pUC57-BMP2K plasmid

pUC57-BMP2K plasmid

PVT106689

pUC57-BMP2K plasmid Information

Plasmid name: pUC57-BMP2K(human)
Alias: NM_001419799.1;BIKE; HRIHFB2017
Gene lenght: 3375
Host: E.coli
Use(s): Gene Template
Fragment type: cDNA
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Non
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13R
Reverse primer:
Copy number: High
Induction:

pUC57-BMP2K plasmid Description

pUC57-BMP2K is a plasmid suitable for gene templates in Escherichia coli.

pUC57-BMP2K plasmid Sequence

1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC 1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 1861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 1921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 1981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 2041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 2101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 2161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 2221 ACCATGATTA CGCCAAGCTT GCATGCAGGC CTCTGCAGTC GACGGGCCCG GGATCCatga 2281 agaagttctc tcggatgccc aagtcggagg gcggcagcgg cggcggagcg gcgggtggcg 2341 gggctggcgg ggccggggcc ggggccggct gcggctccgg cggctcgtcc gtgggggtcc 2401 gggtgttcgc ggtcggccgc caccaggtca ccctggaaga gtcgctggcc gaaggtggat 2461 tctccacagt tttcctcgtg cgtactcacg gtggaatccg atgtgcattg aagcgaatgt 2521 atgtcaataa catgccagac ctcaatgttt gtaaaaggga aattacaatt atgaaagagc 2581 tatctggtca caaaaatatt gtgggctatt tggactgtgc tgttaattca attagtgata 2641 atgtatggga agtccttatc ttaatggaat attgtcgagc tggacaggta gtgaatcaaa 2701 tgaataagaa gctacagacg ggttttacag aaccagaagt gttacagata ttctgtgata 2761 cctgtgaagc tgttgcaagg ttgcatcagt gtaagactcc aataattcac cgggatctga 2821 aggtagaaaa tattttgttg aatgatggtg ggaactatgt actttgtgac tttggcagtg 2881 ccactaataa atttcttaat cctcaaaaag atggagttaa tgtagtagaa gaagaaatta 2941 aaaagtatac aactctgtca tacagagccc ctgaaatgat caacctttat ggagggaaac 3001 ccatcaccac caaggctgat atctgggcac tgggatgtct actctataaa ctttgtttct 3061 tcactcttcc ttttggtgag agtcaggttg ctatctgtga tggcaacttc accatcccag 3121 acaattctcg ttactcccgt aacatacatt gcttaataag gttcatgctt gaaccagatc 3181 cggaacatag acctgatata tttcaagtgt catattttgc atttaaattt gccaaaaagg 3241 attgtccagt ctccaacatc aataattctt ctattccttc agctcttcct gaaccgatga 3301 ctgctagtga agcagctgct aggaaaagcc aaataaaagc cagaataaca gataccattg 3361 gaccaacaga aacctcaatt gcaccaagac aaagaccaaa ggccaactct gctactactg 3421 ccactcccag tgtgctgacc attcaaagtt cagcaacacc tgttaaagtc cttgctcctg 3481 gtgaattcgg taaccataga ccaaaagggg cactaagacc tggaaatggc cctgaaattt 3541 tattgggtca gggacctcct cagcagccgc cacagcagca tagagtactc cagcaactac 3601 agcagggaga ttggagatta cagcaactcc atttacagca tcgtcatcct caccagcagc 3661 agcagcagca gcagcagcaa cagcaacagc agcagcagca acagcaacag cagcagcagc 3721 agcagcagca gcagcaccac caccaccacc accaccacct acttcaagat gcttatatgc 3781 agcagtatca acatgcaaca cagcagcaac agatgcttca acaacaattt ttaatgcatt 3841 cggtatatca accacaacct tctgcatcac agtatcctac aatgatgccg cagtatcagc 3901 aggctttctt tcaacagcag atgctagctc aacatcagcc gtctcaacaa caggcatcac 3961 ctgaatatct tacctcccct caagagttct caccagcctt agtttcctac acttcatcac 4021 ttccagctca ggttggaacc ataatggact cctcctatag tgccaatagg tcagttgctg 4081 ataaagaggc cattgcaaat ttcacaaatc agaagaacat cagcaatcca cctgatatgt 4141 cagggtggaa tccttttgga gaggataatt tctctaagtt aacagaagag gaactattgg 4201 acagagaatt tgaccttcta agatcaagtt ctcctgaaaa gaaagctgaa cattcatcta 4261 taaatcaaga aaatggcact gcaaacccta tcaagaacgg taaaacaagt ccagcatcta 4321 aagatcagcg gactggaaag aaaacctcag tacagggtca agtgcaaaag gggaatgatg 4381 aatctgaaag tgattttgaa tcagatcccc cttctcctaa gagcagtgaa gaggaagagc 4441 aagatgatga agaagttctt cagggggaac aaggagattt taatgatgat gatactgaac 4501 cagaaaatct gggtcatagg cctctcctca tggattctga agatgaggaa gaagaggaga 4561 aacatagctc tgattctgat tatgagcagg ctaaagcaaa gtacagtgac atgagctctg 4621 tctacagaga cagatctggc agtggaccaa cccaagatct taatacaata ctcctcacct 4681 cagcccaatt atcctctgat gttgcagtgg agactcccaa acaggagttt gatgtatttg 4741 gcgctgtccc cttctttgca gtgcgtgctc aacagcccca gcaagaaaag aatgaaaaga 4801 acctccctca acacaggttt cctgctgcag gactggagca ggaggaattt gatgtattca 4861 caaaggcgcc ttttagcaag aaggtgaatg tacaagaatg ccatgcagtg gggcctgagg 4921 cacatactat ccctggttat cccaaaagtg tagatgtatt tggctccact ccatttcagc 4981 ccttcctcac atcaacaagt aaaagtgaaa gcaatgagga cctttttggg cttgtgccct 5041 ttgatgaaat aacggggagc cagcagcaaa aagtcaaaca gcgcagctta cagaaactgt 5101 cctctcgcca aaggcgcaca aagcaggata tgtccaaaag taatgggaag cggcatcatg 5161 gcacgccaac tagcacaaag aagactttga agcctaccta tcgcactcca gagagggctc 5221 gcaggcacaa aaaagtgggc cgccgagact ctcaaagtag caatgaattt ttaaccatct 5281 cagactccaa ggagaacatt agtgttgcac tgactgatgg gaaagatagg gggaatgtct 5341 tacaacctga ggagagcctg ttggacccct tcggtgccaa gcccttccat tctccagacc 5401 tgtcatggca ccctccacat cagggcctga gcgacatccg tgctgatcac aatactgtcc 5461 tgccagggcg gccaagacaa aattcactac atgggtcatt ccatagtgca gatgtattga 5521 aaatggatga ttttggtgcc gtgcccttta cagaacttgt ggtgcaaagc atcactccac 5581 atcagtccca acagtcccaa ccagtcgaat tagacccatt tggtgctgct ccatttcctt 5641 ctaaacagta gGAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC 5701 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA 5761 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCCTG 5821 ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC 5881 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT 5941 GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC 6001 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.