pLV3-CMV-mCherry-ULK1-Puro pla

pLV3-CMV-mCherry-ULK1-Puro plasmid

PVT107605

pLV3-CMV-mCherry-ULK1-Puro plasmid Information

Plasmid name: pLV3-CMV-mCherry-ULK1(human-Toxic-opt)-Puro
Alias: NM_003565.4;ATG1; ATG1A; hATG1; UNC51; Unc51.1
Gene lenght: 3153
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: MCHERRY-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-mCherry-ULK1-Puro plasmid Description

pLV3-CMV-mCherry-ULK1-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-mCherry-ULK1-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggtgagc aagggcgagg aggataacat ggccatcatc 2341 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag 2401 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 2461 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc 2521 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 2581 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 2641 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 2701 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag 2761 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag 2821 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag 2881 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac 2941 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag 3001 ctgtacaagg GaGGTGGAGG TAGTGGAGGA GGCGGAtcCA TGGAACCCGG AAGAGGAGGC 3061 ACAGAAACCG TTGGGAAATT CGAATTCTCC AGAAAAGATC TTATTGGGCA TGGGGCTTTT 3121 GCTGTAGTTT TCAAGGGACG GCATCGAGAG AAACATGACC TTGAAGTGGC TGTGAAGTGC 3181 ATCAACAAGA AGAACCTCGC TAAGTCCCAG ACCCTCCTTG GGAAGGAAAT CAAGATCCTG 3241 AAGGAGCTGA AACATGAGAA TATCGTGGCC TTGTATGACT TCCAAGAAAT GGCCAATAGC 3301 GTGTACCTCG TTATGGAATA CTGCAACGGG GGTGATTTGG CCGATTACTT GCATGCTATG 3361 AGGACATTGT CTGAAGACAC TATTCGGCTG TTTCTGCAAC AGATTGCCGG TGCAATGCGG 3421 CTCCTGCACA GCAAGGGCAT AATCCATCGA GATCTCAAGC CGCAGAACAT CCTGCTGTCC 3481 AACCCCGCCG GTAGACGCGC TAATCCAAAT AGCATTAGAG TTAAGATTGC AGATTTTGGC 3541 TTCGCACGGT ACCTGCAGTC AAACATGATG GCCGCCACAT TGTGTGGTAG CCCTATGTAT 3601 ATGGCCCCTG AGGTGATTAT GAGCCAGCAC TACGATGGGA AGGCCGATCT TTGGTCCATA 3661 GGGACTATAG TTTACCAATG TCTTACGGGG AAAGCGCCCT TTCAAGCTAG TTCACCCCAG 3721 GACTTGAGGC TGTTCTACGA GAAAAACAAA ACACTCGTGC CTACCATCCC CAGAGAGACA 3781 TCAGCTCCCC TGCGCCAGTT GCTCCTGGCT TTGCTCCAAA GAAACCACAA AGATAGAatg 3841 gacttcgatg agttttttca tcaccctttc ctcgatgcca gcccctcggt caggaaatcc 3901 ccacccgtgc ctgtgccctc gtacccaagc tcggggtccg gcagcagctc cagcagcagc 3961 tccacctccc acctggcctc cccgccgtcc ctgggcgaga tgcagcagct gcagaagacc 4021 ctggcctccc cggctgacac cgctggcttc ctgcacagct cccgggactc tggtggcagc 4081 aaggactctt cctgtgacac agacgacttc gtcatggtcc ccgcgcagtt tccaggtgac 4141 ctggtggctg aggcgcccag tgccaaaccc ccgccagaca gcctgatgtg cagtgggagc 4201 tcactggtgg cctctgcggg cttggagagc cacggccgga ccccatctcc atccccaccc 4261 tgcagcagct cccccagtcc ctcaggccgg gctggcccgt tctccagcag caggtgcggc 4321 gcctctgtcc ccatcccagt ccccacgcag gtgcagaact accagcgcat tgagcgaaac 4381 ctgcagtcac ccacccagtt ccaaacacct cggtcctctg ccatccgcag gtcaggcagc 4441 accagccccc tgggctttgc aagggccagc ccctcgcccc ctgcccacgc tgagcatgga 4501 ggcgtcctgg ccaggaagat gtctctgggt ggaggccggc cctacacgcc atctcctcaa 4561 gttggaacca tccctgagcg gccaggctgg agcgggacgc cctccccaca gggagctgag 4621 atgcggggtg gcaggtcccc tcgtccaggc tcctctgcac ccgagcactc tccccgcact 4681 tccgggctgg gctgccgcct gcacagcgcc cccaacctgt ctgacttgca cgtcgtccgc 4741 cccaagctgc ccaaaccccc cacggacccc ctgggagctg tgttcagccc accacaggcc 4801 agccctcccc agccgtccca cggcctgcag tcctgccgga acctgcgggg ctcacccaag 4861 ctgcccgact tcctgcagcg aaaccccctg ccccccatcc tgggctcccc caccaaggct 4921 gtgccctcct ttgacttccc gaagaccccc agctcccaga acctgctggc cctcctagcc 4981 cggcagggcg tggtgatgac gccccctcga aaccggacgc tgcccgacct ctcggaggtg 5041 ggacccttcc atggtcagcc gttgggccct ggcctgcggc caggcgagga ccccaagggc 5101 ccctttggcc ggtctttcag caccagccgc ctcactgacc tgctccttaa ggcggcgttt 5161 gggacacaag ccccggaccc gggcagcacg gagagcctgc aggagaagcc catggagatc 5221 gcaccctcag ctggctttgg agggagcctg cacccaggag cccgtgctgg gggcaccagc 5281 agcccttccc cggtggtctt caccgtgggc tctcccccga gcgggagcac gcccccccag 5341 ggcccccgca ccaggatgtt ctcagcgggc cccactggct ctgccagctc ttctgcccgc 5401 cacctggtgc ctgggccctg cagcgaggcc ccagcccctg agctccctgc tccaggacac 5461 ggctgcagct ttgccgaccc cattactgcg aacctggagg gggctgtgac cttcgaggcc 5521 cccgacctcc ctgaggagac cctcatggag caagagcaca cggagatcct gcgtggcctg 5581 cgcttcacgc tgctgttcgt gcagcacgtc ctggagatcg cagccctgaa gggcagcgcc 5641 agtgaggcgg cggggggccc tgagtaccag ctgcaggaga gtgtggtggc cgaccagatc 5701 agcctgctga gccgagaatg gggcttcgcg gaacagctgg tgctgtacct gaaggtggcc 5761 gagctactgt cctccggcct gcaaagtgcc atcgaccaga tccgggccgg caagctctgc 5821 ctgtcgtcca ctgtgaagca ggtggtgcgc aggctgaatg agctgtacaa ggccagcgtg 5881 gtgtcctgcc agggcctgag cctgcggctg cagcgcttct tcctggacaa gcagcggctc 5941 ctggaccgca ttcacagcat cactgccgag aggctcatct tcagccacgc tgtgcagatg 6001 gtgcagtcgg ctgccctgga cgagatgttc cagcaccgtg agggctgcgt cccacgctac 6061 cacaaggccc tgctgctcct ggaggggctg cagcacatgc tctcggacca ggccgacatc 6121 gagaacgtca ccaagtgcaa gctgtgcatt gagcggagac tctcggcgct gctgactggc 6181 atctgtgcct gaGCGGCCGC GAAGGATCTG CGATCGCTCC GGTGCCCGTC AGTGGGCAGA 6241 GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACGGGTGC 6301 CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCTTTT 6361 TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCCGTGAACG TTCTTTTTCG 6421 CAACGGGTTT GCCGCCAGAA CACAGCTGAA GCTTCGAGGG GCTCGCATCT CTCCTTCACG 6481 CGCCCGCCGC CCTACCTGAG GCCGCCATCC ACGCCGGTTG AGTCGCGTTC TGCCGCCTCC 6541 CGCCTGTGGT GCCTCCTGAA CTGCGTCCGC CGTCTAGGTA AGTTTAAAGC TCAGGTCGAG 6601 ACCGGGCCTT TGTCCGGCGC TCCCTTGGAG CCTACCTAGA CTCAGCCGGC TCTCCACGCT 6661 TTGCCTGACC CTGCTTGCTC AACTCTACGT CTTTGTTTCG TTTTCTGTTC TGCGCCGTTA 6721 CAGATCCAAG CTGTGACCGG CGCCTACGCT AGATGACCGA GTACAAGCCC ACGGTGCGCC 6781 TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT 6841 ACCCCGCCAC GCGCCACACC GTCGACCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC 6901 AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG 6961 GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG 7021 AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG 7081 AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG 7141 TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG 7201 CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT 7261 TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA 7321 CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GAGTCGACAA TCAACCTCTG GATTACAAAA 7381 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 7441 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 7501 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 7561 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 7621 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 7681 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 7741 TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 7801 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 7861 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC 7921 GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GTACCTTTAA GACCAATGAC TTACAAGGCA 7981 GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC 8041 CAACGAAAAT AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 8101 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 8161 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 8221 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT 8281 CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA 8341 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 8401 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC 8461 TAGCTATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA 8521 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT 8581 GAGGAGGCTT TTTTGGAGGC CTAGACTTTT GCAGAGACGG CCCAAATTCG TAATCATGGT 8641 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 8701 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 8761 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 8821 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 8881 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 8941 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 9001 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 9061 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 9121 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 9181 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 9241 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 9301 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 9361 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 9421 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 9481 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 9541 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 9601 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 9661 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 9721 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 9781 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 9841 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 9901 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 9961 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 10021 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 10081 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 10141 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 10201 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 10261 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 10321 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 10381 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 10441 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 10501 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 10561 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 10621 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 10681 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 10741 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG 10801 AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC 10861 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 10921 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 10981 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 11041 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 11101 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 11161 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 11221 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.