pCMV-3×FLAG-V5-DDX39A-Neo plas

pCMV-3×FLAG-V5-DDX39A-Neo plasmid

PVT106576

pCMV-3×FLAG-V5-DDX39A-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-V5-DDX39A(human)-Neo
Alias: NM_005804.4;BAT1; BAT1L; DDX39; DDXL; URH49
Gene lenght: 1284
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-V5-DDX39A-Neo plasmid Description

pCMV-3×FLAG-V5-DDX39A-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-V5-DDX39A-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt ggtaagccta tccctaaccc tctcctcggt 1021 ctcgattcta caggtaccat ggcagaacag gatgtggaaa acgatctttt ggattacgat 1081 gaagaggaag agccccaggc tcctcaagag agcacaccag ctccccctaa gaaagacatc 1141 aagggatcct acgtttccat ccacagctct ggcttccggg actttctgct gaagccggag 1201 ctcctgcggg ccatcgtgga ctgtggcttt gagcatcctt ctgaggtcca gcatgagtgc 1261 attccccagg ccatcctggg catggacgtc ctgtgccagg ccaagtccgg gatgggcaag 1321 acagcggtct tcgtgctggc caccctacag cagattgagc ctgtcaacgg acaggtgacg 1381 gtcctggtca tgtgccacac gagggagctg gccttccaga tcagcaagga atatgagcgc 1441 ttttccaagt acatgcccag cgtcaaggtg tctgtgttct tcggtggtct ctccatcaag 1501 aaggatgaag aagtgttgaa gaagaactgt ccccatgtcg tggtggggac cccgggccgc 1561 atcctggcgc tcgtgcggaa taggagcttc agcctaaaga atgtgaagca ctttgtgctg 1621 gacgagtgtg acaagatgct ggagcagctg gacatgcggc gggatgtgca ggagatcttc 1681 cgcctgacac cacacgagaa gcagtgcatg atgttcagcg ccaccctgag caaggacatc 1741 cggcctgtgt gcaggaagtt catgcaggat cccatggagg tgtttgtgga cgacgagacc 1801 aagctcacgc tgcacggcct gcagcagtac tacgtcaaac tcaaagacag tgagaagaac 1861 cgcaagctct ttgatctctt ggatgtgctg gagtttaacc aggtgataat cttcgtcaag 1921 tcagtgcagc gctgcatggc cctggcccag ctcctcgtgg agcagaactt cccggccatc 1981 gccatccacc ggggcatggc ccaggaggag cgcctgtcac gctatcagca gttcaaggat 2041 ttccagcggc ggatcctggt ggccaccaat ctgtttggcc gggggatgga catcgagcga 2101 gtcaacatcg tctttaacta cgacatgcct gaggactcgg acacctacct gcaccgggtg 2161 gcccgggcgg gtcgctttgg caccaaaggc ctagccatca cttttgtgtc tgacgagaat 2221 gatgccaaaa tcctcaatga cgtccaggac cggtttgaag ttaatgtggc agaacttcca 2281 gaggaaatcg acatctccac atacatcgag cagagccggt aactcgagtc tagagggccc 2341 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 2401 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 2461 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 2521 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 2581 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 2641 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 2701 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 2761 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 2821 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 2881 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 2941 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 3001 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 3061 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 3121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 3181 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 3241 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 3301 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 3361 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 3421 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg 3481 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 3541 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 3601 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg 3661 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 3721 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 3781 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 3841 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 3901 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 3961 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg 4021 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 4081 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 4141 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 4201 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 4261 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc 4321 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 4381 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 4441 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 4501 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 4561 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 4621 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 4681 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 4741 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 4801 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 4861 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 4921 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 4981 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 5041 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 5101 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 5161 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 5221 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 5281 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 5341 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 5401 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 5461 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 5521 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg 5581 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 5641 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 5701 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 5761 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 5821 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 5881 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 5941 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6001 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6061 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 6121 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 6181 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 6241 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 6301 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 6361 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 6421 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 6481 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 6541 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 6601 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 6661 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 6721 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.