pLV3-U6-COPS8-shRNA1-CopGFP-Pu

pLV3-U6-COPS8-shRNA1-CopGFP-Puro plasmid

PVT105943

pLV3-U6-COPS8-shRNA1-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-COPS8(human)-shRNA1-CopGFP-Puro
Alias: TTAGCCAGACTGACGGATTAT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLV3-U6-COPS8-shRNA1-CopGFP-Puro plasmid Description

pLV3-U6-COPS8-shRNA1-CopGFP-Puro It is a plasmid suitable for interference silencing in mammalian cells and also suitable for lentivirus packaging.

pLV3-U6-COPS8-shRNA1-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acttgaaagc gaaagggaaa ccagagGAGc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtATCG 1801 GTtaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat 1921 tttatcgata ctagtGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC 1981 AAGGCTGTTA GAGAGATAAT TAGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA 2041 AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT 2101 AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2161 ATCTTGTGGA AAGGACTCAT Cgctagcgaa ttcgTTAGCC AGACTGACGG ATTATTTCAA 2221 GAGAATAATC CGTCAGTCTG GCTAATTTTT ggatccgcgg ccgcGaagga tctgcgatcg 2281 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 2341 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 2401 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 2461 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 2521 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 2581 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 2641 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 2701 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 2761 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc 2821 caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg 2881 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg 2941 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct 3001 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa 3061 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga 3121 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg 3181 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat 3241 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg 3301 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag 3361 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt 3421 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca 3481 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc 3541 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct 3601 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg 3661 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 3721 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 3781 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 3841 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 3901 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 3961 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 4021 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 4081 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 4141 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 4201 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg 4261 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 4321 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 4381 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 4441 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 4501 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 4561 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 4621 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 4681 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 4741 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 4801 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg 4861 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 4921 gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 4981 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 5041 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 5101 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 5161 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 5221 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 5281 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 5341 tatcccgccc ctaactccgC CCATCCCGCC CCTAACTCCG cccagttccg cccattctcc 5401 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 5461 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat 5521 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 5581 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 5641 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 5701 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 5761 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 5821 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 5881 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 5941 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 6001 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 6061 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 6121 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 6181 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 6241 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 6301 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 6361 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 6421 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 6481 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 6541 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 6601 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 6661 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 6721 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 6781 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 6841 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 6901 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 6961 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 7021 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 7081 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 7141 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 7201 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 7261 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 7321 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 7381 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 7441 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 7501 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 7561 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 7621 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 7681 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga 7741 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 7801 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 7861 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg 7921 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 7981 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 8041 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 8101 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.