pCMV-NS(Norovirus GII)-Neo pla

pCMV-NS(Norovirus GII)-Neo plasmid

PVT104920

pCMV-NS(Norovirus GII)-Neo plasmid Information

Plasmid name: pCMV-NS(Norovirus GII)-Neo
Alias: NC_039477.1
Gene lenght: 5100
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: NoroVirus GII type
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pCMV-NS(Norovirus GII)-Neo plasmid Description

pCMV-NS(Norovirus GII)-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-NS(Norovirus GII)-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 Cgccaccatg aagatggcgt ctaacgacgc taccgttgcc gttgcttgca acaacaacaa 961 cgacaaggaa aaatcttcag gtgaaggctt attcacaaat atgtcttcca ccttaaagaa 1021 agccctcggg gctaggccca aacagcctgc ctcgagagac gaatcacaaa agcccccaag 1081 accaccaact cccgagttgg tcaagaggat accccctcct ccacctaatg gcgaagaaga 1141 agaagaacca gtcattaggt atgaggttaa gagtgggatc tctggcctgc ccgagctcac 1201 aacagtcccc caaccggacg tggccaacac agcattcagt gttccaccac tgagcttgag 1261 agaaaacagg gaggccaagg aaccgctaac aggggcaata ttagagatgt gggatggaga 1321 gatataccac tatggcctgt acgtggagaa aggcttagtg ttgggtgtgc acaaaccacc 1381 tgcagccata agcatggcaa gagtggagct gacgccgctg tcattgtact ggcgtgtggt 1441 gtacactccc caatacctca tctcccctga aactctcagg aggctcaacg gagaggcgtt 1501 cccttacacc gccttcgaca acaactgcta tgccttttgc tgctgggtgt tagacctcaa 1561 tgactcatgg cttagcagga ggatggtgca aagaacaacg ggcttcttca gaccttacca 1621 agagtggaac agaaaacccc tgcctaccat ggatgactcc aaaattaaga aggtagcaaa 1681 tatattccta tgttcattgt ccacattatt caccagaccc ataaaagacc tcataggaaa 1741 aattaaacca ttaaacatat tgaacatcct ggcaacgtgt gactggacgt ttgccggaat 1801 agtggagtct ctgatattac ttgctgaact cttcggagtt ttctggacac ccccagatgt 1861 gtctgctatg atcgctccct tactcgggga ctacgagttg caagggccag aagacctcgc 1921 cgttgaactc gtacctgtgg taatgggagg gattggtttg gtgttgggat tcaccaaaga 1981 gaaaattggc aaaatgttgt cctcagcagc atcaacactc agggcttgca aagatcttgg 2041 tgcctatggc ttagagatac tcaaattggt catgaagtgg ttcttcccaa agaaagagga 2101 ggccaacgag ctagccatgg tgagggccat agaggatgcc gtactagatc ttgaggcaat 2161 agaaaataac cacatgacaa ccctgttgaa agacaaagac agcttagcaa catacatgaa 2221 aacactggac atggaggagg agaaagccag aagattgtcc acaaaatctg catcccctga 2281 catagttggg acaatcaacg ccctgctggc tcgaatagca gcggccaggt cattagtcca 2341 cagggccaag gaagagctat ctagcaggat aagaccagta gttgttatga tatctggcaa 2401 accaggaata ggcaaaactc atctggccag ggaggtggca agaaaggtgg catccactct 2461 cacaggggat caaagagtcg gactcatacc aagaaacggt gtggaccatt gggatgcata 2521 caaaggtgag agagtcgtgc tgtgggacga ctatggcatg agtaacccca ttcatgatgc 2581 tcttcgcata caagaattgg ctgatacgtg tccccttacc ttaaattgtg acagaattga 2641 aaataaggga aaagtttttg acagtgaagt cataataatt acaacaaacc ttgccaatcc 2701 agccccactt gattatgtca actttgaggc ctgttccagg agaattgatt tcctggtgta 2761 cgctgaggca ccagaagtag aaaaggcaaa acgggacttt cctggtcagc cagatatgtg 2821 gaaggacgcc ttcaagccgg acttttcaca catcaagcta cagcttgcac ctcagggcgg 2881 ctttgacaag aatggcaaca ccccacacgg gaaaggagtg atgaagaccc tcactaccgg 2941 ttctctgatt gcccgtgcat caggcctact gcatgagagg atggatgaat ttgaactcca 3001 aggtcccaca atcaccacct tcaatttcga ccggaacaga atcacagcat tcaggcaatt 3061 ggctgcagaa aacaagtatg gattggtgga taccatgaaa gttggcaatc aactaaaagg 3121 agtgaaaacc atggaagaac tcaaacaagc aattaggaat gtgaccatca agaggtgccg 3181 gatcatctac ggtggctcca cgtatgacct tgaatctgat ggcaagggca aagttttggt 3241 ggaaaaggtc aagaacacct ctgtgcagac caacaacgag ttggccgggg ccctgcacca 3301 tctcaaacac gcccgaatca ggtactatgt caaatgtgtg caagaagcag tctattccat 3361 catacaaatc gccggcgctg cgtttgtcac cacgcgcatt gcacgccgca tgaacataca 3421 agaactctgg tcgaagccac aattagatca aaatgaatca gagactaagg aagaggcccc 3481 caagtcagaa gatgacgagt tcatcatatc ttctaaggac atcaaggagg aaggaaagaa 3541 gggcaaaaac aaaactggcc gtggcaagaa acacactgca ttctccagca agggtttgag 3601 cgatgaggag tatgacgagt acaagaggat aagagaagag agaaatggga agtactctat 3661 agaggagtat cttcaagaca gagacaggta ctatgaggag ctcgccattg ccaaggccac 3721 ggaagaggat ttctgtgaag aggaggagat caaaatccgc cagagaattt tccgtcccac 3781 caggaaacaa agaaaggaag agagggccac attagggcta gtaacaggtt cggaaatcag 3841 aaaaagaaac cctgatgact tcaaacccaa agggaagctg tgggccgatg acaacagaag 3901 tgttgactac aatgagaaac tggactttga ggccccccca agcatatggt ctaggattgt 3961 gagctttggt tctggctggg gcttctgggt atcaccaagc ctgttcataa catcaactca 4021 tgtaatcccc gcaggcataa cagaagcgtt tggagtcccc atcaaacaaa ttcagatcca 4081 caaatcaggt gaattttgcc gattcagatt cccaaaacca attagaccag atgtgacagg 4141 aatgatcttg gaagaaggtg cgcctgaagg caccgtggca actgtgctca tcaaacgccc 4201 caccggagag ctcatgcctc ttgcagccag aatgggaaca cacgcaacca tgaaaattca 4261 aggccgcatg gttggcggac agatgggtat gttgctcact ggatcaaatg ctaaaggaat 4321 ggacttggga acaactcctg gtgactgtgg ctgtccttac atctataaaa ggggcaatga 4381 ctatatagtc attggggtgc acactgcagc agcccgtggt ggaaacaccg tcatttgtgc 4441 cacacaggga agtgagggtg aggcaactct tgagggtgga gatgacaaag gaacatactg 4501 tggggcaccc attctaggcc ctgggggtgc accaaaattg agcaccaaaa ccaaattttg 4561 gaggtcatcg aacacgcccc ttccaccagg gacatatgag cctgcctacc tcggtggccg 4621 tgacccgcgt gttaagggtg ggccctcctt gcagcaggta atgagagacc agttgaagcc 4681 attcactgaa cccaggggca aacctccaag accaagtgta ttggaagcag ccaaacaaac 4741 cattatcaat gtcctcgaac aaaccctgga ccctccacaa aaatggacat acgcacaggc 4801 gtgtgcctca cttgacaaaa ccacctccag cgggcatccc catcacgtcc gaaagaatga 4861 attctggaat ggtgagacct tcactggtaa attggcagac caagcatcaa aagcaaacct 4921 aatgtttgag gaagggaaac acatgacacc agtgtataca gcagcactca aggacgagct 4981 agtcaagact gagaaaatct atggaaagat caagaagaga ctgctctggg gctctgactt 5041 gtccaccatg atccggtgcg ctaggtcatt tggtggactc atggacgaga tgaaggcaca 5101 ctgcatatca ctcccagtac