pLV3-CMV-HIF1A-3×Myc-EF1a-CopG

pLV3-CMV-HIF1A-3×Myc-EF1a-CopGFP-Puro plasmid

PVT105965

pLV3-CMV-HIF1A-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HIF1A(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_001530.4;bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha;HIF1; HIF1-ALPHA; MOP1; PASD8
Gene lenght: 2478
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HIF1A-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-HIF1A-3×Myc-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-HIF1A-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg agggcgccgg cggcgcgaac gacaagaaaa agataagttc 2341 tgaacgtcga aaagaaaagt ctcgagatgc agccagatct cggcgaagta aagaatctga 2401 agttttttat gagcttgctc atcagttgcc acttccacat aatgtgagtt cgcatcttga 2461 taaggcctct gtgatgaggc ttaccatcag ctatttgcgt gtgaggaaac ttctggatgc 2521 tggtgatttg gatattgaag atgacatgaa agcacagatg aattgctttt atttgaaagc 2581 cttggatggt tttgttatgg ttctcacaga tgatggtgac atgatttaca tttctgataa 2641 tgtgaacaaa tacatgggat taactcagtt tgaactaact ggacacagtg tgtttgattt 2701 tactcatcca tgtgaccatg aggaaatgag agaaatgctt acacacagaa atggccttgt 2761 gaaaaagggt aaagaacaaa acacacagcg aagctttttt ctcagaatga agtgtaccct 2821 aactagccga ggaagaacta tgaacataaa gtctgcaaca tggaaggtat tgcactgcac 2881 aggccacatt cacgtatatg ataccaacag taaccaacct cagtgtgggt ataagaaacc 2941 acctatgacc tgcttggtgc tgatttgtga acccattcct cacccatcaa atattgaaat 3001 tcctttagat agcaagactt tcctcagtcg acacagcctg gatatgaaat tttcttattg 3061 tgatgaaaga attaccgaat tgatgggata tgagccagaa gaacttttag gccgctcaat 3121 ttatgaatat tatcatgctt tggactctga tcatctgacc aaaactcatc atgatatgtt 3181 tactaaagga caagtcacca caggacagta caggatgctt gccaaaagag gtggatatgt 3241 ctgggttgaa actcaagcaa ctgtcatata taacaccaag aattctcaac cacagtgcat 3301 tgtatgtgtg aattacgttg tgagtggtat tattcagcac gacttgattt tctcccttca 3361 acaaacagaa tgtgtcctta aaccggttga atcttcagat atgaaaatga ctcagctatt 3421 caccaaagtt gaatcagaag atacaagtag cctctttgac aaacttaaga aggaacctga 3481 tgctttaact ttgctggccc cagccgctgg agacacaatc atatctttag attttggcag 3541 caacgacaca gaaactgatg accagcaact tgaggaagta ccattatata atgatgtaat 3601 gctcccctca cccaacgaaa aattacagaa tataaatttg gcaatgtctc cattacccac 3661 cgctgaaacg ccaaagccac ttcgaagtag tgctgaccct gcactcaatc aagaagttgc 3721 attaaaatta gaaccaaatc cagagtcact ggaactttct tttaccatgc cccagattca 3781 ggatcagaca cctagtcctt ccgatggaag cactagacaa agttcacctg agcctaatag 3841 tcccagtgaa tattgttttt atgtggatag tgatatggtc aatgaattca agttggaatt 3901 ggtagaaaaa ctttttgctg aagacacaga agcaaagaac ccattttcta ctcaggacac 3961 agatttagac ttggagatgt tagctcccta tatcccaatg gatgatgact tccagttacg 4021 ttccttcgat cagttgtcac cattagaaag cagttccgca agccctgaaa gcgcaagtcc 4081 tcaaagcaca gttacagtat tccagcagac tcaaatacaa gaacctactg ctaatgccac 4141 cactaccact gccaccactg atgaattaaa aacagtgaca aaagaccgta tggaagacat 4201 taaaatattg attgcatctc catctcctac ccacatacat aaagaaacta ctagtgccac 4261 atcatcacca tatagagata ctcaaagtcg gacagcctca ccaaacagag caggaaaagg 4321 agtcatagaa cagacagaaa aatctcatcc aagaagccct aacgtgttat ctgtcgcttt 4381 gagtcaaaga actacagttc ctgaggaaga actaaatcca aagatactag ctttgcagaa 4441 tgctcagaga aagcgaaaaa tggaacatga tggttcactt tttcaagcag taggaattgg 4501 aacattatta cagcagccag acgatcatgc agctactaca tcactttctt ggaaacgtgt 4561 aaaaggatgc aaatctagtg aacagaatgg aatggagcaa aagacaatta ttttaatacc 4621 ctctgattta gcatgtagac tgctggggca atcaatggat gaaagtggat taccacagct 4681 gaccagttat gattgtgaag ttaatgctcc tatacaaggc agcagaaacc tactgcaggg 4741 tgaagaatta ctcagagctt tggatcaagt taacggatcc GAACAAAAAC TAATATCAGA 4801 GGAAGATTTG GAACAGAAAC TAATCAGTGA AGAAGATTTA GAGCAAAAGT TAATTTCCGA 4861 GGAGGACTTA TAgcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga 4921 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc 4981 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 5041 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 5101 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg 5161 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc 5221 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag 5281 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct 5341 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta 5401 cagatccaag ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg 5461 cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga 5521 gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag 5581 caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt 5641 ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa 5701 cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag 5761 cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg 5821 cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga 5881 gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct 5941 gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat 6001 ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct 6061 gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc 6121 cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg 6181 ctccaccgga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa 6241 tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 6301 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 6361 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 6421 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 6481 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 6541 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 6601 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 6661 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 6721 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 6781 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 6841 cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 6901 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 6961 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 7021 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 7081 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 7141 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 7201 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc 7261 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 7321 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 7381 tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct 7441 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 7501 aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg 7561 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 7621 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 7681 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 7741 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 7801 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 7861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 7921 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt 7981 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 8041 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 8101 tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 8161 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 8221 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 8281 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 8341 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 8401 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 8461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 8521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 8581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 8641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 8701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 8761 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 8821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 8881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 8941 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 9001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 9061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 9121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 9181 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 9241 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 9301 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 9361 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 9421 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 9481 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 9541 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 9601 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 9661 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 9721 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 9781 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 9841 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 9901 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 9961 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 10021 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 10081 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 10141 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 10201 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 10261 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 10321 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac 10381 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 10441 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat 10501 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 10561 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg 10621 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 10681 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 10741 gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.