pLV3-CMV-NSMAF-3×FLAG-CopGFP-P

pLV3-CMV-NSMAF-3×FLAG-CopGFP-Puro plasmid

PVT106428

pLV3-CMV-NSMAF-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-NSMAF(human)-3×FLAG-CopGFP-Puro
Alias: NM_001413008.1;FAN; GRAMD5
Gene lenght: 786
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NSMAF-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-NSMAF-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-NSMAF-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgt tttctaaaga atcaaaaatg 2341 ctacaaagaa gtatatcatt ttcaaatatg gctttatcgt cttgtttact tttaccagga 2401 gatgccactg tcataacttc ttcatgggat aataatgtct atttttattc catagcattt 2461 ggaagacgcc aggacacgtt aatgggacat gatgatgctg ttagtaagat ctgttggcat 2521 gacaacaggc tatattctgc atcgtgggac tctacagtga aggtgtggtc tggtgttcct 2581 gcagagatgc caggcaccaa aagacaccac tttgacttgc tggccgagct ggaacatgat 2641 gtcagtgtag atacaatcag tttaaatgct gcaagcacac tgttagtttc cggcaccaaa 2701 gaaggcacag tgaatatttg ggacctcaca acggccacct taatgcacca gattccatgc 2761 cattcaggga ttgtatgtga cactgctttt agcccagata gtcgccatgt cctcagcaca 2821 ggaacagatg gctgtcttaa tgtcattgat gtgcagacag gaatgctcat ctcctccatg 2881 acatcagatg agccccagag gtgctttgtc tgggatggaa attccgtttt atctggcagt 2941 cagtctggtg aactgctcgt ttgggacctc cttggagcaa aaatcagtga gagaatacag 3001 ggccacacag gtgctgtgac atgtatatgg atgaatgaac agtgtagcag tatcatcaca 3061 ggaggggaag acagacaaat tatattctgg aaattgcagt atggatccGA CTACAAAGAC 3121 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg 3181 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 3241 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 3301 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 3361 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 3421 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 3481 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 3541 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 3601 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 3661 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 3721 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 3781 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 3841 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 3901 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 3961 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 4021 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 4081 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 4141 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 4201 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 4261 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 4321 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 4381 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 4441 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 4501 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 4561 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 4621 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 4681 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 4741 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 4801 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 4861 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 4921 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 4981 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 5041 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 5101 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 5161 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 5221 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 5281 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 5341 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 5401 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 5461 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 5521 actgacaatt ccgtggtgtt gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt 5581 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 5641 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctTcgcctt 5701 cgccctcaga cgagtcggat ctccctttgg Gccgcctccc cgcctggtac ctttaagacc 5761 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 5821 agggctaatt cactcccaac gaaGataaga tctgcttttt gcttgtactg ggtctctctg 5881 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 5941 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 6001 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 6061 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 6121 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 6181 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 6241 ttatcatgtc tggctctagc taTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg 6301 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 6361 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 6421 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 6481 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 6541 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 6601 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 6661 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 6721 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 6781 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 6841 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 6901 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 6961 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 7021 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 7081 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 7141 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 7201 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7261 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 7321 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 7381 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 7441 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 7501 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 7561 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 7621 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 7681 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 7741 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 7801 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 7861 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 7921 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 7981 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 8041 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 8101 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 8161 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 8221 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8281 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 8341 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 8401 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 8461 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 8521 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 8581 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 8641 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 8701 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 8761 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 8821 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 8881 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 8941 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 9001 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 9061 ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.