pAAV-beta-arrestin2-HA-TEV219

pAAV-beta-arrestin2-HA-TEV219 plasmid

PVT107588

pAAV-beta-arrestin2-HA-TEV219 plasmid Information

Plasmid name: pAAV-beta-arrestin2-HA-TEV219
Alias: (Plasmid #104845)
Gene lenght: 1974
Host: Mammalian cells,Adeno associated virus
Use(s): Protein Expression
Fragment type: ORF
Species: synthesis
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pAAV-beta-arrestin2-HA-TEV219 plasmid Description

pAAV-beta-arrestin2-HA-TEV219 It is a plasmid suitable for protein expression in mammalian cells and also suitable for adeno-associated virus packaging.

pAAV-beta-arrestin2-HA-TEV219 plasmid Sequence

1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT GCGGCCGCAC GCGTTGTACA TAGTAATCAA TTACGGGGTC ATTAGTTCAT 181 AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG 241 CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA 301 GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA 361 CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC 421 GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC 481 GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA 541 TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG 601 TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG 661 CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT 721 AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA 781 GCTTGCCACC ATGGGTGAAA AACCCGGGAC CAGGGTCTTC AAGAAGTCGA GCCCTAACTG 841 CAAGCTCACC GTGTACTTGG GCAAGCGTGA CTTTGTGGAT CACTTGGACA AAGTGGATCC 901 TGTCGATGGT GTGGTGCTTG TGGATCCTGA CTACTTGAAG GACCGGAAAG TGTTTGTGAC 961 CCTCACCTGT GCCTTCCGCT ATGGCCGAGA AGACCTGGAT GTACTGGGCC TGTCTTTCCG 1021 CAAAGATCTG TTCATCGCCA CCTACCAGGC CTTCCCCCCC ATGCCCAACC CACCTCGGCC 1081 CCCCACCCGC CTACAGGACC GACTGCTGAA GAAGTTGGGC CAGCACGCCC ACCCCTTTTT 1141 TTTCACAATA CCCCAGAATT TGCCTTGCTC CGTCACACTG CAGCCAGGAC CGGAGGACAC 1201 AGGGAAGGCC TGTGGAGTAG ACTTTGAGAT TCGAGCCTTC TGTGCCAAAT CTATAGAAGA 1261 AAAAAGCCAC AAAAGGAACT CCGTGCGGCT TATCATCAGA AAGGTACAGT TTGCTCCTGA 1321 GACACCCGGC CCCCAGCCAT CAGCTGAAAC CACACGCCAC TTCCTCATGT CTGACCGGAG 1381 GTCCCTGCAC CTAGAGGCTT CCCTGGACAA AGAGCTGTAC TACCATGGGG AACCCCTCAA 1441 TGTCAACGTC CACGTCACCA ACAATTCTGC CAAAACCGTC AAGAAGATCA GAGTGTCTGT 1501 GAGACAGTAT GCCGACATTT GCCTCTTCAG CACCGCGCAG TACAAGTGTC CTGTGGCTCA 1561 GCTTGAACAA GATGACCAGG TGTCTCCCAG TTCCACATTC TGCAAGGTGT ACACCATAAC 1621 CCCGCTGCTC AGTGACAACC GAGAGAAGCG TGGCCTTGCC CTTGATGGGC AACTCAAGCA 1681 CGAAGACACC AACCTGGCTT CCAGCACCAT TGTGAAGGAG GGAGCCAACA AGGAGGTGCT 1741 GGGAATCCTA GTATCCTACA GGGTCAAGGT GAAGCTGGTG GTGTCTCGAG GCGGGGATGT 1801 CTCCGTGGAG CTACCTTTCG TCCTAATGCA CCCCAAGCCC CACGACCACA TCACCCTTCC 1861 CCGACCCCAG TCAGCCCCCC GGGAAATAGA CATCCCTGTG GATACCAACC TCATTGAATT 1921 CGATACCAAC TATGCCACAG ACGACGACAT CGTGTTTGAG GACTTTGCGA GGCTTCGGCT 1981 GAAGGGGATG AAGGATGACG ACTGTGATGA CCAGTTCTGC TACCCATACG ATGTGCCAGA 2041 TTACGCCTAT CCCTATGACG TCCCTGATTA CGCTGGTGGA AGTGGATCAG GCAGCGGTGG 2101 ATCTGGAGAA AGCTTGTTTA AGGGACCACG TGATTACAAC CCGATATCGA GCACCATTTG 2161 TCATTTGACG AATGAATCTG ATGGGCACAC AACATCGTTG TATGGTATTG GATTTGGTCC 2221 CTTCATCATT ACAAACAAGC ACTTGTTTAG AAGAAATAAT GGAACACTGT TGGTCCAATC 2281 ACTACATGGT GTATTCAAGG TCAAGAACAC CACGACTTTG CAACAACACC TCATTGATGG 2341 GAGGGACATG ATAATTATTC GCATGCCTAA GGATTTCCCA CCATTTCCTC AAAAGCTGAA 2401 ATTTAGAGAG CCACAAAGGG AAGAGCGCAT ATGTCTTGTG ACAACCAACT TCCAAACTAA 2461 GAGCATGTCT AGCATGGTGT CAGACACTAG TTGCACATTC CCTTCATCTG ATGGCATATT 2521 CTGGAAGCAT TGGATTCAAA CCAAGGATGG GCAGTGTGGC AGTCCATTAG TATCAACTAG 2581 AGATGGGTTC ATTGTTGGTA TACACTCAGC ATCGAATTTC ACCAACACAA ACAATTATTT 2641 CACAAGCGTG CCGAAAAACT TCATGGAATT GTTGACAAAT CAGGAGGCGC AGCAGTGGGT 2701 TAGTGGTTGG CGATTAAATG CTGACTCAGT ATTGTGGGGT GGCCATAAAG TTTTCATGGT 2761 GTAAGAATTC CTCGAGCTAG AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG 2821 CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT 2881 GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT 2941 CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT 3001 GCTGGGGAGG TAACCACGTG CGGACCGAGC GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG 3061 CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC 3121 GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCTGC CTGCAGGGGC 3181 GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGTCAAA 3241 GCAACCATAG TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG 3301 CAGCGTGACC GCTACACTTG CCAGCGCCTT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC 3361 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG 3421 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC 3481 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT 3541 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACTCTATCT CGGGCTATTC 3601 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGTCTATTGG TTAAAAAATG AGCTGATTTA 3661 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTTAT GGTGCACTCT 3721 CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC 3781 TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT 3841 CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA 3901 GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC 3961 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT 4021 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG 4081 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC 4141 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA 4201 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA 4261 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG 4321 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC 4381 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC 4441 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT 4501 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA 4561 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG 4621 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT 4681 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG 4741 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG 4801 TGAGCGTGGA AGCCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT 4861 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC 4921 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT 4981 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT 5041 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC 5101 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 5161 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 5221 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 5281 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 5341 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 5401 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 5461 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 5521 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 5581 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 5641 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 5701 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 5761 TTTTGCTCAC ATGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.