pCMV-mCherry-FYCO1-3×HA-Neo pl

pCMV-mCherry-FYCO1-3×HA-Neo plasmid

PVT107403

pCMV-mCherry-FYCO1-3×HA-Neo plasmid Information

Plasmid name: pCMV-mCherry-FYCO1(human)-3×HA-Neo
Alias: NM_024513.4;CATC2; CTRCT18; RUFY3; ZFYVE7
Gene lenght: 4434
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: MCHERRY-F
Reverse primer:
Copy number: High
Induction:

pCMV-mCherry-FYCO1-3×HA-Neo plasmid Description

pCMV-mCherry-FYCO1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-mCherry-FYCO1-3×HA-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgaGG AGGAGGGGGA AGCgaagcta tggcctccac caatgcagag 1381 agccagctcc agagaatcat ccgagacttg caagatgctg tgacagaact aagcaaagaa 1441 tttcaggaag caggggaacc catcacggat gacagcacca gcttgcataa attttcttat 1501 aaacttgagt atctcctgca atttgatcag aaagagaagg ccaccctcct gggcaacaag 1561 aaggactact gggattactt ctgtgcctgc ctggccaagg tgaaaggagc caatgatggg 1621 atccgctttg tcaagtctat ctcagagctc cgaacatcct tggggaaagg aagagcattt 1681 attcgctact ccttggtgca ccagaggttg gcagacacct tacagcagtg cttcatgaac 1741 accaaagtga ccagtgactg gtactatgca agaagcccct ttctgcagcc aaagctgagc 1801 tcggacattg tgggccaact ctatgagctg actgaggttc agtttgacct ggcgtcgagg 1861 ggctttgact tggatgctgc ctggccaaca tttgccagga ggacgctgac cactggctct 1921 tctgcttacc tgtggaaacc ccctagccgc agctccagca tgagcagctt ggtgagcagc 1981 tacctgcaga ctcaagagat ggtgtccaac tttgacctga acagccccct aaacaacgag 2041 gcattggagg gctttgatga gatgcgacta gagctggacc agttggaggt gcgggagaag 2101 cagctacggg agcgcatgca gcagctggac agagagaacc aggagctgag ggcagctgtc 2161 agccagcaag gggagcaact gcagacagag agggagaggg ggcgcactgc agcggaggac 2221 aacgttcgcc tcacttgctt ggtagctgag ctccagaagc agtgggaggt cacccaggcc 2281 acccagaaca ctgtgaagga gctgcagaca tgcctgcagg gcctggagct aggagcagca 2341 gagaaggagg aggactacca cacagccctg cggcggctgg agtccatgct gcagcccttg 2401 gcacaggagc ttgaggccac acgggactca ctggacaaga aaaaccagca tttagccagc 2461 ttcccaggct ggctagccat ggctcagcag aaggcagata cggcatcaga cacaaagggc 2521 cggcaagaac ctattcccag tgatgcggcc caggagatgc aggagctagg ggagaagctt 2581 caagccctag aaagggagag aaccaaggtc gaggaggtca acagacagca gagtgcccaa 2641 ctggaacagc tggtcaagga gcttcagctg aaagaggatg cccgggccag cctggagcgc 2701 ctggtgaagg agatggcccc actccaggag gagttgtctg ggaagggaca ggaggcagac 2761 cagctctggc gacggctgca ggagttgctg gcccacacga gctcctggga ggaggagcta 2821 gcagagttga ggcgggagaa aaaacagcaa caggaggaga aggagctgct ggagcaggag 2881 gtcaggtctc tgacccggca gctgcagttc ctggagaccc agctggcaca ggtgagccaa 2941 catgtgagtg acctggagga gcagaagaag cagctcattc aggacaaaga ccacctcagc 3001 cagcaggtgg gtatgctcga gcggcttgct gggccgcctg gcccagaact gccagtggca 3061 ggtgagaaga atgaggccct ggtccctgtg aactccagtc tgcaagaggc ctgggggaag 3121 ccagaggagg agcagagggg cctgcaggag gcacagttag acgataccaa ggtgcaagag 3181 ggcagccagg aggaagagct ccggcaggcc aacagggagc tggagaagga gctacagaat 3241 gtggtcgggc gtaaccagct cctggagggc aagctgcaag ccctgcaggc cgattaccag 3301 gctttgcagc agcgggaatc agccatccag ggctccttgg cctccctgga ggccgagcag 3361 gccagcatcc ggcacttggg tgaccagatg gaggcgagct tgctggctgt aaggaaggcc 3421 aaggaggcca tgaaagccca gatggcagag aaggaggcca ttctacagag caaggagggc 3481 gagtgtcagc agctgcggga ggaggtggag cagtgccagc aactggcaga agcccggcac 3541 agagagctta gggctctcga gagccagtgc cagcagcaga cccagctgat tgaggtcctc 3601 acagcagaga aaggccaaca gggagttggc ccacccactg acaatgaagc ccgtgagctg 3661 gctgcccagc tagccctgtc tcaggcgcag ctggaagtcc atcaggggga ggtccaacgg 3721 ctgcaggctc aggtggtgga cctccaggcc aagatgcggg cagccctgga tgaccaggac 3781 aaggtgcaga gccagctaag catggctgag gccgtcctga gggagcacaa aacccttgtg 3841 cagcagctga aggagcagaa tgaagccctt aacagagccc atgtccagga gctgctgcaa 3901 tgctcggagc gtgaaggggc actgcaggag gagagggccg atgaggccca gcagagggag 3961 gaggagctgc gggccctgca ggaggagctg tcccaggcca aatgcagctc cgaggaagca 4021 cagctggagc acgctgagct gcaagagcag ctgcaccggg ccaacacaga cacagctgag 4081 ctgggcatcc aggtttgcgc actgaccgtg gaaaaggagc gagtggagga ggcactggcc 4141 tgtgctgtcc aggagctcca ggacgccaaa gaggcagcct caagggagcg agagggcctg 4201 gagcgccaag tagctgggct gcagcaagag aaggagagct tgcaggagaa gctgaaggcg 4261 gccaaggcag cagccggctc actgcctggc ctgcaggccc agctcgccca ggcagagcag 4321 cgggcccaga gcctccaaga ggctgcacac caggagctca acaccctcaa gttccagctg 4381 agtgctgaaa tcatggacta ccagagcaga cttaagaatg ctggtgaaga gtgcaagagc 4441 ctcaggggcc agcttgagga gcaaggccgg cagctgcagg ctgctgagga agctgtggag 4501 aagctgaagg ccacccaagc agacatggga gagaagctga gctgcactag caaccatctt 4561 gcagagtgcc aggcggccat gctgaggaag gacaaggagg gggctgccct gcgtgaagac 4621 ctagaaagga cccagaagga actcgaaaaa gccacaacaa aaatccaaga gtattacaac 4681 aaactctgcc aggaggtgac aaatcgtgag aggaatgacc agaagatgct tgctgacctg 4741 gatgacctca acagaaccaa gaagtatctc