pUC57-DSP plasmid

pUC57-DSP plasmid

PVT106882

pUC57-DSP plasmid Information

Plasmid name: pUC57-DSP(human)
Alias: NM_001008844.3;DCWHKTA; DP
Gene lenght: 6816
Host: E.coli
Use(s): Gene Template
Fragment type: cDNA
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Non
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: PUC57-F1
Reverse primer:
Copy number: High
Induction:

pUC57-DSP plasmid Description

pUC57-DSP is a plasmid suitable for gene templates in Escherichia coli.

pUC57-DSP plasmid Sequence

1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC 1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 1861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 1921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 1981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 2041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 2101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 2161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 2221 ACCATGATTA CGCCAAGCTT GCATGCAGGC CTCTGCAGTC GACGGGCCCG GGATCCatga 2281 gctgcaacgg aggctcccac ccgcggatca acactctggg ccgcatgatc cgcgccgagt 2341 ctggcccgga cctgcgctac gaggtgacca gcggcggcgg gggcaccagc aggatgtact 2401 attctcggcg cggcgtgatc accgaccaga actcggacgg ctactgtcaa accggcacga 2461 tgtccaggca ccagaaccag aacaccatcc aggagctgct gcagaactgc tccgactgct 2521 tgatgcgagc agagctcatc gtgcagcctg aattgaagta tggagatgga atacaactga 2581 ctcggagtcg agaattggat gagtgttttg cccaggccaa tgaccaaatg gaaatcctcg 2641 acagcttgat cagagagatg cggcagatgg gccagccctg tgatgcttac cagaaaaggc 2701 ttcttcagct ccaagagcaa atgcgagccc tttataaagc catcagtgtc cctcgagtcc 2761 gcagggccag ctccaagggt ggtggaggct acacttgtca gagtggctct ggctgggatg 2821 agttcaccaa acatgtcacc agtgaatgtt tggggtggat gaggcagcaa agggcggaga 2881 tggacatggt ggcctggggt gtggacctgg cctcagtgga gcagcacatt aacagccacc 2941 ggggcatcca caactccatc ggcgactatc gctggcagct ggacaaaatc aaagccgacc 3001 tgcgcgagaa atctgcgatc taccagttgg aggaggagta tgaaaacctg ctgaaagcgt 3061 cctttgagag gatggatcac ctgcgacagc tgcagaacat cattcaggcc acgtccaggg 3121 agatcatgtg gatcaatgac tgcgaggagg aggagctgct gtacgactgg agcgacaaga 3181 acaccaacat cgctcagaaa caggaggcct tctccatacg catgagtcaa ctggaagtta 3241 aagaaaaaga gctcaataag ctgaaacaag aaagtgacca acttgtcctc aatcagcatc 3301 cagcttcaga caaaattgag gcctatatgg acactctgca gacgcagtgg agttggattc 3361 ttcagatcac caagtgcatt gatgttcatc tgaaagaaaa tgctgcctac tttcagtttt 3421 ttgaagaggc gcagtctact gaagcatacc tgaaggggct ccaggactcc atcaggaaga 3481 agtacccctg cgacaagaac atgcccctgc agcacctgct ggaacagatc aaggagctgg 3541 agaaagaacg agagaaaatc cttgaataca agcgtcaggt gcagaacttg gtaaacaagt 3601 ctaagaagat tgtacagctg aagcctcgta acccagacta cagaagcaat aaacccatta 3661 ttctcagagc tctctgtgac tacaaacaag atcagaaaat cgtgcataag ggggatgagt 3721 gtatcctgaa ggacaacaac gagcgcagca agtggtacgt gacgggcccg ggaggcgttg 3781 acatgcttgt tccctctgtg gggctgatca tccctcctcc gaacccactg gccgtggacc 3841 tctcttgcaa gattgagcag tactacgaag ccatcttggc tctgtggaac cagctctaca 3901 tcaacatgaa gagcctggtg tcctggcact actgcatgat tgacatagag aagatcaggg 3961 ccatgacaat cgccaagctg aaaacaatgc ggcaggaaga ttacatgaag acgatagccg 4021 accttgagtt acattaccaa gagttcatca gaaatagcca aggctcagag atgtttggag 4081 atgatgacaa