pBOBI-CMV-RUNX1-EGFP-3×HA plas

pBOBI-CMV-RUNX1-EGFP-3×HA plasmid

PVT106019

pBOBI-CMV-RUNX1-EGFP-3×HA plasmid Information

Plasmid name: pBOBI-CMV-RUNX1(human)-EGFP-3×HA
Alias: NM_001001890.3;AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2;EVI-1; PEBP2aB; PEBP2alpha
Gene lenght: 1359
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Bleo
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pBOBI-CMV-RUNX1-EGFP-3×HA plasmid Description

pBOBI-CMV-RUNX1-EGFP-3×HA is a plasmid suitable for protein expression in mammalian cells.

pBOBI-CMV-RUNX1-EGFP-3×HA plasmid Sequence

1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg 481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct 841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga 1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg 1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa 2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa 2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg 3121 ttaactctag agccaccatg cgtatccccg tagatgccag cacgagccgc cgcttcacgc 3181 cgccttccac cgcgctgagc ccaggcaaga tgagcgaggc gttgccgctg ggcgccccgg 3241 acgccggcgc tgccctggcc ggcaagctga ggagcggcga ccgcagcatg gtggaggtgc 3301 tggccgacca cccgggcgag ctggtgcgca ccgacagccc caacttcctc tgctccgtgc 3361 tgcctacgca ctggcgctgc aacaagaccc tgcccatcgc tttcaaggtg gtggccctag 3421 gggatgttcc agatggcact ctggtcactg tgatggctgg caatgatgaa aactactcgg 3481 ctgagctgag aaatgctacc gcagccatga agaaccaggt tgcaagattt aatgacctca 3541 ggtttgtcgg tcgaagtgga agagggaaaa gcttcactct gaccatcact gtcttcacaa 3601 acccaccgca agtcgccacc taccacagag ccatcaaaat cacagtggat gggccccgag 3661 aacctcgaag acatcggcag aaactagatg atcagaccaa gcccgggagc ttgtcctttt 3721 ccgagcggct cagtgaactg gagcagctgc ggcgcacagc catgagggtc agcccacacc 3781 acccagcccc cacgcccaac cctcgtgcct ccctgaacca ctccactgcc tttaaccctc 3841 agcctcagag tcagatgcag gatacaaggc agatccaacc atccccaccg tggtcctacg 3901 atcagtccta ccaatacctg ggatccattg cctctccttc tgtgcaccca gcaacgccca 3961 tttcacctgg acgtgccagc ggcatgacaa ccctctctgc agaactttcc agtcgactct 4021 caacggcacc cgacctgaca gcgttcagcg acccgcgcca gttccccgcg ctgccctcca 4081 tctccgaccc ccgcatgcac tatccaggcg ccttcaccta ctccccgacg ccggtcacct 4141 cgggcatcgg catcggcatg tcggccatgg gctcggccac gcgctaccac acctacctgc 4201 cgccgcccta ccccggctcg tcgcaagcgc agggaggccc gttccaagcc agctcgccct 4261 cctaccacct gtactacggc gcctcggccg gctcctacca gttctccatg gtgggcggcg 4321 agcgctcgcc gccgcgcatc ctgccgccct gcaccaacgc ctccaccggc tccgcgctgc 4381 tcaaccccag cctcccgaac cagagcgacg tggtggaggc cgagggcagc cacagcaact 4441 cccccaccaa catggcgccc tccgcgcgcc tggaggaggc cgtgtggagg ccctacggtg 4501 gaggcggatc aggaggcggt ggatctggat ccATGGTGAG CAAGGGCGAG GAGCTGTTCA 4561 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG 4621 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA 4681 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC 4741 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC 4801 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC 4861 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG 4921 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA 4981 ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC 5041 ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG 5101 GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA 5161 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA 5221 TCACTCTCGG CATGGACGAG CTGTACAAGC TCGAGCCATG GAAGcttTAC CCTTATGACG 5281 TACCTGACTA TGCTGGCGCC TACCCTTATG ACGTACCTGA CTATGCTGGA TACCCTTATG 5341 ACGTACCTGA CTATGCTGGA gatatctaac tgactgaacc ggtggtaccg aggaattaat 5401 tcgctgtctg cgagggccag ctgttggggt gagtactccc tctcaaaagc gggcatgact 5461 tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg 5521 gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca 5581 agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact 5641 ttgcctttct ctccacaggt gtccactccc aggtccaact gcaggtcgag catgcatcta 5701 gggcggccaa ttccgccgat cctggaaaaa catggagcaa tcacaagtag caatacagca 5761 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca 5821 gtcacacctc agacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 5881 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 5941 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 6001 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 6061 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 6121 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 6181 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 6241 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 6301 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 6361 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 6421 cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct 6481 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aaagaagaca 6541 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt gacagaacta 6601 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc 6661 agttgagcca gataagatag aagaggccaa taaaggagag aacaccagct tgttacaccc 6721 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag 6781 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gactgtactg ggtctctctg 6841 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 6901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 6961 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggccgcttt 7021 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 7081 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 7141 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 7201 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 7261 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 7321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 7381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 7441 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac 7501 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 7561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 7621 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 7681 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 7741 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca 7801 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 7861 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 7921 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 7981 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 8041 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 8101 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat 8161 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt 8221 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg 8281 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct 8341 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt 8401 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga 8461 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct 8521 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct 8581 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 8641 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 8701 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 8761 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 8821 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 8881 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 8941 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 9001 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 9061 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 9121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 9181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 9241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 9301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 9361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 9421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg 9481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 9541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 9601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 9661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 9721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 9781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 9841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 9901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 9961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 10021 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 10081 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 10141 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 10201 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 10261 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 10321 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 10381 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 10441 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 10501 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 10561 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 10621 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 10681 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 10741 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 10801 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 10861 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 10921 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 10981 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.