pUASTattB-TNRC6B plasmid

pUASTattB-TNRC6B plasmid

PVT105753

pUASTattB-TNRC6B plasmid Information

Plasmid name: pUASTattB-TNRC6B(human)
Alias: NM_001162501.2;GDSBA
Gene lenght: 5499
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Hsp
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: UAS/Hsp-F1
Reverse primer:
Copy number: High
Induction:

pUASTattB-TNRC6B plasmid Description

pUASTattB-TNRC6B is a plasmid suitable for protein expression in Escherichia coli.

pUASTattB-TNRC6B plasmid Sequence

1 gctagcggat ccaagcttgc atgcctgcag gtcggagtac tgtcctccga gcggagtact 61 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc 121 ctccgagcgg agactctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc 181 gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa 241 caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt 301 aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt 361 attgaataca agaagagaac tctgaataGC CACCATGAGA GAGAAGGAGC AAGAAAGGGA 421 AGAACAGTTA ATGGAAGACA AGAAAAGGAA GAAAGAGGAT AAAAAGAAAA AAGAAGCCAC 481 TCAGAAGGTC ACGGAACAAA AAACCAAAGT GCCCGAAGTG ACGAAACCAA GTTTAAGCCA 541 ACCAACGGCC GCCAGCCCAA TTGGCAGCTC TCCATCGCCA CCAGTCAATG GTGGCAACAA 601 TGCCAAAAGG GTGGCAGTGC CGAACGGACA ACCGCCAAGC GCCGCCCGCT ACATGCCTCG 661 GGAGGTGCCG CCGCGATTCC GTTGCCAGCA GGACCACAAA GTGTTACTAA AACGTGGGCA 721 GCCCCCTCCA CCGTCCTGCA TGCTCCTTGG GGGTGGGGCA GGGCCTCCTC CCTGCACAGC 781 ACCTGGAGCA AACCCAAACA ACGCACAAGT GACAGGAGCG CTGCTGCAGA GTGAGAGTGG 841 GACTGCGCCA GACTCAACCC TTGGAGGTGC TGCTGCTTCA AATTATGCAA ATTCCACTTG 901 GGGCTCGGGA GCCTCCTCCA ACAACGGCAC CTCCCCCAAC CCAATTCACA TCTGGGACAA 961 GGTGATTGTA GACGGGTCTG ACATGGAAGA GTGGCCTTGT ATTGCCAGCA AAGACACTGA 1021 ATCTTCTTCC GAAAACACCA CCGATAACAA CAGTGCCTCG AACCCTGGCT CTGAGAAGAG 1081 CACTCTGCCA GGAAGCACCA CTAGTAACAA AGGAAAAGGG AGCCAGTGCC AGTCTGCAAG 1141 TTCTGGGAAC GAATGTAATC TTGGGGTCTG GAAATCTGAC CCTAAGGCTA AATCTGTTCA 1201 ATCTTCCAAC TCTACTACAG AGAACAACAA TGGACTAGGA AATTGGAGGA ATGTGAGTGG 1261 TCAGGATAGA ATTGGACCTG GCTCTGGCTT CAGCAACTTT AACCCAAATA GCAACCCATC 1321 TGCCTGGCCA GCACTGGTCC AAGAAGGAAC TTCTAGGAAA GGGGCATTGG AAACAGATAA 1381 TAGTAATTCC AGTGCACAGG TTAGCACAGT AGGTCAGACA TCCAGGGAAC AGCAGTCAAA 1441 GATGGAAAAT GCGGGTGTTA ATTTTGTTGT CTCTGGCAGA GAACAGGCTC AAATTCATAA 1501 CACTGATGGA CCAAAAAATG GAAACACTAA CTCCTTGAAC TTAAGTTCAC CAAACCCCAT 1561 GGAGAATAAG GGAATGCCCT TTGGAATGGG CTTGGGGAAC ACCTCCAGGA GCACTGATGC 1621 CCCTTCACAA AGCACTGGAG ATCGAAAGAC TGGGAGTGTT GGATCTTGGG GTGCAGCTAG 1681 GGGGCCTTCT GGAACTGACA CAGTCTCTGG ACAAAGCAAT TCTGGAAACA ATGGGAACAA 1741 TGGAAAAGAG AGAGAGGACT CCTGGAAAGG AGCTTCTGTT CAGAAATCAA CTGGGTCAAA 1801 AAATGACTCT TGGGACAACA ATAACAGGTC TACGGGTGGG TCCTGGAACT TTGGCCCCCA 1861 GGACTCTAAT GACAACAAAT GGGGTGAAGG GAACAAAATG ACATCTGGGG TCTCTCAGGG 1921 AGAATGGAAA CAGCCGACTG GGTCTGATGA GTTGAAAATT GGAGAATGGA GTGGTCCAAA 1981 CCAACCAAAT TCTAGCACTG GAGCATGGGA CAATCAAAAG GGCCACCCCC TCCCTGAAAA 2041 CCAAGGCAAT GCCCAGGCTC CCTGTTGGGG AAGATCTTCC AGCTCCACAG GAAGTGAAGT 2101 TGGAGGTCAA AGCACTGGAA GCAACCACAA AGCAGGAAGT AGTGACAGTC ATAACTCTGG 2161 CCGTCGGTCG TACAGGCCCA CACATCCTGA TTGTCAGGCT GTCTTGCAGA CTCTTTTGAG 2221 CCGAACTGAT TTGGACCCCA GGGTGCTCTC AAACACTGGC TGGGGCCAAA