pLV3-CMV-ARAP3-3×FLAG-Puro pla

pLV3-CMV-ARAP3-3×FLAG-Puro plasmid

PVT107471

pLV3-CMV-ARAP3-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-ARAP3(human)-3×FLAG-Puro
Alias: NM_022481.6;CENTD3; DRAG1
Gene lenght: 4632
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F(HighGC）
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ARAP3-3×FLAG-Puro plasmid Description

pLV3-CMV-ARAP3-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-ARAP3-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggctgcc cctcaggacc tggacatcgc tgtgtggctg 2341 gccacggtgc acctggagca gtatgcagac acgttccgac ggcatggcct ggctacagca 2401 ggtgcagccc ggggcctggg ccacgaggag ttgaagcagt tgggcatcag cgccacaggg 2461 caccggaaac gcattctacg cctgctacag acaggcaccg aagagggctc cctggatccc 2521 aaatcagata gtgccatgga accatccccc agcccagccc cgcaagccca gccccctaag 2581 cccgtgccga agcccaggac cgtgtttggt ggactcagtg gccctgccac cactcagaga 2641 cctgggctga gcccagccct cgggggacca ggagtgtcca ggagcccaga gcccagccca 2701 aggcctcctc ctctccccac ttcctcctct gagcagtctt cagccctaaa tactgtggag 2761 atgatgccta attccatcta cttcggcctg gactcaagag gcagggcaca ggcagctcag 2821 gacaaggccc cagacagctc ccaaatctct gcccccaccc ctgccctcag gcccacaaca 2881 ggcacagtgc acatcatgga tcctggttgc ctgtactatg gtgtccaacc tgtggggact 2941 ccaggagccc ccgacagaag agagagcaga ggtgtttgtc agggcagggc tgaacacagg 3001 ctcagcagac aggatctgga ggcacgggag gatgctggct atgccagcct tgagctacct 3061 ggagactcca ccctcttatc gcccaccctg gaaacagagg agaccagtga tgacctcatt 3121 tcaccctatg ccagcttctc cttcacggca gaccgcctca cgcccctgct cagtggctgg 3181 ctagacaagc tctcccctca gggaaactat gtcttccaga gacgctttgt gcagttcaat 3241 gggaggagtc tgatgtactt tggcagtgac aaggacccct tccctaaggg tgtgatacct 3301 ttgactgcca ttgagatgac ccgcagcagc aaggacaaca agttccaggt catcaccggc 3361 cagagggtgt tcgtgttccg cacagagagc gaggctcagc gggacatgtg gtgctccacg 3421 ctgcagtcct gtctgaagga gcagcgcctc ctgggccacc cccggccccc ccaaccaccc 3481 cgacccctcc gcacgggcat gctggagctg cgtggacaca aggccaaggt gtttgctgcc 3541 ttgagccctg gagagctggc actgtacaag agtgagcagg ccttctctct gggcatcggg 3601 atctgcttca tcgaactgca gggctgcagc gtccgggaga ccaagagtcg aagctttgac 3661 ctgctcacac cccatcgctg cttcagcttc acagccgagt ctgggggtgc tcggcagagc 3721 tgggcggccg ctctgcagga agcagtaacc gagaccctgt ctgactacga ggtggctgag 3781 aagatctggt ctaatcgggc caaccggcag tgtgcggact gtgggtcctc ccgcccagat 3841 tgggctgctg tcaatttggg ggtggtcatc tgcaagcagt gtgcaggtca gcaccgggcc 3901 ctgggttctg ggatctccaa ggtgcagagc ctgaagctgg acacgagtgt ctggagtaat 3961 gagatagtac agttattcat tgtcctggga aatgatcgtg ccaaccgctt ctgggcaggg 4021 accctacccc caggtgaggg actacatcca gatgcgaccc ctggcccccg gggagagttc 4081 atctcccgaa agtaccgtct gggtctcttc cggaagcccc accctcagta cccagatcat 4141 agccagcttc tccaggcact gtgtgcagct gtggcaagac ccaacctgct gaagaacatg 4201 acccagctcc tctgtgttga ggcctttgaa ggcgaggagc cctggttccc cccagcccct 4261 gatggcagct gccctggcct cttgccctca gacccctccc ctggtgtgta caatgaggtg 4321 gtggtgcgtg ctacttacag cggcttcctg tactgcagtc ccgtcagcaa caaagctgga 4381 ccctcacccc ctcgcagggg ccgggatgct cccccgcgcc tttggtgtgt gctgggagca 4441 gctctggaaa tgtttgcatc ggaaaacagc cctgaacccc tcagcctcat acagccccag 4501 gatattgtat gtctgggtgt gagcccccca cccactgacc caggtgacag gttccccttt 4561 tcctttgagc tcatcctcgc tggggggagg atccagcatt ttggcacaga tggagctgac 4621 agtctggagg cctggactag tgctgtgggc aagtggttct ccccgctgag ctgccaccag 4681 ctgctgggcc ccgggctgct gcggctgggc cgcctatggc tgcggtcccc ctcccataca 4741 gccccggccc ctggtctctg gctgtcaggg tttggcctcc ttcgtggtga ccacctcttc 4801 ctgtgctcag cgccgggccc aggcccccca gcccctgagg acatggtgca tctgcggcgg 4861 ctacaggaga tcagtgtggt ttctgcagct gacaccccag ataagaaaga gcatttggtc 4921 ctggtggaga caggaaggac cctgtatctg caaggagagg gccggctgga cttcacggca 4981 tggaacgcag ccattggggg cgcggctggt gggggcggca cagggctgca ggagcagcag 5041 atgagccggg gtgacatccc catcatcgtg gatgcctgca tcagttttgt tacccagcat 5101 gggctccggc tggaaggtgt ataccggaaa gggggcgctc gtgcccgcag cctgagactc 5161 ctggctgagt tccgtcggga tgcccggtcg gtgaagctcc gaccagggga gcactttgtg 5221 gaggatgtca ctgacacact caaacgcttc tttcgtgagc tcgatgaccc tgtgacctct 5281 gcacggttgc tgcctcgctg gagggaggct gctgagctgc cccagaagaa tcagcgcctg 5341 gagaaatata aagatgtgat tggctgcctg ccgcgggtca accgccgcac actggccacc 5401 ctcattgggc atctctatcg ggtgcagaaa tgtgcggctc taaaccagat gtgcacgcgg 5461 aacttggctc tgctgtttgc acccagcgtg ttccagacgg atgggcgagg ggagcacgag 5521 gtgcgagtgc tgcaagagct cattgatggc tacatctctg tctttgatat cgattctgac 5581 caggtagctc agattgactt ggaggtcagt cttatcacca cctggaagga cgtgcagctg 5641 tctcaggctg gagacctcat catggaagtt tatatagagc agcagctccc agacaactgt 5701 gtcaccctga aggtgtcccc aaccctgact gctgaggagc tgactaacca ggtactggag 5761 atgcggggga cagcagctgg gatggacttg tgggtgactt ttgagattcg cgagcatggg 5821 gagctggagc ggccactgca tcccaaggaa aaggtcttag agcaggcttt acaatggtgc 5881 cagctcccag agccctgctc agcttccctg ctcttgaaaa aagtccccct ggcccaagct 5941 ggctgcctct tcacaggtat ccgacgtgag agcccacggg tggggctgtt gcggtgtcgt 6001 gaggagccac ctcgcttgct gggaagccgc ttccaggaga ggttctttct gctgcgtggc 6061 cgctgcctgc tgctgctcaa ggagaagaaa agctctaaac cagaacggga gtggcctttg 6121 gaaggtgcca aggtctacct gggaatccgc aagaagttaa agcccccaac accgtggggc 6181 ttcacattga tactagagaa gatgcacctc tacttgtcct gcactgacga ggatgaaatg 6241 tgggattgga ccaccagcat ccttaaagcc cagcacgatg accagcagcc agtggtctta 6301 cgacgccatt cctcctctga ccttgcccgt cagaagtttg gcactatgcc tttgctgcct 6361 atccgtgggg atgacagtgg agccaccctc ctctctgcca atcagaccct gcggcgacta 6421 cacaaccgga ggaccctgtc catgttcttt ccaatgaagt catcccaggg gtctgtggag 6481 gagcaagagg agctggagga gcctgtgtac gaggagccag tgtatgagga agtaggggcc 6541 ttccctgagt tgatccagga cacttctacc tccttctcca ccacacggga gtggacagtg 6601 aagccagaga accccctcac cagccagaag tcattggatc aaccctttct ctccaagtca 6661 agcacccttg gccaggagga gaggccacct gagccccctc caggcccccc