gaggagcggc tgatagagct gctcagggac 4801 aaggatgctc tctggcagaa gtcagatgcc ctggaattcc agcagaagct cagtgctgag 4861 gagagatggc tcggagacac agaggcaaac cactgcctcg actgtaagcg ggagttcagc 4921 tggatggtgc ggcggcacca ctgcaggata tgtggccgca tcttctgtta ctactgctgc 4981 aacaactacg tcctgagcaa gcacggtggc aaaaaggagc gctgctgccg agcctgtttc 5041 cagaagctca gtgaaggccc tggctcccct gatagcagtg gctcaggcac tagccaggga 5101 gagcccagcc ctgcactgtc accagcctca cctgggcccc aggccacagg aggccaagga 5161 gcaaatacag actacaggcc accggacgac gctgtgtttg atatcatcac agatgaggaa 5221 ttgtgccaga tacaggagtc cggctcctct ttgcctgaaa cacccactga aactgattct 5281 ctcgacccaa atgcggctga acaggatact acatcaacct cgctaacgcc tgaggacact 5341 gaagacatgc ccgtggggca ggattcggaa atctgcctgc tgaagtctgg agaactgatg 5401 atcaaagtac ccctcacagt ggatgagatc gccagcttcg gggagggtag cagggagctg 5461 tttgtgaggt ccagcaccta cagcctgatc cccatcactg tggccgaggc aggcctcacc 5521 atcagctggg tcttctcctc tgaccccaag agcatctcct tcagtgtggt cttccaggag 5581 gccgaggaca caccgctgga tcagtgtaag gtcctcattc ccacgacccg atgcaactcc 5641 cacaaggaga acatccaggg ccagctcaag gttcgcacac ccggcatcta catgctcatc 5701 ttcgacaata ccttctcaag gtttgtctct aaaaaggtat tttatcactt gacggttgat 5761 cggcctgtga tctacgatgg aagtgatttc ctgggatccT ACCCATACGA CGTACCTGAT 5821 TATGCAACTT ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC 5881 TACGCAtgag atccaccgga tctagataac tgatcataat cagccatacc acatttgtag 5941 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 6001 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 6061 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 6121 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg 6181 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 6241 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 6301 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 6361 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 6421 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 6481 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 6541 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 6601 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 6661 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 6721 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 6781 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 6841 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 6901 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 6961 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 7021 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 7081 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 7141 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 7201 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 7261 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg 7321 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 7381 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 7441 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 7501 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 7561 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 7621 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc 7681 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 7741 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 7801 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 7861 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 7921 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 7981 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 8041 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga 8101 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 8161 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 8221 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 8281 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 8341 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 8401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 8461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 8521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 8581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 8641 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 8701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 8761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 8821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 8881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 8941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 9001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 9061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 9121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 9181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.