pCMV-Crhr1-3×HA-mCMV-EGFP-Neo

pCMV-Crhr1-3×HA-mCMV-EGFP-Neo plasmid

PVT111282

pCMV-Crhr1-3×HA-mCMV-EGFP-Neo plasmid Information

Plasmid name: pCMV-Crhr1(mouse)-3×HA-mCMV-EGFP-Neo
Alias: NM_007762.5;CRF-R1alpha; CRF1R; CRFR1; Crhr
Gene lenght: 1245
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Crhr1-3×HA-mCMV-EGFP-Neo plasmid Description

pCMV-Crhr1-3×HA-mCMV-EGFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Crhr1-3×HA-mCMV-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCgc caccatggga cagcgcccgc agctccggct 661 cgtgaaggcc cttctccttc tggggctgaa ccccgtctcc acctccctcc aggatcagca 721 gtgtgagagc ctgtccctgg ccagcaatgt ctctggcctg cagtgcaatg cctccgtgga 781 cctcattggc acctgctggc ccaggagccc tgcagggcag ttggtggttc ggccctgccc 841 tgcctttttc tacggtgtcc gctacaacac cacaaacaat ggctaccggg aatgcctggc 901 caacggcagc tgggcagccc gtgtgaatta ttctgagtgc caggagattc tcaacgaaga 961 gaagaagagc aaagtgcact accacattgc cgtcatcatc aactacctgg gccactgcat 1021 ctccctggtg gccctcctgg tggcctttgt cctcttcctg cggctcagga gcatccggtg 1081 cctgaggaac atcatccact ggaacctcat ctcggctttc atcctgcgca acgccacgtg 1141 gtttgtggtc cagctcaccg tgagccccga ggtccaccag agcaacgtgg cctggtgcag 1201 gctggtgaca gccgcctaca actacttcca cgtaaccaac ttcttctgga tgttcggtga 1261 gggctgctac ctgcacacag ccatcgtact cacgtactcc accgaccgtc tgcgcaagtg 1321 gatgttcgtc tgcatcggct ggggtgtgcc tttccccatc attgtggctt gggccattgg 1381 gaaactttac tacgacaatg aaaagtgctg gtttggcaaa cgtcctggag tatatactga 1441 ctacatctac cagggcccca tgatcctggt cctgctgatc aactttatct ttctcttcaa 1501 cattgtccgc atcctcatga ccaaactccg agcatccacc acatctgaga ctattcagta 1561 caggaaggct gtgaaggcca ctctggtgct cttgcccctc ctgggcatca cctacatgtt 1621 gttcttcgtc aaccctgggg aggacgaggt ctccagggtt gtcttcatct acttcaactc 1681 tttcctggag tcctttcagg gcttcttcgt gtctgtgttc tattgttttc tgaacagtga 1741 ggtccgctct gccatccgga agaggtggcg gcgatggcag gacaagcact ccatcagagc 1801 ccgagtggcc cgcgccatgt ccatccccac ctcccccacc agagtcagct tccacagcat 1861 caagcagtcc acagcagtgA AGCTTTACCC ATACGACGTA CCTGATTATG CAACTTATCC 1921 TTATGATGTA CCTGATTATG CTAGCTATCC TTATGATGTC CCAGACTACG CATAGGATCC 1981 GGGCCCGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT 2041 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC 2101 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT 2161 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT 2221 GCGGTGGGCT CTATGGTCTA GAGATATACT GAGTCATTAG GGACTTTCCA ATGGGTTTTG 2281 CCCAGTACAT AAGGTCAATA GGGGTGAATC AACAGGAAAG TCCCATTGGA GCCAAGTACA 2341 CTGAGTCAAT AGGGACTTTC CATTGGGTTT TGCCCAGTAC AAAAGGTCAA TAGGGGGTGA 2401 GTCAATGGGT TTTTCCCATT ATTGGCACGT ACATAAGGTC AATAGGGGTG AGTCATTGGG 2461 TTTTTCCAGC CAaTTTAATT AAAACGCCAT GTACTTTCCC ACCATTGACG TCAATGGGCT 2521 ATTGAAACTA ATGCAACGTG ACCTTTAAAC GGTACTTTCC CATAGCTGAT TAATGGGAAA 2581 GTACCGTTCT CGAGCCAATA CACGTCAATG GGAAGTGAAA GGGCAGCCAA AACGTAACAC 2641 CGCCCCGGTT TTCCCCTGGA AATTCCATAT TGGCACGCAT TCTATTGGCT GAGCTGCGTT 2701 CTACGTGGGT ATAAGAGGCG CGACCAGCGT CGGTACCGTC GCAGTCTTCG GTCTGACCAC 2761 CGTAGAACGC AGATCaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg 2821 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 2881 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 2941 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg 3001 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 3061 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 3121 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 3181 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 3241 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa 3301 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 3361 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 3421 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 3481 tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat 3541 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 3601 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 3661 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 3721 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt 3781 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 3841 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 3901 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 3961 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 4021 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 4081 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 4141 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 4201 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 4261 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 4321 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 4381 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 4441 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 4501 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 4561 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 4621 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 4681 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 4741 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 4801 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 4861 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 4921 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 4981 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 5041 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 5101 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 5161 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 5221 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 5281 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 5341 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 5401 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 5461 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 5521 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 5581 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 5641 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 5701 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 5761 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 5821 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 5881 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 5941 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 6001 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 6061 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 6121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 6181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 6241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 6301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 6361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 6421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 6481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 6541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 6601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 6661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 6721 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 6781 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 6841 cat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.