ttcaaagagc 6721 agtccccagg cacgggggtc cctagaggaa cagctgctcc aggagctcag cagcctcatc 6781 ctgaggaaag gagagaccac tgcaggcctg ggaagtcctt cccagccatc cagcccccaa 6841 tcccccagcc ccactggcct tccaacacag acacctggct tccccaccca acccccatgc 6901 acttccagtc caccctccag ccagcccctc acaGGATCCG ACTACAAAGA CCATGACGGT 6961 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAGCG GCCGCGAAGG 7021 ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG 7081 AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC 7141 TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT 7201 ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG 7261 CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC GCCGCCCTAC CTGAGGCCGC 7321 CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT GTGGTGCCTC CTGAACTGCG 7381 TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG GCCTTTGTCC GGCGCTCCCT 7441 TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC TGACCCTGCT TGCTCAACTC 7501 TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT CCAAGCTGTG ACCGGCGCCT 7561 ACGCTAGATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCCG 7621 GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA 7681 CCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG 7741 GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC 7801 GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT 7861 GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC 7921 CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG 7981 TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC 8041 CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT 8101 CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG 8161 TGCCTGAGTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT 8221 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC 8281 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT 8341 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA 8401 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC 8461 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC 8521 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT 8581 TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT 8641 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC 8701 TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC 8761 GCCTGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA 8821 AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAAATAAGAT CTGCTTTTTG 8881 CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG 8941 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC 9001 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA 9061 TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT 9121 GAATATCAGA GAGTGAGAGG AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 9181 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 9241 CCAAACTCAT CAATGTATCT TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC 9301 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 9361 GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGA 9421 CTTTTGCAGA GACGGCCCAA ATTCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 9481 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 9541 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 9601 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 9661 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 9721 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 9781 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 9841 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 9901 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 9961 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 10021 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 10081 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 10141 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 10201 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 10261 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT 10321 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 10381 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 10441 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 10501 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 10561 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 10621 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 10681 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 10741 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 10801 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 10861 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 10921 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 10981 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 11041 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 11101 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 11161 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 11221 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 11281 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 11341 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 11401 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 11461 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 11521 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 11581 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC 11641 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA 11701 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 11761 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA 11821 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA 11881 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG 11941 TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG 12001 TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC 12061 GACGGCCAGT GCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.