pLV3-CMV-NLS-MCS-mCherry-Puro

pLV3-CMV-NLS-MCS-mCherry-Puro plasmid

PVT106671

pLV3-CMV-NLS-MCS-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-NLS-MCS-mCherry-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NLS-MCS-mCherry-Puro plasmid Description

pLV3-CMV-NLS-MCS-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-NLS-MCS-mCherry-Puro plasmid Sequence

1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 361 gcgctaccgg ACTCAGATCT CGAGCTCAAG CTTCGAATTC TGCAGTCGAC gccaccatgg 421 cgagttcgga ggtttcaatg aaaggtaatc gtggaggaga taacttctcc tcctctggtt 481 ttagtgaccc taaggagact agaaatgtct ccgtcgccgg cgaggggcaa aaaagtaatt 541 ctacccgatc cgctgcaTCT AGAacGGGCC CACCGGTCGC CACCatggtg agcaagggcg 601 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 661 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 721 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 781 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 841 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 901 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca 961 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 1021 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 1081 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 1141 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 1201 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 1261 actccaccgg cggcatggac gagctgtaca agtGAAttct cgacctcgag acaaatggca 1321 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 1381 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 1441 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag 1501 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc 1561 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc 1621 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc 1681 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac 1741 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc 1801 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag 1861 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct 1921 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct 1981 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga 2041 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 2101 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 2161 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 2221 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 2281 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 2341 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 2401 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 2461 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 2521 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 2581 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc 2641 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag 2701 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 2761 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct 2821 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 2881 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 2941 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 3001 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 3061 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 3121 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 3181 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact 3241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 3301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 3361 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg 3421 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 3481 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 3541 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg 3601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 3661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 3721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 3781 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 3841 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 3901 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 3961 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa 4021 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 4081 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 4141 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 4201 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 4261 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 4321 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 4381 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 4441 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 4501 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 4561 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 4621 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 4681 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 4741 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 4801 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 4861 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 4921 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 4981 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 5041 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 5101 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 5161 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 5221 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 5281 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 5341 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 5401 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 5461 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 5521 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 5581 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 5641 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 5701 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 5761 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 5821 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 5881 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 5941 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 6001 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 6061 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 6121 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 6181 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta 6241 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 6301 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 6361 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 6421 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 6481 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 6541 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 6601 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 6661 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 6721 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 6781 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 6841 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 6901 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 6961 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 7021 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 7081 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 7141 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 7201 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 7261 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 7321 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 7381 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 7441 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 7501 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 7561 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 7621 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 7681 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 7741 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 7801 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 7861 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 7921 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 7981 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca 8041 cgagactagc ctcgagcggc cgcccccttc accgagggcc tatttcccat gattccttca 8101 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac 8161 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca 8221 gttttaaaat tatgttttaa aatggactat ca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.