pCMV-Trak1-3×FLAG-Neo plasmid

pCMV-Trak1-3×FLAG-Neo plasmid

PVT106483

pCMV-Trak1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Trak1(mouse)-3×FLAG-Neo
Alias: NM_175114.4;2310001H13Rik; hyrt; mKIAA1042
Gene lenght: 2817
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Trak1-3×FLAG-Neo plasmid Description

pCMV-Trak1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Trak1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatggc attggcaatt cagctcaggc 781 agccctccag ggcccaacct ctgccaggac tcagccacac actggctggg acagactcat 841 gtgacgtgtg caacagcacc aaccttccag aagttgagat cattagcctg ctggaggaac 901 agctgcccca ttacaagcta agagcggaca ccatctatgg ttacgaccac gatgactggc 961 tgcatacgcc cctcatctcc ccagatgcca acatcgacct cacaactgag cagatcgaag 1021 agacgctgaa atacttcctt ctatgtgccg aaagagttgg ccagatgact aagacataca 1081 atgacattga tgctgtcacg aggcttcttg aggagaaaga gcgggatttg gagctggctg 1141 cgaggatcgg tcagtcattg ttgaagaaga acaagaccct aactgagagg aatgaactgc 1201 tggaagagca ggtggagcac atccgggagg aggtgtctca gctccgacat gagctgtcca 1261 tgaaagacga gctgcttcaa ttctacacca gtgccgctga ggagagcgag cctgagtccg 1321 tctgctcaac cccgctgaag aggaacgagt cctcctcctc cgtccagaat tacttccacc 1381 tggactctct tcagaagaag ctcaaggacc tcgaagagga gaacgttgta cttcgatccg 1441 aggcctgtca gctgaagaca gagaccatca cctatgagga gaaggagcag cagctggtga 1501 acgactgcgt gaaggagctg agggatgcca acgtccagat tgcgagcatc tctgaggagc 1561 tggctaagaa gacggaagat gccgcccgcc agcaggagga gatcacccac ctgctgtcgc 1621 aaatcgtgga cttacagaag aaggcaaaat cttgcgcggt ggaaaatgaa gagcttgtcc 1681 agcacctggg cgctgccaag gacgcccagc gtcaactcac agccgagctc cgagagctgg 1741 aggataagta cgcggagtgc atggagatgc ttcatgaggc tcaggaggag ttgaagaacc 1801 tgcggaacaa gacgatgccc acgtcccggc gctaccactc tctgggcctg ttccccatgg 1861 actcgttggc tgcagaaatc gagggaacta tgcgcaagga gctgcagctg gaagagctgg 1921 agtctccaga catcacccac cagaagcgtg tcttcgagac ggtgaggaac gtcaatcaag 1981 tggtcaagca gagatcacta accccttctc ccatgaacat cccgggctcc aaccagtcct 2041 cagccatgaa ctccctcctg tccagctgcg tcagcacccc tcgctccagc ttctacggca 2101 gcgacgtcag caacgtggtc ctcgataaca aaaccaacag catcctcctg gagaccgagg 2161 cagccgacct ggggaacgag gaccacaata agaagccggg cactccaggc acgccaggct 2221 cccatgacct ggagacggca cttaggcggc tatccctacg ccgggagaac tacctgtctg 2281 agcggaggtt cttcgaggag gagcaggaga ggaagctgcg agagctggct gagaaggggg 2341 agctgcacag tggctcgctc acgcccaccg agagcatcat gtccctggga acacactcac 2401 gcttctccga gttcacgggc ttctctggca tgtccttcag cagccgctcc tacctgcccg 2461 agaagctgca gatcgtgaag ccactcgaag gttcagccac gcttcaccac tggcagcaat 2521 tggcccagcc tcatcttggg ggcatcctgg acccccgtcc tggtgtggtc accaagggct 2581 tccggacttt ggatgttgac ctggatgaag tgtactgcct taacgacttt gaggaagatg 2641 acacaggtga ccacatttct ctggcgggcc tagctacctc cacgccaatt cagcacccgg 2701 agacctcagc gcaccaccct gggaagtgca tgtcacagac caactcaact ttcaccttta 2761 ccacttgccg catcctgcac ccttcagacg agctcactcg ggtcacgcca agccttaact 2821 ctgccccagc tccggcttgt agcagtacca gccacttgaa atccacacca gtggccacgc 2881 catgcacccc ccggagactg agcctggctg agtctttcac aaatgtccgt gagtccacga 2941 caaccatgag cacgtccctg gggctggtat ggttgctgaa ggagcgaggc atctcggcgg 3001 ccgtgtacga cccccagagt tgggacagag ccggcagggg ctccctcctc cactcctaca 3061 cccctaggat ggctgtgatc ccgtccaccc caccaaactc ccctatgcag acaccttcgg 3121 cctccccgcc ctccttcgag ttcaagtgca cgagccctcc ctacaacaac ttcctggctt 3181 ccaagccggc cagctctatc ctgagggagg tgagggaaaa gaggcccgtg cggagcagcg 3241 agagccaaac agatgtgtct gtctccaacc tcaaccttgt ggacaaagtc aggaggtttg 3301 gggtggccag agtggtgaac tcaggacgtg cccgtatccc caccttgact gaggaacagg 3361 gacctctcct ctgtggaccc acagggccag cacaggctct tgtccctggg ggcctggtcc 3421 ccgagggcct gcctctgggg tgccccagtg gcatccgacg gaatcgcagc ttccccacta 3481 tggtcgggtc cagcgtgcag atgagagccc ccgtgatcct cacctcgggc atcttgatgg 3541 gtgctaagct ccccaaacag actagcttgc ggGGATCCGA CTACAAAGAC CATGACGGTG 3601 ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGTAGTGA GGGCCCGATA 3661 TCTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA 3721 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC 3781 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA 3841 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC 3901 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC 3961 CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC 4021 TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG 4081 GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC 4141 GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC 4201 GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCCTGG 4261 AAACGGGGGA GGCTAACTGA AACACGGAAG GAGACAATAC CGGAAGGAAC CCGCGCTATG 4321 ACGGCAATAA AAAGACAGAA TAAAACGCAC GGGTGTTGGG TCGTTTGTTC ATAAACGCGG 4381 