pLV3-CMV-Luc2-EF1a-CopGFP-Hyg

pLV3-CMV-Luc2-EF1a-CopGFP-Hyg plasmid

PVT105897

pLV3-CMV-Luc2-EF1a-CopGFP-Hyg plasmid Information

Plasmid name: pLV3-CMV-Luc2-EF1a-CopGFP-Hyg
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Green,Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Luc2-EF1a-CopGFP-Hyg plasmid Description

pLV3-CMV-Luc2-EF1a-CopGFP-Hyg It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-Luc2-EF1a-CopGFP-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gccaccATGG AAGATGCCAA AAACATTAAG AAGGGCCCAG CGCCATTCTA 2341 CCCACTCGAA GACGGGACCG CCGGCGAGCA GCTGCACAAA GCCATGAAGC GCTACGCCCT 2401 GGTGCCCGGC ACCATCGCCT TTACCGACGC ACATATCGAG GTGGACATTA CCTACGCCGA 2461 GTACTTCGAG ATGAGCGTTC GGCTGGCAGA AGCTATGAAG CGCTATGGGC TGAATACAAA 2521 CCATCGGATC GTGGTGTGCA GCGAGAATAG CTTGCAGTTC TTCATGCCCG TGTTGGGTGC 2581 CCTGTTCATC GGTGTGGCTG TGGCCCCAGC TAACGACATC TACAACGAGC GCGAGCTGCT 2641 GAACAGCATG GGCATCAGCC AGCCCACCGT CGTATTCGTG AGCAAGAAAG GGCTGCAAAA 2701 GATCCTCAAC GTGCAAAAGA AGCTACCGAT CATACAAAAG ATCATCATCA TGGATAGCAA 2761 GACCGACTAC CAGGGCTTCC AAAGCATGTA CACCTTCGTG ACTTCCCATT TGCCACCCGG 2821 CTTCAACGAG TACGACTTCG TGCCCGAGAG CTTCGACCGG GACAAAACCA TCGCCCTGAT 2881 CATGAACAGT AGTGGCAGTA CCGGATTGCC CAAGGGCGTA GCCCTACCGC ACCGCACCGC 2941 TTGTGTCCGA TTCAGTCATG CCCGCGACCC CATCTTCGGC AACCAGATCA TCCCCGACAC 3001 CGCTATCCTC AGCGTGGTGC CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA 3061 CTTGATCTGC GGCTTTCGGG TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG 3121 CAGCTTGCAA GACTATAAGA TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTTCTT 3181 CGCTAAGAGC ACTCTCATCG ACAAGTACGA CCTAAGCAAC TTGCACGAGA TCGCCAGCGG 3241 CGGGGCGCCG CTCAGCAAGG AGGTAGGTGA GGCCGTGGCC AAACGCTTCC ACCTACCAGG 3301 CATCCGCCAG GGCTACGGCC TGACAGAAAC AACCAGCGCC ATTCTGATCA CCCCCGAAGG 3361 GGACGACAAG CCTGGCGCAG TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTGGA 3421 CTTGGACACC GGTAAGACAC TGGGTGTGAA CCAGCGCGGC GAGCTGTGCG TCCGTGGCCC 3481 CATGATCATG AGCGGCTACG TTAACAACCC CGAGGCTACA AACGCTCTCA TCGACAAGGA 3541 CGGCTGGCTG CACAGCGGCG ACATCGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT 3601 GGACCGGCTG AAGAGCCTGA TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA 3661 GAGCATCCTG CTGCAACACC CCAACATCTT CGACGCCGGG GTCGCCGGCC TGCCCGACGA 3721 CGATGCCGGC GAGCTGCCCG CCGCAGTCGT CGTGCTGGAA CACGGTAAAA CCATGACCGA 3781 GAAGGAGATC GTGGACTATG TGGCCAGCCA GGTTACAACC GCCAAGAAGC TGCGCGGTGG 3841 TGTTGTGTTC GTGGACGAGG TGCCTAAAGG ACTGACCGGC AAGTTGGACG CCCGCAAGAT 3901 CCGCGAGATT CTCATTAAGG CCAAGAAGGG CGGCAAGATC GCCGTGTAAg gatccgcggc 3961 cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag 4021 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg 4081 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag 4141 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca 4201 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct 4261 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct 4321 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg 4381 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg 4441 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac 4501 cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc gccatggaga 4561 tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag 4621 agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga 4681 ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct 4741 accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca 4801 cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg 4861 aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg 4921 tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg 4981 gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact 5041 acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc 5101 agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc 5161 tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg 5221 ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg 5281 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaaaa 5341 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct 5401 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag 5461 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg 5521 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat 5581 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc 5641 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg 5701 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc 5761 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc 5821 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc 5881 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca 5941 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg 6001 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg 6061 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc 6121 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt 6181 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga 6241 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag 6301 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagg 6361 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 6421 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 6481 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 6541 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 6601 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 6661 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 6721 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc 6781 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 6841 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 6901 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcggtacc 6961 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 7021 gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg 7081 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 7141 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 7201 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 7261 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 7321 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 7381 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 7441 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc 7501 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 7561 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 7621 gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 7681 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 7741 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 7801 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 7861 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 7921 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 7981 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 8041 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 8101 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 8161 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 8221 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 8281 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 8341 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 8401 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 8461 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 8521 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 8581 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 8641 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 8701 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 8761 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 8821 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 8881 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 8941 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 9001 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 9061 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 9121 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 9181 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 9241 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 9301 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 9361 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 9421 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 9481 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 9541 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 9601 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 9661 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 9721 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 9781 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 9841 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga 9901 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 9961 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca 10021 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 10081 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 10141 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 10201 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 10261 gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.