pLV3-CMV-PLSCR3-3×FLAG-CopGFP-

pLV3-CMV-PLSCR3-3×FLAG-CopGFP-Puro plasmid

PVT107585

pLV3-CMV-PLSCR3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PLSCR3(human)-3×FLAG-CopGFP-Puro
Alias: NM_020360.4
Gene lenght: 885
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PLSCR3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-PLSCR3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PLSCR3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg caggctactt gccccccaaa 2341 ggctacgccc cttcgccccc acctccctac cctgtcaccc ctgggtaccc ggagccggcg 2401 ctacatcctg ggcccgggca ggcgccagtg cccgcccagg tacctgcccc agctcccggc 2461 ttcgccctct tcccctcgcc tggccccgtg gccttggggt ctgctgcccc cttcttgcca 2521 ctgccagggg tgccttctgg cctcgaattc ctggtgcaga ttgatcagat tttgattcac 2581 cagaaggctg agcgagtgga aacgttccta ggctgggaga cctgtaatcg gtatgaactg 2641 cgctctgggg ccgggcagcc cctgggtcag gcggccgagg agagcaactg ctgcgcccgt 2701 ctgtgctgtg gcgcccgccg gccgctgcgt gtccgcctgg ccgaccccgg ggaccgtgag 2761 gtgctgcgtt tgctccgccc gctgcactgt ggctgcagct gctgcccctg tggcctccag 2821 gagatggaag tacaggctcc accaggcacc accattggcc acgtgctaca gacctggcat 2881 cccttcctcc ccaagttctc catccaggat gccgatcgcc agacagtctt gcgagtggtg 2941 gggccctgct ggacctgtgg ctgtggcaca gacaccaact ttgaggtgaa gactcgggat 3001 gaatcccgca gtgtgggccg catcagcaag cagtgggggg gcctggtccg agaagccctc 3061 acagatgcag atgactttgg cctacagttc ccgctggacc tggatgtgag ggtgaaggct 3121 gtgctgctgg gagccacatt cctcattgac tacatgttct ttgagaagcg aggaggcgct 3181 gggccctctg ccgtcaccag tggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3241 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 3301 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 3361 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 3421 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 3481 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 3541 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 3601 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 3661 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 3721 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 3781 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 3841 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 3901 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 3961 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 4021 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 4081 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 4141 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 4201 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 4261 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 4321 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 4381 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 4441 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 4501 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 4561 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 4621 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 4681 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 4741 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 4801 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 4861 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 4921 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 4981 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 5041 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5101 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5161 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5221 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 5281 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5341 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 5401 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 5461 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 5521 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 5581 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 5641 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 5701 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 5761 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 5821 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 5881 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 5941 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6001 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6061 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6121 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6181 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 6241 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 6301 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 6361 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 6421 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 6481 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 6541 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 6601 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 6661 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6721 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 6781 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6841 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 6901 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 6961 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7021 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7081 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 7141 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7201 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7261 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 7321 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 7381 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 7441 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7501 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7561 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7621 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7681 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7741 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 7801 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 7861 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 7921 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 7981 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 8041 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 8101 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 8161 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 8221 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 8281 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 8341 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 8401 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 8461 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 8521 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8581 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8641 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 8701 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 8761 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 8821 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 8881 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 8941 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 9001 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 9061 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 9121 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.