GGTTCGGTCC CAGGGCTGGC ACTCTGTCGA TACCCCACCG AGACCCCATT GGGGCCAATA 4441 CGCCCGCGTT TCTTCCTTTT CCCCACCCCA CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT 4501 CGCAGCCAAC GTCGGGGCGG CAGGCCCTGC CATAGCAGAT CTGCGCAGCT GGGGCTCTAG 4561 GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG 4621 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC 4681 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGCA TCCCTTTAGG 4741 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC 4801 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT 4861 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC 4921 TTTTGATTTA TAAGGGATTT TGGGGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 4981 ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 5041 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 5101 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 5161 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 5221 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 5281 TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 5341 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 5401 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 5461 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 5521 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 5581 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 5641 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 5701 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 5761 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 5821 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 5881 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 5941 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 6001 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 6061 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 6121 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 6181 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC 6241 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 6301 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 6361 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 6421 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 6481 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC 6541 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 6601 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC 6661 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 6721 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC 6781 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC 6841 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 6901 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 6961 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 7021 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 7081 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 7141 TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 7201 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 7261 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 7321 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 7381 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 7441 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 7501 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 7561 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 7621 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 7681 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 7741 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 7801 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 7861 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 7921 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 7981 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 8041 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 8101 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 8161 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 8221 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 8281 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 8341 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 8401 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 8461 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 8521 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 8581 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 8641 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 8701 TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT CGACTCTCAG TACAATCTGC 8761 TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG 8821 TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG 8881 AATCTGCTTA GG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.