gcggaaaata cagtctcagt tcaccgatgc ccagaagcat taccagaccc 4141 tggtcattca gctccctggc tatccccagc accagacagt gaccacaact gaaatcactc 4201 atcatggaac ctgccaagat gtcaaccata ataaagtaat tgaaaccaac agagaaaatg 4261 acaagcaaga aacatggatg ctgatggagc tgcagaagat tcgcaggcag atagagcact 4321 gcgagggcag gatgactctc aaaaacctcc ctctagcaga ccagggatct tctcaccaca 4381 tcacagtgaa aattaacgag cttaagagtg tgcagaatga ttcacaagca attgctgagg 4441 ttctcaacca gcttaaagat atgcttgcca acttcagagg ttctgaaaag tactgctatt 4501 tacagaatga agtatttgga ctatttcaga aactggaaaa tatcaatggt gttacagatg 4561 gctacttaaa tagcttatgc acagtaaggg cactgctcca ggctattctc caaacagaag 4621 acatgttaaa ggtttatgaa gccaggctca ctgaggagga aactgtctgc ctggacctgg 4681 ataaagtgga agcttaccgc tgtggactga agaaaataaa aaatgacttg aacttgaaga 4741 agtcgttgtt ggccactatg aagacagaac tacagaaagc ccagcagatc cactctcaga 4801 cttcacagca gtatccactt tatgatctgg acttgggcaa gttcggtgaa aaagtcacac 4861 agctgacaga ccgctggcaa aggatagata aacagatcga ctttaggtta tgggacctgg 4921 agaaacaaat caagcaattg aggaattatc gtgataacta tcaggctttc tgcaagtggc 4981 tctatgatgc taaacgccgc caggattcct tagaatccat gaaatttgga gattccaaca 5041 cagtcatgcg gtttttgaat gagcagaaga acttgcacag tgaaatatct ggcaaacgag 5101 acaaatcaga ggaagtacaa aaaattgctg aactttgcgc caattcaatt aaggattatg 5161 agctccagct ggcctcatac acctcaggac tggaaactct gctgaacata cctatcaaga 5221 ggaccatgat tcagtcccct tctggggtga ttctgcaaga ggctgcagat gttcatgctc 5281 ggtacattga actacttaca agatctggag actattacag gttcttaagt gagatgctga 5341 agagtttgga agatctgaag ctgaaaaata ccaagatcga agttttggaa gaggagctca 5401 gactggcccg agatgccaac tcggaaaact gtaataagaa caaattcctg gatcagaacc 5461 tgcagaaata ccaggcagag tgttcccagt tcaaagcgaa gcttgcgagc ctggaggagc 5521 tgaagagaca ggctgagctg gatgggaagt cggctaagca aaatctagac aagtgctacg 5581 gccaaataaa agaactcaat gagaagatca cccgactgac ttatgagatt gaagatgaaa 5641 agagaagaag aaaatctgtg gaagacagat ttgaccaaca gaagaatgac tatgaccaac 5701 tgcagaaagc aaggcaatgt gaaaaggaga accttggttg gcagaaatta gagtctgaga 5761 aagccatcaa ggagaaggag tacgagattg aaaggttgag ggttctactg caggaagaag 5821 gcacccggaa gagagaatat gaaaatgagc tggcaaaggc atctaatagg attcaggaat 5881 caaagaatca gtgtactcag gtggtacagg aaagagagag ccttctggtg aaaatcaaag 5941 tcctggagca agacaaggca aggctgcaga ggctggagga tgagctgaat cgtgcaaaat 6001 caactctaga ggcagaaacc agggtgaaac agcgcctgga gtgtgagaaa cagcaaattc 6061 agaatgacct gaatcagtgg aagactcaat attcccgcaa ggaggaggct attaggaaga 6121 tagaatcgga aagagaaaag agtgagagag agaagaacag tcttaggagt gagatcgaaa 6181 gactccaagc agagatcaag agaattgaag agaggtgcag gcgtaagctg gaggattcta 6241 ccagggagac acagtcacag ttagaaacag aacgctcccg atatcagagg gagattgata 6301 aactcagaca gcgcccatat gggtcccatc gagagaccca gactgagtgt gagtggaccg 6361 ttgacacctc caagctggtg tttgatgggc tgaggaagaa ggtgacagca atgcagctct 6421 atgagtgtca gctgatcgac aaaacaacct tggacaaact attgaagggg aagaagtcag 6481 tggaagaagt tgcttctgaa atccagccat tccttcgggg tgcaggatct atcgctggag 6541 catctgcttc tcctaaggaa aaatactctt tggtagaggc caagagaaag aaattaatca 6601 gcccagaatc cacagtcatg cttctggagg cccaggcagc tacaggtggt ataattgatc 6661 cccatcggaa tgagaagctg actgtcgaca gtgccatagc tcgggacctc attgacttcg 6721 atgaccgtca gcagatatat gcagcagaaa aagctatcac tggttttgat gatccatttt 6781 caggcaagac