pCMV-HaloTag-EGFP-SQSTM1-Neo p

pCMV-HaloTag-EGFP-SQSTM1-Neo plasmid

PVT106507

pCMV-HaloTag-EGFP-SQSTM1-Neo plasmid Information

Plasmid name: pCMV-HaloTag-EGFP-SQSTM1(human)-Neo
Alias: NM_003900.5;A170; DMRV; EBIAP; FTDALS3; NADGP; OSIL;p60; p62; p62B; PDB3; ZIP3
Gene lenght: 1323
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: HaloTag-F1
Reverse primer:
Copy number: High
Induction:

pCMV-HaloTag-EGFP-SQSTM1-Neo plasmid Description

pCMV-HaloTag-EGFP-SQSTM1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-HaloTag-EGFP-SQSTM1-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaaag 601 ccaccatggc agaaatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg 661 gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc 721 acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc 781 atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt 841 atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag 901 aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca 961 atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc ccgacctggg 1021 acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc 1081 gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc 1141 gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat cctgttgacc 1201 gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg 1261 tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg aagctgctgt 1321 tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc 1381 tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc 1441 cggacctgat cggcagcgag atcgcgcgct ggctgtcgac gctcgagatt tccggcggcg 1501 gtggaggaag cggcggaggt ggatctggta ccgtgagcaa gggcgaggag ctgttcaccg 1561 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 1621 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 1681 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt 1741 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 1801 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 1861 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 1921 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 1981 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 2041 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 2101 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag 2161 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 2221 ctctcggcat ggacgagctg tacaagggcg gtggaggaag cggatccatg gcgtcgctca 2281 ccgtgaaggc ctaccttctg ggcaaggagg acgcggcgcg cgagattcgc cgcttcagct 2341 tctgctgcag ccccgagcct gaggcggaag ccgaggctgc ggcgggtccg ggaccctgcg 2401 agcggctgct gagccgggtg gccgccctgt tccccgcgct gcggcctggc ggcttccagg 2461 cgcactaccg cgatgaggac ggggacttgg ttgccttttc cagtgacgag gaattgacaa 2521 tggccatgtc ctacgtgaag gatgacatct tccgaatcta cattaaagag aaaaaagagt 2581 gccggcggga ccaccgccca ccgtgtgctc aggaggcgcc ccgcaacatg gtgcacccca 2641 atgtgatctg cgatggctgc aatgggcctg tggtaggaac ccgctacaag tgcagcgtct 2701 gcccagacta cgacttgtgt agcgtctgcg agggaaaggg cttgcaccgg gggcacacca 2761 agctcgcatt ccccagcccc ttcgggcacc tgtctgaggg cttctcgcac agccgctggc 2821 tccggaaggt gaaacacgga cacttcgggt ggccaggatg ggaaatgggt ccaccaggaa 2881 actggagccc acgtcctcct cgtgcagggg aggcccgccc tggccccacg gcagaatcag 2941 cttctggtcc atcggaggat ccgagtgtga atttcctgaa gaacgttggg gagagtgtgg 3001 cagctgccct tagccctctg ggcattgaag ttgatatcga tgtggagcac ggagggaaaa 3061 gaagccgcct gacccccgtc tctccagaga gttccagcac agaggagaag agcagctcac 3121 agccaagcag ctgctgctct gaccccagca agccgggtgg gaatgttgag ggcgccacgc 3181 agtctctggc ggagcagatg aggaagatcg ccttggagtc cgaggggcgc cctgaggaac 3241 agatggagtc ggataactgt tcaggaggag atgatgactg gacccatctg tcttcaaaag 3301 aagtggaccc gtctacaggt gaactccagt ccctacagat gccagaatcc gaagggccaa 3361 gctctctgga cccctcccag gagggaccca cagggctgaa ggaagctgcc ttgtacccac 3421 atctcccgcc agaggctgac ccgcggctga ttgagtccct ctcccagatg ctgtccatgg 3481 gcttctctga tgaaggcggc tggctcacca ggctcctgca gaccaagaac tatgacatcg 3541 gagcggctct ggacaccatc cagtattcaa agcatccccc gccgttgtga gcggccgcga 3601 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 3661 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 3721 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 3781 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta 3841 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 3901 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 3961 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 4021 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 4081 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 4141 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 4201 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 4261 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 4321 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 4381 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 4441 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 4501 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 4561 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 4621 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 4681 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 4741 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 4801 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 4861 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 4921 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 4981 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 5041 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 5101 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 5161 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 5221 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 5281 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 5341 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 5401 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 5461 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 5521 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 5581 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 5641 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 5701 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 5761 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 5821 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 5881 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 5941 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 6001 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 6061 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 6121 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 6181 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 6241 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 6301 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 6361 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 6421 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 6481 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 6541 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 6601 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 6661 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 6721 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 6781 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 6841 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 6901 gataaccgta ttaccgccat gcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.