 5641 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 5701 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 5761 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 5821 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 5881 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 5941 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 6001 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 6061 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 6121 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 6181 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 6241 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 6301 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 6361 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 6421 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 6481 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 6541 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 6601 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 6661 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 6721 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 6781 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 6841 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 6901 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 6961 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 7021 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 7081 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 7141 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 7201 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 7261 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 7321 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 7381 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct 7441 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg 7501 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa 7561 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga 7621 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata 7681 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 7741 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 7801 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 7861 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 7921 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 7981 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 8041 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 8101 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 8161 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 8221 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 8281 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 8341 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga 8401 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 8461 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 8521 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 8581 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 8641 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 8701 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 8761 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 8821 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 8881 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 8941 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 9001 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 9061 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 9121 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 9181 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct 9241 cgacggtatc gatcacgaga ctagcctcga gcggccgccc ccttcaccga gggcctattt 9301 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt 9361 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt 9421 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.