pLV3-CMV-Pias3-3×FLAG-CopGFP-P

pLV3-CMV-Pias3-3×FLAG-CopGFP-Puro plasmid

PVT107029

pLV3-CMV-Pias3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Pias3(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001165949.1;Pias3l
Gene lenght: 1884
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Pias3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Pias3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-Pias3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg cggagctggg cgagttaaag 2341 cacatggtga tgagtttccg agtgtctgag ctccaggtgc tcctcggctt cgctggcagg 2401 aacaagagtg ggcggaaaca cgagctgctg gccaaggccc tgcacctcct caagtctagc 2461 tgcgccccca gcgtccagat gaagatcaaa gaactttatc gcaggcgctt tccccggaag 2521 accctggggc cttctgatct ctccttgctt tctttgcccc ctggcacctc tcctgtaggc 2581 tcccccggcc cccttgctcc catccctccc acccttctga cccctggcac cttgctaggc 2641 cctaagcgtg aggtggacat gcatcctcct ctgccgcagc ctgtgcaccc cgatgtcacc 2701 atgaagccac tgcccttcta tgaagtctat ggggagctca tccgacccac cacccttgcg 2761 tccacctcca gccagaggtt cgaggaagcc cacttcacct tcgcgctcac tccccagcag 2821 ctgcagcaga ttctcacgtc cagggaggtt ctgccaggag ccaagtgtga ttacaccata 2881 caagtgcagc tcagattctg tctctgtgag accagctgcc ctcaggagga ctatttcccc 2941 cctaacctct ttgttaaggt taatgggaaa ctctgccccc tgccgggtta cctccctcca 3001 accaagaatg gagctgagcc caagaggccc agccgtccga tcaacatcac acccttggct 3061 cgactctcag ccactgtccc caacaccatc gtagttaatt ggtcatctga gtttggacgg 3121 aattactcct tgtccgtgta cctggtgagg caattgactg cagggaccct tctacaaaaa 3181 ctcagagcca aggggatccg gaatccagac cattcccggg cactgatcaa ggagaaactg 3241 actgctgacc ccgacagtga agtggctact acaagtctcc gggtgtcact catgtgcccg 3301 ctagggaaga tgcgcctgac tgtcccgtgt cgtgccctca cctgtgccca tctgcagagt 3361 ttcgatgctg ccctttatct acagatgaat gagaagaagc cgacatggac gtgtcctgtg 3421 tgtgacaaga aggctcccta tgagtcgctg attattgatg gtttattcat ggaaattctt 3481 aattcctgtt cggattgtga tgagatccag ttcatggaag atggatcctg gtgtccgatg 3541 aaacccaaga aggaggcatc agaggtttgc cccccgccag ggtatgggct ggatggtctc 3601 cagtacagcg cagtccagga gggaattcag ccagagagta agaagagggt cgaagtcatt 3661 gacttgacca tcgaaagctc atcagatgag gaggatttgc cccccaccaa gaagcactgc 3721 cctgtcacct cagcggccat tccagccctt cctggaagca aaggagccct gacctctggt 3781 caccagccat cctcggtgct gcggagccct gcaatgggca cactgggcag tgacttcctg 3841 tctagtctcc cgctacatga gtacccacct gccttcccac tgggggctga catccaaggt 3901 ttagatttat tttctttcct tcagactgag agtcagcact acggcccttc agttatcact 3961 tcgctagatg aacaggacac ccttggccac ttcttccagt accggggaac cccttcccac 4021 ttcctgggcc cactggcccc cacactgggg agctctcacc gcagctccac tccagcgccc 4081 cctcctggtc gtgtcagcag cattgtggct cctgggagct ccttgaggga agggcatgga 4141 ggacccctgc cttcaggtcc ctctttgact ggctgtcggt cagacgtcat ttccttggac 4201 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 4261 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 4321 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 4381 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 4441 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 4501 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 4561 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 4621 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 4681 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 4741 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 4801 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 4861 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 4921 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 4981 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 5041 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 5101 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 5161 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 5221 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 5281 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 5341 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 5401 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 5461 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 5521 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 5581 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 5641 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 5701 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 5761 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 5821 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 5881 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 5941 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 6001 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 6061 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 6121 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 6181 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 6241 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 6301 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 6361 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 6421 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 6481 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 6541 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 6601 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 6661 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 6721 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 6781 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 6841 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 6901 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 6961 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 7021 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 7081 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 7141 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 7201 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 7261 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 7321 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 7381 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 7441 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 7501 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 7561 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 7621 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 7681 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 7741 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 7801 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7861 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 8041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 8101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 8161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 8221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 8281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 8341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 8401 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 8461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 8521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 8581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 8641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 8701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 8761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 8821 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 8881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 8941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 9001 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 9061 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 9121 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 9181 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 9241 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 9301 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 9361 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 9421 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 9481 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 9541 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 9601 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 9661 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 9721 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 9781 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 9841 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 9901 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 9961 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 10021 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 10081 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 10141 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.