pLV3-CMV-SUMO2-P2A-SUMO3-3×Myc

pLV3-CMV-SUMO2-P2A-SUMO3-3×Myc-EF1a-CopGFP-Puro plasmid

PVT105872

pLV3-CMV-SUMO2-P2A-SUMO3-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SUMO2(human)-P2A-SUMO3(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_006937.4;HSMT3; Smt3A; SMT3B; SMT3H2; SUMO3；NM_006936.3;SMT3A; Smt3B; SMT3H1; SUMO-3
Gene lenght: 666
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SUMO2-P2A-SUMO3-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-SUMO2-P2A-SUMO3-3×Myc-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-SUMO2-P2A-SUMO3-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg ccgacgaaaa gcccaaggaa ggagtcaaga ctgagaacaa 2341 cgatcatatt aatttgaagg tggcggggca ggatggttct gtggtgcagt ttaagattaa 2401 gaggcataca ccacttagta aactaatgaa agcctattgt gaacgacagg gattgtcaat 2461 gaggcagatc agattccgat ttgacgggca accaatcaat gaaacagaca cacctgcaca 2521 gttggaaatg gaggatgaag atacaattga tgtgttccaa cagcagacgg gaggtgtcta 2581 cGGATCCGGC GCAACAAACT TCTCTCTGCT GAAACAAGCC GGAGATGTCG AAGAGAATCC 2641 TGGACCGatg tccgaggaga agcccaagga gggtgtgaag acagagaatg accacatcaa 2701 cctgaaggtg gccgggcagg acggctccgt ggtgcagttc aagatcaaga ggcacacgcc 2761 gctgagcaag ctgatgaagg cctactgcga gaggcagggc ttgtcaatga ggcagatcag 2821 attcaggttc gacgggcagc caatcaatga aactgacact ccagcacagc tggagatgga 2881 ggacgaggac accatcgacg tgttccagca gcagacggga ggtgtgccgg agagcagcct 2941 ggcagggcac agtttcggat ccGAACAAAA ACTAATATCA GAGGAAGATT TGGAACAGAA 3001 ACTAATCAGT GAAGAAGATT TAGAGCAAAA GTTAATTTCC GAGGAGGACT TATAgcggcc 3061 gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 3121 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 3181 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 3241 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 3301 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 3361 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 3421 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 3481 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 3541 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 3601 ggcgcctacg ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat 3661 cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga 3721 gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac 3781 cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta 3841 ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac 3901 ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga 3961 ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt 4021 gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg 4081 cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta 4141 cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca 4201 gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct 4261 gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc 4321 tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga 4381 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga 4441 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 4501 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 4561 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 4621 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 4681 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 4741 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 4801 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 4861 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 4921 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 4981 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc 5041 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5101 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 5161 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 5221 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 5281 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 5341 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 5401 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 5461 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 5521 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg 5581 ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat 5641 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 5701 gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt 5761 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 5821 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 5881 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 5941 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 6001 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6061 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6121 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc 6181 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 6241 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat 6301 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 6361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 6421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 6481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 6541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 6601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 6661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 6721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 6781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 6841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 6901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 6961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7141 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 7201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 7261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 7381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 7441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 7501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 7561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 7621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 7681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 7741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 7801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 7861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 7921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 7981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 8101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 8161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 8221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 8281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 8341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 8401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 8461 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 8521 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 8581 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 8641 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt 8701 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 8761 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 8821 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 8881 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.