gagttggcat gaatatgaat gaagatggcc caataatatt 5161 tgagaaacat tccagataca aataccacta tgatgcagac tactctcgtt gggattcaac 5221 acaacagagg gcagtgctag cagcagcctt ggaaatcatg gtcagattct ctgcagaacc 5281 acaattggca caaatagtcg ctgaggatct gctggcccct agtgtagtag atgtaggaga 5341 ctttaaaatc acaataaatg aagggctccc atctggtgtg ccatgcactt ctcaatggaa 5401 ctccatcgca cactggctgc taactctctg tgccttgtct gaagtcacca aactgtcccc 5461 tgacattata caagcaaatt ccatgttctc attttacggt gatgacgaga ttgtcagcac 5521 cgacataaaa ttggaccctg aacagttaac cgccaagttg aaggagtacg gcctgaaacc 5581 aacccgccca gacaagaccg agggacccct gatcatcagt gaagatttga acggactcac 5641 tttcctccga aggacggtga ctcgtgaccc agctggctgg tttggaaaac tggaccaaag 5701 ctcgattttg aggcagatgt actggactag aggaccaaat catgaagacc ccaatgagac 5761 aatgataccc cattctcaaa gacccataca gctcatggca ctgcttggtg aagcctctct 5821 tcacggaccc tctttctaca gtaagatcag taaattggtc ataactgaac tcaaagaagg 5881 tgggatggac ttttacgtgc caaggcagga acccatgttc aggtggatga ggttttctga 5941 cttgagcacg tgggagggcg atcgcaatct ggctcccaat ttcgtgaatg aagatggcgt 6001 cgagtgaCTC GAGTCTAGAG GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC 6061 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC 6121 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG 6181 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA 6241 TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG 6301 GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 6361 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT 6421 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT 6481 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG 6541 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 6601 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC 6661 GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA 6721 GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT 6781 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 6841 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 6901 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 6961 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 7021 GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 7081 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 7141 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 7201 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 7261 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 7321 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 7381 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 7441 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 7501 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 7561 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 7621 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 7681 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 7741 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 7801 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 7861 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 7921 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 7981 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 8041 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 8101 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 8161 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 8221 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 8281 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 8341 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 8401 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 8461 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 8521 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 8581 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 8641 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 8701 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 8761 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 8821 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 8881 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 8941 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 9001 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 9061 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 9121 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 9181 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 9241 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 9301 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 9361 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 9421 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 9481 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 9541 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 9601 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 9661 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 9721 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 9781 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 9841 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 9901 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 9961 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 10021 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 10081 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 10141 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 10201 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 10261 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 10321 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 10381 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 10441 CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.