CTCAAATTAA 2281 GCAGGACACA GTGTGGGACA TTGAAGAGGT GCCAAGGCCT GAGGGGAAAT CTGACAAAGG 2341 AACTGAGGGG TGGGAGAGCG CTGCCACACA GACCAAGAAC TCAGGGGGCT GGGGAGATGC 2401 ACCCAGCCAA AGCAATCAAA TGAAGTCTGG ATGGGGGGAG CTCTCAGCCT CTACAGAGTG 2461 GAAAGACCCC AAGAACACAG GAGGCTGGAA TGACTACAAG AACAACAACT CTTCCAACTG 2521 GGGAGGAGGA CGACCTGATG AAAAGACACC TTCCTCTTGG AATGAGAATC CCAGCAAGGA 2581 TCAGGGGTGG GGAGGTGGAC GCCAGCCCAA TCAAGGATGG TCTTCTGGAA AGAATGGTTG 2641 GGGGGAGGAA GTCGATCAGA CAAAAAACAG CAATTGGGAA AGTTCTGCAA GTAAACCTGT 2701 GTCTGGGTGG GGTGAAGGAG GGCAGAATGA AATCGGGACT TGGGGTAATG GTGGCAATGC 2761 AAGCCTAGCT TCAAAAGGTG GGTGGGAGGA TTGCAAAAGA TCCCCAGCAT GGAATGAGAC 2821 GGGCCGACAG CCCAATTCCT GGAATAAACA ACACCAACAG CAGCAGCCCC CACAGCAGCC 2881 GCCGCCACCA CAACCAGAGG CTTCTGGTTC GTGGGGAGGC CCACCCCCAC CACCTCCAGG 2941 CAACGTTCGA CCTTCCAATT CCAGCTGGAG CAGCGGGCCA CAGCCTGCAA CACCTAAGGA 3001 TGAGGAACCC AGTGGTTGGG AAGAGCCATC CCCACAGTCA ATTAGTCGGA AAATGGACAT 3061 TGATGATGGC ACTTCAGCAT GGGGAGACCC TAACAGTTAT AACTACAAGA ATGTGAATCT 3121 GTGGGATAAG AATTCCCAAG GGGGCCCAGC ACCTCGAGAA CCAAACCTGC CCACCCCAAT 3181 GACCAGTAAA TCGGCATCAG TCTGGAGCAA AAGCACACCA CCTGCTCCAG ATAATGGTAC 3241 TTCCGCTTGG GGTGAGCCAA ATGAAAGCAG TCCTGGGTGG GGCGAGATGG ATGATACAGG 3301 AGCATCGACC ACAGGCTGGG GGAACACGCC CGCCAACGCT CCCAATGCCA TGAAGCCTAA 3361 TTCCAAATCT ATGCAAGACG GCTGGGGGGA GAGTGACGGG CCAGTCACAG GAGCTCGCCA 3421 TCCCAGCTGG GAAGAGGAGG AGGATGGAGG AGTCTGGAAC ACCACTGGCT CTCAGGGCAG 3481 TGCTTCCTCC CACAACTCAG CAAGCTGGGG ACAAGGAGGA AAGAAACAAA TGAAGTGCTC 3541 ACTCAAAGGA GGAAACAATG ATTCATGGAT GAATCCTCTT GCCAAACAGT TTTCAAATAT 3601 GGGATTGCTG AGTCAGACTG AAGATAATCC AAGCAGCAAA ATGGATTTGT CTGTAGGAAG 3661 CCTTTCAGAT AAAAAATTTG ATGTGGACAA GCGAGCGATG AATCTCGGGG ATTTTAATGA 3721 TATCATGAGG AAGGATCGAT CTGGGTTCCG TCCACCTAAT TCCAAAGACA TGGGAACCAC 3781 AGATAGTGGG CCTTATTTTG AGAAGCTGAC TTTGCCTTTC TCCAATCAAG ATGGGTGCCT 3841 TGGGGATGAG GCTCCCTGCT CTCCCTTCTC CCCTTCTCCC AGCTACAAGC TGTCTCCCTC 3901 TGGTTCCACA CTACCCAACG TCAGCCTTGG AGCAATCGGC ACAGGGCTCA ACCCCCAAAA 3961 CTTCGCTGCT AGACAAGGCG GTAGTCATGG TTTGTTTGGA AACAGCACAG CACAATCGAG 4021 AGGTCTGCAC ACACCCGTGC AGCCACTAAA TTCTTCTCCC AGTCTCCGGG CGCAAGTGCC 4081 TCCCCAGTTT ATTTCCCCCC AGGTTTCTGC CTCAATGCTC AAGCAGTTTC CCAACAGTGG 4141 CCTGAGTCCA GGTCTTTTCA ATGTGGGGCC CCAGTTATCT CCTCAACAAA TTGCCATGCT 4201 GAGCCAGCTT CCACAAATTC CCCAGTTTCA GTTGGCATGT CAGCTTCTCT TGCAGCAGCA 4261 GCAACAGCAG CAGTTGTTAC AGAACCAGAG AAAGATTTCT CAAGCTGTAC GCCAACAGCA 4321 AGAGCAGCAG CTGGCTCGAA TGGTGAGTGC ACTGCAGCAG CAGCAGCAGC AGCAGCAGAG 4381 GCAGCCAGGC ATGAAGCACT CGCCCTCTCA TCCTGTTGGG CCCAAGCCGC ATCTGGACAA 4441 CATGGTACCC AACGCATTGA ATGTGGGGCT CCCAGACCTT CAAACCAAAG GGCCAATACC 4501 TGGATATGGT TCTGGCTTCA GCTCTGGCGG CATGGACTAT GGCATGGTTG GTGGGAAGGA 4561 GGCTGGAACC GAGTCTCGCT TTAAACAGTG GACCTCCATG ATGGAGGGGC TGCCCTCTGT 4621 AGCCACACAG GAAGCCAATA TGCACAAAAA TGGCGCTATA GTGGCCCCTG GTAAAACCCG 4681 GGGAGGGTCA CCGTACAACC AGTTTGATAT CATCCCTGGT GACACACTGG GTGGCCATAC 4741 GGGTCCTGCT GGTGATAGCT GGTTACCTGC CAAATCTCCA CCAACAAATA AAATCGGAAG 4801 TAAATCCAGC AATGCCAGTT GGCCTCCAGA ATTCCAACCA GGAGTGCCAT GGAAAGGTAT 4861 CCAAAACATT GACCCTGAAT CTGACCCCTA TGTCACCCCA GGAAGTGTGC TGGGGGGTAC 4921 