pLV3-EF1a-Anxa1-3×FLAG-PGK-Cop

pLV3-EF1a-Anxa1-3×FLAG-PGK-CopGFP-Puro plasmid

PVT107444

pLV3-EF1a-Anxa1-3×FLAG-PGK-CopGFP-Puro plasmid Information

Plasmid name: pLV3-EF1a-Anxa1(rat)-3×FLAG-PGK-CopGFP-Puro
Alias: NM_012904.2;Anx1; p35
Gene lenght: 1038
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-Anxa1-3×FLAG-PGK-CopGFP-Puro plasmid Description

pLV3-EF1a-Anxa1-3×FLAG-PGK-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-EF1a-Anxa1-3×FLAG-PGK-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cGCCACCatg gcaatggtat cagaattcct 2521 caagcaggcc tgctatattg aaaagcaaga gcaggaatat gttcaagctg taaaatccta 2581 caaaggtggt cctggatcag cagtgagccc ctacccttcc ttcaatccgt cctcggatgt 2641 tgctgccttg cacaaagcta tcatggttaa aggtgtggat gaggcaacca tcattgacat 2701 ccttaccaag agaaccaatg ctcagcgcca gcagatcaag gcagcatact tacaggagac 2761 tgggaagccc ctggatgaaa ccttgaaaaa agcccttacg ggccacctgg aggaggttgt 2821 tttggctatg ctcaagaccc cagctcagtt tgatgcagat gaactccgtg ctgccatgaa 2881 gggacttgga acagatgaag acactctcat tgagattttg acaacaagat ctaaccagca 2941 aatcagagag attactagag tctacagaga agagctgaaa agagatctgg ccaaagacat 3001 cacttcggac acatctggag actttcgtaa tgccttgctt gctctcgcca agggtgatcg 3061 ctgtgaggat atgagtgtga atcaagattt ggctgataca gatgccaggg ctttgtatga 3121 agctggagaa aggagaaagg ggacagacgt gaatgtgttc aatacaattt tgaccacaag 3181 aagctatcct catcttcgga aagtgtttca gaattataga aagtacagtc aacatgacat 3241 gaacaaagcc ctggatctgg aactgaaggg tgacattgag aagtgcctca caaccattgt 3301 gaagtgtgcc accagcactc cagctttctt tgctgagaaa ctgtatgaag ccatgaaggg 3361 tgctggaact cgccataaga cattgatcag gattatggtc tcccgttcgg aaattgacat 3421 gaatgaaatc aaagtatttt accagaagaa gtacggaatc cctctctgcc aagccatcct 3481 ggatgaaacc aaaggagact atgaaaaaat cctggtggct ctgtgtggag gaaacggatc 3541 cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA 3601 TGACAAGtag cggccgctgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg 3661 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt 3721 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt 3781 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt 3841 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat 3901 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg 3961 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg 4021 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg 4081 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat 4141 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct 4201 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat 4261 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca 4321 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt 4381 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg 4441 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt 4501 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg 4561 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt 4621 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat 4681 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt 4741 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc 4801 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga 4861 cggcaccgcc ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg 4921 cggtgacgtg gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc 4981 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 5041 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 5101 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 5161 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 5221 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 5281 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 5341 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 5401 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 5461 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 5521 gtgcatgacc cgcaagcccg gtgcctgagt cgacaatcaa cctctggatt acaaaatttg 5581 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 5641 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 5701 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 5761 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 5821 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 5881 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 5941 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 6001 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 6061 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 6121 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg 6181 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 6241 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 6301 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 6361 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 6421 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 6481 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 6541 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 6601 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 6661 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 6721 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 6781 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata 6841 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6901 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6961 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 7021 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7081 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7141 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7201 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7261 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7321 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7381 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7441 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7501 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7561 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7621 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7681 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7741 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7801 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7861 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7921 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7981 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 8041 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 8101 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8161 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8221 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8281 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8341 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8401 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8461 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8521 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8581 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8641 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8701 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8761 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8821 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8881 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8941 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 9001 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 9061 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 9121 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9181 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9241 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9301 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9361 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9421 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.