pCMV-FZD3-EGFP-6×His-Neo plasm

pCMV-FZD3-EGFP-6×His-Neo plasmid

PVT105746

pCMV-FZD3-EGFP-6×His-Neo plasmid Information

Plasmid name: pCMV-FZD3(human-toxic-opt)-EGFP-6×His-Neo
Alias: NM_017412.4;Fz-3;
Gene lenght: 1998
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-FZD3-EGFP-6×His-Neo plasmid Description

pCMV-FZD3-EGFP-6×His-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-FZD3-EGFP-6×His-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTgccaccA TGGCTATGAC 901 ATGGATAGTA TTTTCACTTT GGCCTCTCAC AGTGTTCATG GGCCACATAG GAGGGCATTC 961 CCTGTTCTCA TGTGAACCCA TCACCCTTCG GATGTGCCAG GACCTGCCGT ATAATACCAC 1021 CTTCATGCCG AACTTGCTCA ACCATTACGA TCAACAGACT GCAGCCCTCG CAATGGAACC 1081 CTTTCACCCG ATGGTCAACT TGGATTGTTC CCGAGACTTT AGGCCCTTTT TGTGCGCTCT 1141 GTACGCTCCG ATTTGCATGG AGTATGGGAG AGTCACTCTT CCTTGTCGCA GACTCTGTCA 1201 GCGCGCCTAC TCAGAATGTA GCAAACTCAT GGAGATGTTC GGGGTGCCCT GGCCAGAGGA 1261 TATGGAGTGC TCTCGCTTTC CAGATTGTGA CGAGCCCTAT CCCAGGCTGG TTGATCTGAA 1321 TCTGGCCGGC GAGCCAACAG AGGGTGCCCC AGTGGCCGTG CAGAGGGATT ACGGATTCTG 1381 GTGCCCAAGG GAGCTTAAGA TCGATCCCGA TCTGGGATAC TCATTTTTGC ATGTTAGGGA 1441 CTGCAGCCCC CCTTGTCCTA ATATGTATTT CAGACGGGAA GAGCTCAGCT TTGCCCGGTA 1501 TTTCATCGGG CTCATTTCCA TCATCTGTCT GTCAGCTACA CTGTTCACGT TTTTGACTTT 1561 CCTGATTGAC GTGACTCGAT TCCGCTATCC CGAAAGGCCA ATCATTTTTT ACGCCGTGTG 1621 TTACATGATG GTCAGTCTGA TCTTTTTTAT CGGCTTTCTG CTCGAGGACA GGGTCGCTTG 1681 CAACGCCAGC ATCCCTGCTC AATACAAAGC ATCCACCGTG ACCCAGGGAA GCCATAATAA 1741 GGCTTGTACC ATGCTCTTTA TGATCCTGTA CTTTTTCACC ATGGCTGGTA GCGTGTGGTG 1801 GGTGATTCTG ACCATCACCT GGTTTCTGGC CGCTGTGCCA AAGTGGGGAT CCGAGGCAAT 1861 TGAGAAGAAA GCACTGCTCT TCCATGCCAG CGCTTGGGGG ATCCCAGGGA CCCTGACGAT 1921 TATCCTCTTG GCAATGAACA AGATCGAGGG TGATAATATT TCTGGAGTGT GTTTTGTGGG 1981 ACTGTACGAC GTCGACGCAC TGCGATATTT TGTGCTGGCT CCTCTGTGCC TGTATGTCGT 2041 GGTGGGGGTA TCCCTGCTTC TCGCTGGCAT TATCTCTCTG AACCGAGTCA GGATCGAAAT 2101 CCCATTGGAA AAAGAGAATC AGGACAAGCT GGTGAAATTT atgatccgga tcggtgtttt 2161 cagcattctt tatctcgtac cactcttggt tgtaattgga tgctactttt atgagcaagc 2221 ttaccggggc atctgggaaa caacgtggat acaagaacgc tgcagagaat atcacattcc 2281 atgtccatat caggttactc aaatgagtcg tccagacttg attctctttc tgatgaaata 2341 cctgatggct ctcatagttg gcattccctc tgtattttgg gttggaagca aaaagacatg 2401 ctttgaatgg gccagttttt ttcatggtcg taggaaaaaa gagatagtga atgagagccg 2461 acaggtactc caggaacctg attttgctca gtctctcctg agggatccaa atactcctat 2521 cataagaaag tcaaggggaa cttccactca aggaacatcc acccatgctt cttcaactca 2581 gctggctatg gtggatgatc aaagaagcaa agcaggaagc atccacagca aagtgagcag 2641 ctaccacggc agcctccaca gatcacgtga tggcaggtac acgccctgca gttacagagg 2701 aatggaggag agactacctc atggcagcat gtcacgacta acagatcact ccaggcatag 2761 tagttctcat cggctcaatg aacagtcacg acatagcagc atcagagatc tcagtaataa 2821 tcccatgact catatcacac atggcaccag catgaatcgg gttattgaag aagatggaac 2881 cagtgctggt ggaggcggat caggaggcgg tGGATCCatg gtgagcaagg gcgaggagct 2941 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 3001 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 3061 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 3121 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 3181 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 3241 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 3301 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 3361 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 3421 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 3481 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 3541 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 3601 cgggatcact ctcggcatgg acgagctgta caagCTCGAG CACCATCACC ATCACCATta 3661 gTCTAGAGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC 3721 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 3781 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT 3841 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC 3901 TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG 3961 GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 4021 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 4081 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 4141 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 4201 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 4261 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 4321 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 4381 AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 4441 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 4501 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 4561 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 4621 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT 4681 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 4741 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 4801 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 4861 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 4921 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 4981 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 5041 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 5101 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 5161 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 5221 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 5281 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 5341 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 5401 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 5461 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 5521 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 5581 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 5641 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 5701 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 5761 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 5821 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 5881 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 5941 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 6001 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 6061 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 6121 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 6181 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 6241 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 6301 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 6361 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 6421 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 6481 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 6541 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 6601 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 6661 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 6721 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 6781 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 6841 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 6901 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 6961 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 7021 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 7081 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 7141 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 7201 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 7261 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 7321 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 7381 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 7441 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 7501 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 7561 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 7621 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 7681 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 7741 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 7801 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 7861 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 7921 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 7981 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 8041 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG 8101 ACGGATCGGG AGATCTCCCG ATCCCCTATG GT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.