pCMV-Atp1a1-3×HA-Neo plasmid

pCMV-Atp1a1-3×HA-Neo plasmid

PVT105710

pCMV-Atp1a1-3×HA-Neo plasmid Information

Plasmid name: pCMV-Atp1a1(mouse)-3×HA-Neo
Alias: NM_144900.2;Atpa-1
Gene lenght: 3069
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Atp1a1-3×HA-Neo plasmid Description

pCMV-Atp1a1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Atp1a1-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC atggggaagg gggttggacg agacaagtat 961 gagcctgccg ctgtatcgga gcatggtgac aaaaagggca aaaaggccaa gaaggaaagg 1021 gatatggatg agctcaagaa ggaagtgtct atggacgacc ataaactcag cctggatgaa 1081 ctccatcgta aatacggaac agatttgagc cgaggattaa cacctgcaag ggccgctgag 1141 atcctggccc gggatggccc caacgccctc acaccccctc ccactactcc cgaatgggtg 1201 aaattctgcc ggcagctctt tggaggcttt tccatgttac tgtggatcgg ggccattctt 1261 tgtttcctgg cttatggcat ccgaagtgct acagaagagg aaccgccaaa cgatgatctg 1321 tacctcgggg tggtgctctc tgctgtagtc atcataactg gctgtttctc ctattatcaa 1381 gaagcaaaaa gctccaagat catggaatcc ttcaagaaca tggtccctca gcaagccctt 1441 gtgattcgaa atggagagaa aatgagcatc aatgcggagg atgtcgtcgt cggggatctg 1501 gtggaggtga aagggggaga ccggatccct gctgatctta gaatcatatc tgcaaacggc 1561 tgcaaggtgg ataactcctc actcactggt gaatcagaac cccagactcg gtccccggat 1621 ttcacaaacg agaacccctt ggagacaagg aacattgcct tcttctcaac caactgtgtg 1681 gaaggaaccg cacgtggcat cgtcgtgtac actggggacc gcaccgtgat gggcaggatt 1741 gccacgcttg cctctgggct agaaggtggc cagaccccca ttgctgaaga gattgagcat 1801 ttcatccacc tcatcaccgg ggtggccgtg ttcctggggg tgtctttctt catcctttct 1861 ctgatccttg agtacacctg gctcgaggct gtcatcttcc tcattggtat catcgtagcc 1921 aacgtgccag aaggtctgct ggccactgtc acggtctgtc tgacactcac tgccaagcgc 1981 atggcgagga agaactgcct ggtgaagaac ttggaagctg tggagacctt ggggtccaca 2041 tctaccatct gctcagacaa gacgggaact ctgactcaga accggatgac agtggcccac 2101 atgtggtttg acaatcagat ccatgaagct gacaccacgg agaatcagag tggtgtctcc 2161 tttgacaaga cgtcagccac ctggttcgct ctgtccagaa ttgctggtct ctgtaacagg 2221 gcagtgtttc aggctaacca agaaaacctg cctattctta agcgggcagt agcgggagat 2281 gcctctgagt cggcgctctt aaagtgcatt gaagtctgct gcggctccgt gatggagatg 2341 agggagaaat acagcaagat agtggagatt cccttcaact ccaccaacaa gtaccagttg 2401 tccattcaca agaacccaaa tgcatctgag cccaaacacc tgctagtgat gaagggcgcc 2461 ccagaaagga tcctggaccg ctgcagctcc atccttctcc acggcaagga gcagccactg 2521 gatgaagagc tgaaggacgc ttttcagaac gcctacctag agctgggggg ccttggagaa 2581 cgtgtgctag gtttctgcca cctcctcctg cctgacgaac agtttcccga aggcttccag 2641 tttgacactg acgatgtcaa tttccccgtg gataacctct gcttcgtggg tcttatctcc 2701 atgattgacc ctccccgagc tgctgtcccc gatgctgtgg gcaaatgccg cagcgctggg 2761 attaaggtca tcatggttac aggagaccat ccaatcacag cgaaagccat tgccaagggg 2821 gtaggcatta tctcagaagg caatgagact gtggaagaca ttgctgcccg cctcaacatt 2881 ccagtgaacc aggtgaaccc cagagatgcc aaggcctgtg tggtccacgg cagtgacctg 2941 aaggacatga cctccgagga gctggatgac atcttgcggt accacacaga gatcgtcttt 3001 gccaggacct ctcctcagca gaagctcatc attgtggagg gctgccagcg gcagggcgcc 3061 atcgtggccg tcacagggga tggtgtcaat gactctccag ctttgaaaaa ggcagatatt 3121 ggggttgcca tggggattgt tggctctgat gtgtccaagc aagctgctga catgatcctt 3181 ctggatgaca actttgcctc cattgtgact ggagtagagg aaggtcgtct gatttttgat 3241 aacttgaaga aatccatcgc ttacacccta accagtaaca ttccggaaat cacccccttc 3301 ttgatattta ttattgcaaa cattccactg cccctgggga ctgtgaccat cctctgcatt 3361 gacttgggca ctgacatggt tcctgccatc tccctggcct acgagcaagc tgagagcgac 3421 atcatgaaga ggcagcccag aaaccccaaa acggacaaac ttgtgaacga gcgtctgatc 3481 agcatggcct atggacagat cggtatgatc caggccctgg gaggcttctt cacttacttt 3541 gtgattctgg ctgagaacgg tttcctgccc tttcacctgt tgggcatccg agagacctgg 3601 gatgaccgct gggtcaacga tgtggaggac agctacgggc agcagtggac ctacgagcag 3661 aggaagatcg tggagttcac ctgccataca gcgttctttg tcagtattgt ggtagtgcag 3721 tgggccgact tggtcatctg caagaccaga aggaattctg tcttccagca gggaatgaag 3781 aacaagatct tgatatttgg cctctttgaa gagacagccc ttgctgcttt cttatcctac 3841 tgccccggga tgggggcagc ccttaggatg tatcccctca aacctacatg gtggttctgt 3901 gccttcccct actcccttct catctttgtg tatgacgagg tgcggaagct cattatcagg 3961 cggcgccctg gcggctgggt ggagaaggaa acctactacC TCGAGTACCC ATACGACGTA 4021 CCTGATTATG CAACTTATCC TTATGATGTA CCTGATTATG CTAGCTATCC TTATGATGTC 4081 CCAGACTACG CAtgaGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT 4141 TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT 4201 CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT 4261 TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC 4321 AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC 4381 TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 4441 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 4501 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 4561 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 4621 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 4681 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 4741 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 4801 ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA 4861 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 4921 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 4981 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA 5041 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 5101 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT 5161 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT 5221 GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG 5281 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG 5341 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG 5401 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC 5461 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG 5521 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA 5581 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC 5641 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG 5701 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG 5761 CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA 5821 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG 5881 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT 5941 GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT 6001 TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA 6061 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 6121 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 6181 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 6241 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 6301 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 6361 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 6421 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 6481 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 6541 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 6601 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 6661 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 6721 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 6781 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 6841 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 6901 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 6961 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 7021 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 7081 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 7141 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 7201 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 7261 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT 7321 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 7381 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 7441 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 7501 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 7561 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 7621 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 7681 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 7741 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 7801 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 7861 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 7921 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 7981 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 8041 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 8101 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 8161 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 8221 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 8281 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 8341 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 8401 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 8461 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 8521 TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.