AGCCACATCT CCCATTGTAG ATACTGACCA CCAACTGCTG CGGGATAACA CCACAGGGTC 4981 TAATTCTTCC CTCAACACCT CGCTGCCTTC ACCTGGTGCC TGGCCCTACA GTGCCTCTGA 5041 CAACTCCTTT ACCAACGTTC ATAGCACTTC AGCAAAGTTC CCTGATTACA AATCAACATG 5101 GTCCCCAGAT CCCATAGGAC ACAACCCCAC TCATCTCTCC AACAAGATGT GGAAAAACCA 5161 TATTTCCTCC AGGAACACTA CACCGCTGCC CCGCCCACCT CCTGGTCTGA CCAACCCCAA 5221 ACCATCATCT CCCTGGAGCA GCACAGCACC CCGATCAGTC AGGGGGTGGG GGACACAGGA 5281 CTCACGGCTC GCCTCGGCCT CTACCTGGAG TGATGGTGGC TCAGTTCGTC CTAGTTACTG 5341 GCTGGTTCTT CACAATCTCA CCCCACAGAT TGATGGGTCA ACCTTGAGAA CGATCTGCAT 5401 GCAGCATGGC CCACTGCTGA CATTCCATCT GAATCTAACC CAGGGCACTG CCCTGATCCG 5461 ATACAGCACC AAACAGGAGG CGGCCAAGGC CCAAACTGCA CTGCACATGT GTGTGTTGGG 5521 AAACACTACC ATCCTTGCTG AGTTTGCCAC TGATGATGAA GTCAGCCGCT TTCTGGCACA 5581 AGCTCAGCCC CCTACACCTG CAGCAACCCC AAGTGCGCCA GCTGCGGGGT GGCAGTCGCT 5641 GGAGACCGGC CAGAACCAGT CAGATCCCGT GGGACCTGCT CTGAATCTTT TTGGTGGGTC 5701 CACTGGGCTC GGGCAGTGGA GCAGCAGCGC TGGTGGCAGC AGCGGGGCCG ATCTTGCTGG 5761 CGCTTCATTG TGGGGGCCCC CAAACTATTC TTCTAGCTTA TGGGGAGTCC CAACGGTGGA 5821 AGATCCCCAT AGGATGGGCA GCCCTGCTCC TTTACTACCT GGTGACCTTC TGGGAGGAGG 5881 GTCGGATTCA ATCTAGtcta gaggatcttt gtgaaggaac cttacttctg tggtgtgaca 5941 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt 6001 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga 6061 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa 6121 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa 6181 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt 6241 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa 6301 gctgcactgc tatacaagaa aattatggaa aaatatttga tgtatagtgc cttgactaga 6361 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 6421 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 6481 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 6541 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 6601 ccactagtgt cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg 6661 cccttgggct ccccgggcgc gtactccacc tcacccatct ggtccatcat gatgaacggg 6721 tcgaggtggc ggtagttgat cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg 6781 aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg 6841 gatacgcggg gcagcgtcag cgggttctcg acggtcacgg cgggcatgtc gacactagtt 6901 ctagccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca 6961 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 7021 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 7081 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 7141 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 7201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 7261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 7321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 7801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 8041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 8101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 8161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 8221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 8281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 8341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 8401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 