pCMV-3×Myc-TRIM33-Neo plasmid

pCMV-3×Myc-TRIM33-Neo plasmid

PVT106482

pCMV-3×Myc-TRIM33-Neo plasmid Information

Plasmid name: pCMV-3×Myc-TRIM33(human)-Neo
Alias: NM_015906.4;ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA;TIFGAMMA
Gene lenght: 3381
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×Myc-TRIM33-Neo plasmid Description

pCMV-3×Myc-TRIM33-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×Myc-TRIM33-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttAAACTT AAGCCACCAT GGCGGCGGAA CAAAAACTAA TATCAGAGGA AGATTTGGAA 961 CAGAAACTAA TCAGTGAAGA AGATTTAGAG CAAAAGTTAA TTTCCGAGGA GGACTTATCA 1021 GCGGAAAAGC TTatggcgga aaacaaaggc ggcggcgagg ctgagagcgg cggcgggggc 1081 agcggcagcg cgccggtaac tgccggggcc gccgggcccg ccgcgcagga ggcggagccg 1141 cctctcaccg cggtgctggt ggaggaggag gaggaggaag gcggcagggc cggcgctgag 1201 ggcggcgcgg ccgggcccga cgacgggggg gtggccgcgg cctcctcggg ctcggcccag 1261 gctgcttcat ctcctgcggc ctcagtgggc actggagttg ccgggggcgc agtatcgacg 1321 ccggctccag ctccagcctc ggctcccgct ccgggtccct cggcagggcc gcctcctgga 1381 ccgccagcct cgctcctgga cacctgcgcc gtgtgtcagc agagcttgca gagccggcgt 1441 gaggcggagc ccaagctgct gccctgtctt cactccttct gcctgcgctg cctgcccgag 1501 ccggagcgcc agctcagcgt gcccatcccg gggggcagca acggcgacat ccagcaagtt 1561 ggtgtaatac ggtgcccagt atgccgccaa gaatgcagac agatagacct tgtggataat 1621 tattttgtga aagacacatc tgaagctcct agcagttctg atgaaaaatc agaacaggta 1681 tgtactagtt gtgaagacaa tgcaagtgca gttggctttt gtgtagaatg tggagagtgg 1741 ctatgtaaga catgtatcga agcacatcaa agagtaaaat ttactaaaga tcacttgatc 1801 aggaagaaag aagatgtctc agagtctgtt ggagcatctg gtcaacgccc tgttttctgc 1861 cctgtacaca aacaagaaca gttgaaactt ttctgtgaaa catgtgatag attgacatgt 1921 agagactgtc agctattgga acacaaagaa cataggtatc agtttttgga agaagctttt 1981 caaaatcaga agggtgcaat tgagaatcta ctggcgaaac ttcttgagaa gaagaattat 2041 gttcattttg cagctactca ggtgcagaat aggataaaag aagtaaatga gactaacaaa 2101 cgagtagaac aggaaattaa agtggccatt ttcaccctta tcaatgaaat taataagaaa 2161 ggaaaatctc tcttacaaca gctagagaat gttacaaagg aaagacagat gaagttacta 2221 cagcagcaga atgacatcac aggcctttcc cggcaggtga agcatgttat gaacttcaca 2281 aattgggcaa ttgcaagtgg cagcagcaca gcactactat acagcaagcg actgattact 2341 ttccagttgc gtcatatttt gaaagcacgg tgtgatcctg tccctgctgc taatggagca 2401 atacgtttcc attgtgatcc caccttctgg gcaaagaatg tagtcaattt aggtaatcta 2461 gtaatagaga gtaaaccagc tcctggttat actcctaatg ttgtagttgg gcaagttcct 2521 ccagggacaa accacattag taaaacccct ggacagatta acttagcaca gcttcgactc 2581 cagcacatgc aacaacaagt atatgcacag aaacatcagc agttgcaaca gatgaggatg 2641 cagcaaccac cagcacctgt accaactaca acaacaacaa cacaacagca tcctagacaa 2701 gcagcccctc agatgttaca acaacagcct cctcgattga tcagtgtgca aacaatgcaa 2761 agaggcaaca tgaactgtgg agcttttcaa gcccatcaga tgagactggc tcagaatgct 2821 gccagaatac cagggatacc caggcacagc ggccctcaat attccatgat gcagccacac 2881 ctccaaagac aacactcaaa cccagggcat gctggaccct ttcccgtagt atcggtacac 2941 aacaccacaa tcaacccaac gagccctact acagcaacta tggcaaatgc aaaccgaggt 3001 cccaccagcc catctgttac agcaatagag ctaatcccct cagttaccaa tccagaaaac 3061 cttccatcgc tgccagatat tccacccata cagttggaag atgctggctc aagtagttta 3121 gataatctac taagtagata catctcaggc agtcacctac ccccacagcc tacaagcacc 3181 atgaatcctt ctccaggtcc ctctgccctt tctccgggat catcaggttt atccaattct 3241 cacacacctg tgagaccccc aagtacttct agtactggca gtcgaggcag ctgtgggtca 3301 tcaggaagaa ctgctgagaa gacaagtctt agtttcaaat ctgatcaggt gaaggtcaag 3361 caagaacctg ggactgaaga tgaaatatgt agcttttcag gaggtgtaaa acaagaaaaa 3421 acagaggatg gcaggaggag tgcttgcatg ttgagcagtc ctgagagtag cttgacacca 3481 cctctctcaa ccaacctgca tctagaaagt gaattggatg cattggcaag cctggaaaac 3541 catgtgaaaa ttgaacctgc agatatgaat gaaagctgca aacagtcagg gctcagcagc 3601 cttgttaatg gaaagtcccc aattcgaagc ctcatgcaca ggtcggcaag gattggagga 3661 gatggcaaca ataaagatga tgacccaaat gaagactggt gtgctgtctg ccaaaacgga 3721 ggagatctct tgtgctgcga aaaatgtcca aaggtctttc atctaacttg tcatgttcca 3781 acactactta gctttccaag tggggactgg atatgcacat tttgtagaga tattggaaag 3841 ccagaagttg aatatgattg tgataatttg caacatagta agaaggggaa aactgcgcag 3901 gggttaagcc ccgtggacca aaggaaatgt gaacgtcttc tgctttacct ctattgccat 3961 gaattaagta ttgaattcca ggagcctgtt cctgcttcga taccaaacta ctataaaatt 4021 ataaagaaac caatggattt atccaccgtg aaaaagaagc ttcagaaaaa acattcccaa 4081 cactaccaaa tcccggatga ctttgtggcc gatgtccgtt tgatcttcaa gaactgtgaa 4141 aggtttaatg aaatgatgaa agttgttcaa gtttatgcag acacacaaga gattaatttg 4201 aaggctgatt cagaagtagc tcaggcaggg aaagcagttg cattgtactt tgaagataaa 4261 ctcacagaga tctactcaga caggaccttc gcacctttgc cagagtttga gcaggaagag 4321 gatgatggtg aggtaactga ggactctgat gaagacttta tacagccccg cagaaaacgc 4381 ctaaagtcag atgagagacc agtacatata aagtgaattc tgcagatatc cagcacagtg 4441 gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc 4501 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 4561 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 4621 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 4681 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 4741 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 4801 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 4861 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 4921 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 4981 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 5041 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 5101 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 5161 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 5221 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 5281 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 5341 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 5401 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 5461 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 5521 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca 5581 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 5641 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 5701 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 5761 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 5821 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 5881 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 5941 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 6001 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 6061 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 6121 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 6181 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 6241 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 6301 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 6361 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 6421 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 6481 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 6541 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 6601 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 6661 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 6721 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 6781 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 6841 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 6901 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 6961 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 7021 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 7081 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 7141 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 7201 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7261 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 7321 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 7381 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7441 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7501 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7561 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 7621 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt 7681 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 7741 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 7801 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 7861 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 7921 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 7981 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 8041 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 8101 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 8161 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 8221 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 8281 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 8341 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 8401 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 8461 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 8521 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 8581 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 8641 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 8701 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 8761 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 8821 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 8881 cacctgacgt c

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.