pLV3-CMV-SUMO2-3×Myc-EF1a-CopG

pLV3-CMV-SUMO2-3×Myc-EF1a-CopGFP-Puro plasmid

PVT99432

pLV3-CMV-SUMO2-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SUMO2(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_006937.4;HSMT3; Smt3A; SMT3B; SMT3H2; SUMO3
Gene lenght: 285bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SUMO2-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-SUMO2-3×Myc-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SUMO2-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg ccgacgaaaa gcccaaggaa ggagtcaaga ctgagaacaa 2341 cgatcatatt aatttgaagg tggcggggca ggatggttct gtggtgcagt ttaagattaa 2401 gaggcataca ccacttagta aactaatgaa agcctattgt gaacgacagg gattgtcaat 2461 gaggcagatc agattccgat ttgacgggca accaatcaat gaaacagaca cacctgcaca 2521 gttggaaatg gaggatgaag atacaattga tgtgttccaa cagcagacgg gaggtgtcta 2581 cggatccGAA CAAAAACTAA TATCAGAGGA AGATTTGGAA CAGAAACTAA TCAGTGAAGA 2641 AGATTTAGAG CAAAAGTTAA TTTCCGAGGA GGACTTATAg cggccgcGaa ggatctgcga 2701 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 2761 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag 2821 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 2881 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct 2941 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg 3001 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt 3061 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct 3121 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt 3181 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga 3241 cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac 3301 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca 3361 gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct 3421 gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga 3481 gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta 3541 cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat 3601 cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa 3661 gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt 3721 gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga 3781 cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat 3841 gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta 3901 ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc 3961 tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct 4021 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt 4081 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc 4141 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga 4201 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga 4261 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt 4321 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca 4381 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt 4441 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt 4501 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct 4561 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc 4621 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat 4681 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 4741 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 4801 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 4861 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 4921 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 4981 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 5041 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 5101 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 5161 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg 5221 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 5281 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 5341 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 5401 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 5461 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 5521 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 5581 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 5641 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 5701 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 5761 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 5821 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 5881 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca 5941 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 6001 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 6061 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 6121 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 6181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 6241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 6301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 6361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 6421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 6481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 6541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 6601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 6661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 6721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 6781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 6841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 6901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 6961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 7141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 7201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 7261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 7321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 7381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 7441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 7501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 7561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 7621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 7681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 7741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 7801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 7861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 7921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 7981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 8101 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 8161 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 8221 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 8281 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 8341 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat 8401 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 8461 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 8521 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.