8461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 8521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 8641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 8701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 8761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 8821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 8881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 9001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 9061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt 9121 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 9181 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 9241 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 9301 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 9361 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 9421 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 9481 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 9541 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 9601 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 9661 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 9721 gtgaattgta atacgactca ctatagggcg aattgggtac gtaccgggcc cctagtatgt 9781 atgtaagtta ataaaaccca tttttgcgga aagtagataa aaaaaacatt tttttttttt 9841 actgcactgg atatcattga acttatctga tcagttttaa atttacttcg atccaagggt 9901 atttgatgta ccaggttctt tcgattacct ctcactcaaa atgacattcc actcaaagtc 9961 agcgctgttt gcctccttct ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc 10021 cgctatctct ttcgccaccg tttgtagcgt tacgtagcgt caatgtccgc cttcagttgc 10081 attttgtcag cggtttcgtg acgaagctcc aagcggttta cgccatcaat taaacacaaa 10141 gtgctgtgcc aaaactcctc tcgcttctta tttttgtttg ttttttgagt gattggggtg 10201 gtgattggtt ttgggtgggt aagcagggga aagtgtgaaa aatcccggca atgggccaag 10261 aggatcagga gctattaatt cgcggaggca gcaaacaccc atctgccgag catctgaaca 10321 atgtgagtag tacatgtgca tacatcttaa gttcacttga tctataggaa ctgcgattgc 10381 aacatcaaat tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa ccgcaattgc 10441 acaaacaaat agtgacacga aacagattat tctggtagct gttctcgcta tataagacaa 10501 tttttgagat catatcatga tcaagacatc taaaggcatt cattttcgac tatattcttt 10561 tttacaaaaa atataacaac cagatatttt aagctgatcc tagatgcaca aaaaataaat 10621 aaaagtataa acctacttcg taggatactt cggggtactt tttgttcggg gttagatgag 10681 cataacgctt gtagttgata tttgagatcc cctatcattg cagggtgaca gcggagcggc 10741 ttcgcagagc tgcattaacc agggcttcgg gcaggccaaa aactacggca cgctccggcc 10801 acccagtccg ccggaggact ccggttcagg gagcggccaa ctagccgaga acctcaccta 10861 tgcctggcac aatatggaca tctttggggc ggtcaatcag ccgggctccg gatggcggca 10921 gctggtcaac cggacacgcg gactattctg caacgagcga cacataccgg cgcccaggaa 10981 acatttgctc aagaacggtg agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa 11041 taaagggtcc aattaccaat ttgaaactca gtttgcggcg tggcctatcc gggcgaactt 11101 ttggccgtga tgggcagttc cggtgccgga aagacgaccc tgctgaatgc ccttgccttt 11161 cgatcgccgc agggcatcca agtatcgcca tccgggatgc gactgctcaa tggccaacct 11221 gtggacgcca aggagatgca ggccaggtgc gcctatgtcc agcaggatga cctctttatc 11281 ggctccctaa cggccaggga acacctgatt ttccaggcca tggtgcggat gccacgacat 11341 ctgacctatc ggcagcgagt