agtatctgtt tcagaagcca tcaagaaaaa tttgattgat agagaaaccg 6841 gaatgcgcct gctggaagcc cagattgctt cagggggtgt agtagaccct gtgaacagtg 6901 tctttttgcc aaaagatgtc gccttggccc gggggctgat tgatagagat ttgtatcgat 6961 ccctgaatga tccccgagat agtcagaaaa actttgtgga tccagtcacc aaaaagaagg 7021 tcagttacgt gcagctgaag gaacggtgca gaatcgaacc acatactggt ctgctcttgc 7081 tttcagtaca gaagagaagc atgtccttcc aaggaatcag acaacctgtg accgtcactg 7141 agctagtaga ttctggtata ttgagaccgt ccactgtcaa tgaactggaa tctggtcaga 7201 tttcttatga cgaggttggt gagagaatta aggacttcct ccagggttca agctgcatag 7261 caggcatata caatgagacc acaaaacaga agcttggcat ttatgaggcc atgaaaattg 7321 gcttagtccg acctggtact gctctggagt tgctggaagc ccaagcagct actggcttta 7381 tagtggatcc tgttagcaac ttgaggttac cagtggagga agcctacaag agaggtctgg 7441 tgggcattga gttcaaagag aagctcctgt ctgcagaacg agctgtcact gggtataatg 7501 atcctgaaac aggaaacatc atctctttgt tccaagccat gaataaggaa ctcatcgaaa 7561 agggccacgg tattcgctta ttagaagcac agatcgcaac cggggggatc attgacccaa 7621 aggagagcca tcgtttacca gttgacatag catataagag gggctatttc aatgaggaac 7681 tcagtgagat tctctcagat ccaagtgatg ataccaaagg attttttgac cccaacactg 7741 aagaaaatct tacctatctg caactaaaag aaagatgcat taaggatgag gaaacagggc 7801 tctgtcttct gcctctgaaa gaaaagaaga aacaggtgca gacatcacaa aagaataccc 7861 tcaggaagcg tagagtggtc atagttgacc cagaaaccaa taaagaaatg tctgttcagg 7921 aggcctacaa gaagggccta attgattatg aaaccttcaa agaactgtgt gagcaggaat 7981 gtgaatggga agaaataacc atcacgggat cagatggctc caccagggtg gtcctggtag 8041 atagaaagac aggcagtcag tatgatattc aagatgctat tgacaagggc cttgttgaca 8101 ggaagttctt tgatcagtac cgatccggca gcctcagcct cactcaattt gctgacatga 8161 tctccttgaa aaatggtgtc ggcaccagca gcagcatggg cagtggtgtc agcgatgatg 8221 tttttagcag ctcccgacat gaatcagtaa gtaagatttc caccatatcc agcgtcagga 8281 atttaaccat aaggagcagc tctttttcag acaccctgga agaatcgagc cccattgcag 8341 ccatctttga cacagaaaac ctggagaaaa tctccattac agaaggtata gagcggggca 8401 tcgttgacag catcacgggt cagaggcttc tggaggctca ggcctgcaca ggtggcatca 8461 tccacccaac cacgggccag aagctgtcac ttcaggacgc agtctcccag ggtgtgattg 8521 accaagacat ggccaccagg ctgaagcctg ctcagaaagc cttcataggc ttcgagggtg 8581 tgaagggaaa gaagaagatg tcagcagcag aggcagtgaa agaaaaatgg ctcccgtatg 8641 aggctggcca gcgcttcctg gagttccagt acctcacggg aggtcttgtt gacccggaag 8701 tgcatgggag gataagcacc gaagaagcca tccggaaggg gttcatagat ggccgcgccg 8761 cacagaggct gcaagacacc agcagctatg ccaaaatcct gacctgcccc aaaaccaaat 8821 taaaaatatc ctataaggat gccataaatc gctccatggt agaagatatc actgggctgc 8881 gccttctgga agccgcctcc gtgtcgtcca agggcttacc cagcccttac aacatgtctt 8941 cggctccggg gtcccgctcc ggctcccgct cgggatctcg ctccggatct cgctccgggt 9001 cccgcagtgg gtcccggaga ggaagctttg acgccacagg gaattcttcc tactcttatt 9061 cctactcatt tagcagtagt tctattgggc actagGCTCG AATTCACTGG CCGTCGTTTT 9121 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC 9181 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT 9241 GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG 9301 TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG 9361 CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC 9421 ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC 9481 GTCATCACCG AAACGCGCGA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.