pCMV-PAPSS1-EYFP-Neo plasmid

pCMV-PAPSS1-EYFP-Neo plasmid

PVT105633

pCMV-PAPSS1-EYFP-Neo plasmid Information

Plasmid name: pCMV-PAPSS1(human)-EYFP-Neo
Alias: NM_005443.5;ATPSK1; PAPSS; SK1;
Gene lenght: 1872
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Yellow
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PAPSS1-EYFP-Neo plasmid Description

pCMV-PAPSS1-EYFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-PAPSS1-EYFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcGCCACC atggagatcc ccgggagcct 661 gtgcaagaaa gtcaaactga gcaataacgc gcagaactgg ggaatgcaga gagcaaccaa 721 tgtcacctac caagcccatc atgtcagcag gaacaagaga ggtcaggtgg tggggaccag 781 aggtggcttt cgtggttgca cagtttggct aacaggcttg tctggagcgg gaaagactac 841 tgtgagcatg gccttggagg agtacctggt ttgtcatggt attccatgct acactctgga 901 tggtgacaat attcgtcaag gtctcaataa aaatcttggc tttagtcctg aagacagaga 961 agagaatgtt cgacgcatcg cagaagttgc taaactgttt gcagatgctg gcttagtgtg 1021 catcacaagt ttcatatcac cttacactca ggatcgcaac aatgcaaggc aaattcatga 1081 aggtgcaagt ttaccgtttt ttgaagtatt tgttgatgct cctctgcatg tttgtgaaca 1141 gagggatgtc aaaggactct acaaaaaagc ccgggcagga gaaattaaag gtttcactgg 1201 gatcgattct gaatatgaaa agccagaggc ccctgagttg gtgctgaaaa cagactcctg 1261 tgatgtaaat gactgtgtcc agcaagttgt ggaacttcta caggaacggg atattgtacc 1321 tgtggatgca tcttatgaag taaaagaact atatgtgcca gaaaataaac ttcatttggc 1381 aaaaacagat gcggaaacat taccagcact gaaaattaat aaagtggata tgcagtgggt 1441 gcaggttttg gcagaaggtt gggcaacccc attgaatggc tttatgagag agagggagta 1501 cttgcagtgc cttcattttg attgtcttct ggatggaggt gtcattaact tgtcagtacc 1561 tatagttctg actgcgactc atgaagataa agagaggctg gacggctgta cagcatttgc 1621 tctgatgtat gagggccgcc gtgtggccat tcttcgcaat ccagagtttt ttgagcacag 1681 gaaagaggag cgctgtgcca gacagtgggg aacgacatgc aagaaccacc cctatattaa 1741 gatggtgatg gaacaaggag attggctgat tggaggagat cttcaagtct tggatcgagt 1801 ttattggaat gatggtcttg atcagtatcg tcttactcct actgagctaa agcagaaatt 1861 taaagatatg aatgctgatg ctgtctttgc atttcaacta cgcaacccag tgcacaatgg 1921 acatgccctg ttaatgcagg atacccataa gcaacttcta gagaggggct accggcgccc 1981 tgtcctcctc ctccaccctc tgggtggctg gacaaaggat gacgatgttc ctttgatgtg 2041 gcgtatgaag cagcatgctg cagtgttgga ggaaggagtt ctgaatcctg agacgacagt 2101 ggtggccatc ttcccatctc ccatgatgta tgctggacca actgaggtcc agtggcattg 2161 cagagcacgg atggttgcag gagccaactt ttacattgtt ggacgagacc ctgctggcat 2221 gcctcatcca gaaacaggga aggatcttta tgagccaagt catggtgcca aagtgctgac 2281 gatggcccct ggtttaatca ctttggaaat agttcccttt cgagttgcag cttacaacaa 2341 gaaaaagaag cgtatggact actatgactc tgaacaccat gaagactttg aatttatttc 2401 aggaacacga atgcgcaaac ttgctcgaga aggccagaaa ccacctgaag gtttcatggc 2461 tcccaaggct tggaccgtgc tgacagaata ctacaaatcc ttggagaaag ctGAATTCgg 2521 tggaggcgga tcaggaggcg gtggatctCC ggatccaccg gtcgccacca tggtgagcaa 2581 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 2641 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 2701 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 2761 cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt 2821 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 2881 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 2941 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 3001 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 3061 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 3121 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta 3181 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 3241 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga 3301 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 3361 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 3421 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 3481 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta 3541 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 3601 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 3661 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 3721 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 3781 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 3841 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 3901 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 3961 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 4021 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 4081 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 4141 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 4201 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 4261 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 4321 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 4381 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 4441 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 4501 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 4561 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 4621 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 4681 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 4741 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 4801 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 4861 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 4921 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 4981 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 5041 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 5101 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 5161 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 5221 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 5281 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 5341 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 5401 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 5461 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 5521 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 5581 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 5641 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 5701 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 5761 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 5821 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 5881 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 5941 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 6001 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 6061 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 6121 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 6181 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 6241 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 6301 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 6361 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 6421 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 6481 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 6541 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 6601 gataaccgta ttaccgccat gcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.