ggcccgcgtg gatcaggtga tccaggagct ttcgctcagc 11401 aaatgtcagc acacgatcat cggtgtgccc ggcagggtga aaggtctgtc cggcggagaa 11461 aggaagcgtc tggcattcgc ctccgaggca ctaaccgatc cgccgcttct gatctgcgat 11521 gagcccacct ccggactgga ctcatttacc gcccacagcg tcgtccaggt gctgaagaag 11581 ctgtcgcaga agggcaagac cgtcatcctg accattcatc agccgtcttc cgagctgttt 11641 gagctctttg acaagatcct tctgatggcc gagggcaggg tagctttctt gggcactccc 11701 agcgaagccg tcgacttctt ttcctagtga gttcgatgtg tttattaagg gtatctagca 11761 ttacattaca tctcaactcc tatccagcgt gggtgcccag tgtcctacca actacaatcc 11821 ggcggacttt tacgtacagg tgttggccgt tgtgcccgga cgggagatcg agtcccgtga 11881 tcggatcgcc aagatatgcg acaattttgc tattagcaaa gtagcccggg atatggagca 11941 gttgttggcc accaaaaatt tggagaagcc actggagcag ccggagaatg ggtacaccta 12001 caaggccacc tggttcatgc agttccgggc ggtcctgtgg cgatcctggc tgtcggtgct 12061 caaggaacca ctcctcgtaa aagtgcgact tattcagaca acggtgagtg gttccagtgg 12121 aaacaaatga tataacgctt acaattcttg gaaacaaatt cgctagattt tagttagaat 12181 tgcctgattc cacacccttc ttagtttttt tcaatgagat gtatagttta tagttttgca 12241 gaaaataaat aaatttcatt taactcgcga acatgttgaa gatatgaata ttaatgagat 12301 gcgagtaaca ttttaatttg cagatggttg ccatcttgat tggcctcatc tttttgggcc 12361 aacaactcac gcaagtgggc gtgatgaata tcaacggagc catcttcctc ttcctgacca 12421 acatgacctt tcaaaacgtc tttgccacga taaatgtaag tcttgtttag aatacatttg 12481 catattaata atttactaac tttctaatga atcgattcga tttaggtgtt cacctcagag 12541 ctgccagttt ttatgaggga ggcccgaagt cgactttatc gctgtgacac atactttctg 12601 ggcaaaacga ttgccgaatt accgcttttt ctcacagtgc cactggtctt cacggcgatt 12661 gcctatccga tgatcggact gcgggccgga gtgctgcact tcttcaactg cctggcgctg 12721 gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc taatatcctg cgccagctcc 12781 tcgacctcga tggcgctgtc tgtgggtccg ccggttatca taccattcct gctctttggc 12841 ggcttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc gtacctctca 12901 tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt ggagccgggc 12961 gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt catcctggag 13021 acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc cattctcatc 13081 gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg caaggagtag 13141 ccgacatata tccgaaataa ctgcttgttt ttttttttta ccattattac catcgtgttt 13201 actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat 13261 atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt 13321 gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct 13381 acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca 13441 taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct 13501 ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga 13561 ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct caatagaaat tagtaataaa 13621 tatttgtatg tacaatttat ttgctccaat atatttgtat atatttccct cacagctata 13681 tttattctaa tttaatatta tgacttttta aggtaatttt ttgtgacctg ttcggagtga 13741 ttagcgttac aatttgaact gaaagtgaca tccagtgttt gttccttgtg tagatgcatc 13801 tcaaaaaaat ggtgggcata atagtgttgt ttatatatat caaaaataac aactataata 13861 ataagaatac atttaattta gaaaatgctt ggatttcact ggaactaggc tagcataact 13921 tcgtataatg tatgctatac gaagttat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.