pCMV-T7-Pold1-3×FLAG-Neo plasm

pCMV-T7-Pold1-3×FLAG-Neo plasmid

PVT104753

pCMV-T7-Pold1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-T7-Pold1(mouse)-3×FLAG-Neo
Alias: NM_011131.3;125kDa
Gene lenght: 3315
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-T7-Pold1-3×FLAG-Neo plasmid Description

pCMV-T7-Pold1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-T7-Pold1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgga ttgtaagcgg cgacaaggac 781 caggccctgg ggtgccccca aagcgggctc gagggcacct ctgggatgag gacgagcctt 841 cgccgtcgca gtttgaggcg aacctggcac tgctggagga aatagaggct gagaaccggc 901 tgcaggaggc agaggaggag ctgcagctgc ccccagaggg caccgtgggt gggcagtttt 961 ccactgcaga cattgaccct cggtggcggc ggcccaccct acgtgccctg gaccccagca 1021 cggagcccct catcttccag cagctggaga ttgaccacta tgtgggctca gcaccacccc 1081 tgccagaagg gcccctgcca tcccggaact cagtgcccat actgagggcc tttggggtca 1141 ccgatgaagg cttctccgtc tgctgccaca tacagggctt tgccccctac ttctacaccc 1201 ccgcgcctcc tggttttggg gccgagcacc tgagtgagct gcagcaggag ctgaacgcag 1261 ccatcagccg ggaccagcgc ggtgggaagg agctctcagg gccggcagtg ctggcaatag 1321 agctatgctc ccgtgagagc atgtttgggt accacggtca tggcccttct ccatttctcc 1381 gcatcaccct ggcactaccc cgccttatgg caccagcccg ccgccttctg gaacagggtg 1441 tccgagtgcc aggcctgggc accccgagct tcgcacccta cgaagccaac gtggactttg 1501 agatccggtt catggtggat gctgacattg tgggatgcaa ctggttggag ctgccagctg 1561 gaaagtacgt tcggagggcg gagaagaagg ccaccctgtg tcagctggag gtggacgtgc 1621 tgtggtcaga tgtgatcagt cacccaccgg aggggcagtg gcagcgcatt gcacccctgc 1681 gtgtgcttag cttcgacatc gagtgtgctg gccgaaaagg catcttccct gagcctgagc 1741 gtgaccccgt gatccagatc tgttctctgg ggctgcgctg gggggagccg gagccattct 1801 tgcgtctggc actcacgctg cggccctgtg cccccatcct gggtgccaaa gtgcagagct 1861 atgagcggga agaagacctg ctccaggcct gggccgactt catccttgcc atggaccctg 1921 acgtgatcac cggctacaac attcagaact ttgacctccc atacctcatc tctcgggcac 1981 aggccctaaa ggtggaccgc ttccctttcc tgggccgcgt gactggtctc cgctccaaca 2041 tccgtgactc ctccttccaa tcaaggcagg tcggccggcg ggacagtaag gtgatcagca 2101 tggtgggtcg cgttcagatg gatatgctgc aggtgctgct tcgggaacac aagctccgct 2161 cctacacgct caacgctgtg agtttccact tcctgggcga gcagaaggag gacgttcagc 2221 acagcatcat caccgacctg cagaatggga acgaacagac gcgccgccgc ctggccgtgt 2281 actgcctgaa ggacgccttt ctgccactcc gactactaga gcgccttatg gtgctggtga 2341 ataatgtgga gatggcgcgt gtcacgggtg taccccttgg gtacctgctc acccggggcc 2401 agcaggtcaa ggtcgtgtct cagctgctgc gccaggccat gcgccagggg ctgctgatgc 2461 ctgtggtgaa gaccgagggc agtgaggact acacgggagc cacagtcatt gagcccctca 2521 aagggtacta tgacgtcccc attgccaccc tggacttctc ctccttgtac ccatccatca 2581 tgatggccca taatctgtgc tacaccacgc tgctccgacc tggggctgcc cagaagctgg 2641 gccttaaacc agatgagttc atcaagacac ccactgggga tgagtttgtg aagtcatctg 2701 tacggaaggg cctcctgccc cagatcctgg agaatctgct gagtgcccgc aagagggcca 2761 aggctgagct ggctcaggag acggaccccc tgcggcgaca ggtcttggac ggccggcaac 2821 tggcactaaa agtgagtgcc aactccgtat atggcttcac tggtgcccag gtgggcaagc 2881 tgccatgttt ggagatctcc cagagtgtca ctgggttcgg gcggcagatg attgagaaaa 2941 ccaagcagct tgtggagtcc aagtacaccg tggaaaatgg ctacgatgcc aacgccaagg 3001 tagtctacgg tgacacggac tctgtgatgt gccggtttgg cgtctcctct gtggctgaag 3061 caatgtctct ggggcgggag gctgcaaact gggtatccag tcacttccca tcacccatcc 3121 ggctggagtt cgagaaggtt tacttcccat acctgctcat cagcaagaag cgctatgctg 3181 gcctgctctt ctcctcccgc tctgatgccc atgacaaaat ggactgcaag ggcctggagg 3241 ctgtgcgcag ggacaactgt cccctggtgg ccaacctcgt tacatcctct ctgcgccgga 3301 tcctcgtgga ccgggaccct gatggggcag tagcccatgc caaggacgtc atctcggacc 3361 tgctgtgcaa ccgcatagac atctcccagc tggtcatcac caaagagttg acccgcgcag 3421 cagcagacta tgctggcaag caggctcacg tggagctggc tgagaggatg aggaagcgcg 3481 accccggcag tgcgcccagc ctgggtgacc gagtccccta tgtgatcatt ggtgctgcta 3541 agggtgtggc cgcctacatg aagtcggagg accccctgtt tgtgctggag cacagcctgc 3601 ccatcgacac tcagtactac ctggagcagc agctggccaa gccgctcttg cgcatctttg 3661 agcccatcct gggtgagggc cgtgcagagt ctgtgctgct gcgcggtgac cacacacgat 3721 gcaagactgt gctcaccagc aaggtgggcg gcctcttggc cttcaccaag cgccgcaact 3781 gttgcattgg ctgccgctcc gtaatcgacc atcaaggagc cgtgtgtaag ttctgtcagc 3841 cacgggagtc ggagctctat cagaaggagg tgtcacacct gaatgccttg gaagaacggt 3901 tctctcgcct ctggacacag tgtcaacgct gccagggcag cttgcatgag gacgtcatct 3961 gtaccagccg tgactgtccc atcttctaca tgcgcaagaa ggtgcgcaag gacctggaag 4021 accaggaacg gctgctgcag cgctttggac cgcccggccc tgaggcctgg GGATCCGACT 4081 ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA 4141 AGTAGTGAGG GCCCGATATC TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT 4201 GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC 4261 TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG 4321 GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC 4381 TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC 4441 CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC 4501 CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA 4561 GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC 4621 CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC 4681 GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG 4741 GGCCGCCTCC CCGCCTGGAA ACGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG 4801 GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG GTGTTGGGTC 4861 GTTTGTTCAT AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG 4921 ACCCCATTGG GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC 4981 GGGTGAAGGC CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCAGATCT 5041 GCGCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC 5101 GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC 5161 TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA 5221 TCGGGGCATC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT 5281 TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT 5341 GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA 5401 CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG GGGATTTCGG CCTATTGGTT 5461 AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG 5521 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 5581 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 5641 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 5701 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 5761 GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 5821 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 5881 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 5941 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 6001 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 6061 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 6121 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 6181 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 6241 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 6301 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 6361 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 6421 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 6481 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 6541 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 6601 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 6661 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 6721 CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC 6781 CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA 6841 GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA 6901 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA 6961 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC 7021 CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 7081 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 7141 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 7201 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 7261 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 7321 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 7381 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 7441 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 7501 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 7561 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 7621 TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 7681 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 7741 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 7801 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 7861 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA 7921 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 7981 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 8041 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 8101 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 8161 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 8221 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 8281 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 8341 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 8401 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 8461 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 8521 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 8581 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 8641 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 8701 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 8761 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 8821 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 8881 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 8941 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 9001 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 9061 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 9121 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 9181 TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGTCG 9241 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATCTGCT CCCTGCTTGT 9301 GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC AAAATTTAAG CTACAACAAG GCAAGGCTTG 9361 ACCGACAATT GCATGAAGAA TCTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.